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Method for improving content of ganoderma triterpenes in ganoderma liquid deep fermentation mycelium

A liquid submerged fermentation, Ganoderma lucidum triterpenoid technology, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve problems such as hindering the application of Ganoderma lucidum products, and achieve the effects of shortening the fermentation period, promoting fermentation, and increasing yield

Inactive Publication Date: 2014-09-03
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the yield of Ganoderma lucidum and its key medicinal components has become a bottleneck hindering the application of Ganoderma lucidum products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Take the slant mycelium Hunong Ganoderma lucidum No. 1 strain activated in the slant medium (potato dextrose agar (produced by U.S. BD company) powder 39g dissolved in 1L deionized water and sterilized), and pick soybeans The mycelium pieces of grain size were inserted into 100mL seed medium (soybean cake powder 20g / L, anhydrous glucose 25g / L, magnesium sulfate heptahydrate 1.5g / L, potassium dihydrogen phosphate 3.0g / L, natural pH), inoculated Then carry out fermentation and cultivation at 26°C and 150r / min for 14 days;

[0021] (2) The cultivated seed culture solution is inserted into the fermentation medium according to the inoculum size of 10% (bean cake powder 30g / L, soluble starch 20g / L, magnesium sulfate heptahydrate 2.25g / L, potassium dihydrogen phosphate 1.5g / L, natural pH), cultivated for 10 days at 26°C and 150r / min after inoculation to obtain Ganoderma lucidum triterpenes.

[0022] In the fermentation process described in step (2), the method of adding ...

Embodiment 2

[0025] (1) Take the slant mycelium Hunong Ganoderma lucidum No. 1 strain activated in the slant medium (potato dextrose agar (produced by U.S. BD company) powder 39g dissolved in 1L deionized water and sterilized), and pick soybeans The mycelium pieces of grain size were inserted into 100mL seed medium (soybean cake powder 20g / L, anhydrous glucose 25g / L, magnesium sulfate heptahydrate 1.5g / L, potassium dihydrogen phosphate 3.0g / L, natural pH), inoculated Then carry out fermentation and cultivation at 26°C and 150r / min for 14 days;

[0026] (2) The cultivated seed culture solution is inserted into the fermentation medium according to the inoculum size of 10% (bean cake powder 30g / L, soluble starch 20g / L, magnesium sulfate heptahydrate 2.25g / L, potassium dihydrogen phosphate 1.5g / L, natural pH), cultivated for 10 days at 26°C and 150r / min after inoculation to obtain Ganoderma lucidum triterpenes.

[0027] In the fermentation process described in step (2), the method of adding ...

Embodiment 3

[0030] (1) Take the slant mycelium Hunong Ganoderma lucidum No. 1 strain activated in the slant medium (potato dextrose agar (produced by U.S. BD company) powder 39g dissolved in 1L deionized water and sterilized), and pick soybeans The mycelium pieces of grain size were inserted into 100mL seed medium (soybean cake powder 20g / L, anhydrous glucose 25g / L, magnesium sulfate heptahydrate 1.5g / L, potassium dihydrogen phosphate 3.0g / L, natural pH), inoculated Then carry out fermentation and cultivation at 26°C and 150r / min for 14 days;

[0031] (2) The cultivated seed culture solution is inserted into the fermentation medium according to the inoculum size of 10% (bean cake powder 30g / L, soluble starch 20g / L, magnesium sulfate heptahydrate 2.25g / L, potassium dihydrogen phosphate 1.5g / L, natural pH), cultivated for 10 days at 26°C and 150r / min after inoculation to obtain Ganoderma lucidum triterpenes.

[0032] In the fermentation process described in step (2), the method of adding ...

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Abstract

The invention relates to a method for improving content of ganoderma triterpenes in ganoderma liquid deep fermentation mycelium, and the method is as follows: 1, taking a slant mycelium ganoderma strain, picking a sclertium for inoculating into a seed culture medium, after the inoculation, culturing for 7-14 days to obtain a seed culture liquid; 2, inoculating the cultured seed culture liquid into a fermentation medium according to the inoculum concentration of 5%-15%, after the inoculation, culturing for 3 to 10 days, adding 1vol%-5vol% of oleic acid in the fermentation culturing process to finally obtain ganoderma mycelium for further extract of the ganoderma triterpenes. The method has the advantages of being simple in process and effective, has application value in the industrial production, can successfully achieve significant improvement of yield of an expression product, shortens the fermentation cycle, improves the production efficiency, and has certain guidance and inspiration significance to optimize the fermentation process of other fungal mycelium.

Description

technical field [0001] The invention belongs to the field of ganoderma lucidum triterpene fermentation, in particular to a method for increasing the content of ganoderma lucidum triterpenoids in ganoderma lucidum liquid submerged fermentation mycelia. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is a fungus belonging to Basidiomycetes, Polyporaceae, and Ganoderma genus. It is the most famous higher medicinal fungus in my country. The key medicinal components of Ganoderma lucidum are polysaccharides and triterpenes of Ganoderma lucidum. Among them, Ganoderma lucidum triterpenes have anti-inflammatory, analgesic, sedative, detoxifying, liver-protecting, anti-HIV, anti-tumor, liver-protecting effects, inhibit histamine release, angiotensin-converting enzyme and other functions, and are an important material basis for Ganoderma lucidum to exert its medicinal effects. [0003] At present, the yield of Ganoderma lucidum and its key medicinal components has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12N1/14C12R1/645
Inventor 冯杰张劲松杨焱贾薇冯娜刘方
Owner SHANGHAI ACAD OF AGRI SCI
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