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Bacillus subtilis cultured by biochar and preparation method and application thereof

A technology of Bacillus subtilis and biochar, which is applied in the field of Bacillus subtilis and its preparation, can solve problems such as carbon instability, and achieve the effect of increasing the density of bacteria and increasing the concentration of dissolved oxygen

Inactive Publication Date: 2017-02-08
HEHAI TECHNOLOGY ENGINEERING GROUP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is a large amount of carbon naturally present in the land, but this carbon is unstable and will be released by the climate

Method used

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  • Bacillus subtilis cultured by biochar and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Such as figure 1 Shown, a kind of preparation method utilizing the Bacillus subtilis cultivated by biochar specifically comprises the steps:

[0036] (1) Test tube expansion culture: Inoculate a ring of Bacillus sublitis ACCC19742 slant strain into the Bacillus subtilis test tube slant medium, cultivate at 25°C for 10 days, and obtain the test tube slant strain. The test tube slant is 4 It is preserved in ℃ for subsequent use; the test tube slant bacterial classification is the general nutrient agar (2) medium (Edited by Chen Tianshou. The manufacture and application of microbial culture medium, China Agricultural Press, p182 in 1995);

[0037] (2) Liquid shake flask culture: inoculate the test tube slant bacterial strain that the one-ring step (1) makes is inoculated in the 250mL Erlenmeyer flask that 20mL containing the liquid shake flask culture medium that biochar is housed, and the Erlenmeyer flask is rotated at 10 revolutions / min, under the condition of temperat...

Embodiment 2

[0046] Such as figure 1 Shown, a kind of preparation method utilizing the Bacillus subtilis cultivated by biochar specifically comprises the steps:

[0047] (1) Test tube expansion culture: inoculate a ring of endogenous Bacillus subtilis (Bacillus subtilis ACCC19743) slant strains into the Bacillus subtilis test tube slant medium, and cultivate at 40°C for 2 days to obtain test tube slant strains. The test tube slant is 4 Preserve for subsequent use at ℃; the test tube slant bacterial classification is the general Bacillus subtilis culture medium (edited by Chen Tianshou. The manufacture and application of microbial culture medium, China Agricultural Press, p182 in 1995);

[0048] (2) Liquid shake flask culture: Inoculate the test tube slant bacterial strain that one ring step (1) makes is inoculated in the 250mL Erlenmeyer flask that 150mL containing the liquid shake flask culture medium that biochar is housed, and the Erlenmeyer flask rotates at 160 rpm / min, at a temperat...

Embodiment 3

[0057] Such as figure 1 Shown, a kind of preparation method utilizing the Bacillus subtilis cultivated by biochar specifically comprises the steps:

[0058] (1) Test tube expansion culture: Inoculate a ring of Bacillus subtilis (CICC 20034) slant strains into the Bacillus subtilis test tube slant medium, cultivate at 32°C for 6 days to obtain test tube slant strains, and the test tube slant is 4°C Preserve for subsequent use; Described test tube slant strain is the general Bacillus subtilis culture medium that adopts (Chen Tianshou editor-in-chief. The manufacture and application of microbial culture medium, China Agricultural Press, 1995 p182);

[0059] (2) Liquid shake flask culture: Inoculate the test tube slant bacterial strain that step (1) makes inoculate in the 250mL Erlenmeyer flask that 80mL liquid shake flask culture medium that contains biochar is housed, and the Erlenmeyer flask rotates at 85 rpm / min, under the condition of temperature 32°C, cultured on a shaker ...

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Abstract

The invention discloses bacillus subtilis cultured by biochar and a preparation method and an application thereof. The preparation method takes biochar as an adsorption carrier, such as wood chips, animal bones, plant rhizomes and other biochar, takes any kind of bacillus subtilis strains as a starting strain, and successively comprises the following steps: (1) test tube expanding culture; (2) liquid shake flask culture; (3) seed tank expanding culture; (4) fermentation culture; and (5) drying. The bacterial density of the bacillus subtilis prepared by the method can reach 5*10<13>-1*10<15> cfu / mL. The biological adsorption and the liquid fermentation are in organic combination, the bacterial density of the liquid fermentation is improved, no any additional equipment is added, continuous culture and semi continuous culture are not used, and the high-density culture of the bacillus subtilis is achieved; and the cultured bacillus subtilis can be used as a live bacterial agent of a biofertilizer or used as a live bacterial agent of the biofertilizer with other strains.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a bacillus subtilis cultivated by using biochar, a preparation method and application thereof. Background technique [0002] The so-called biochar is made of organic waste, such as animal manure, animal bones, plant roots, wood chips and wheat straw, which are decomposed by high temperature through thermochemical effects, and become carbon-like spherical substances that can be used as fertilizers. Among them, the charcoal formed by plants for the purpose of fixing carbon is called "biological carbon". There is a large amount of carbon naturally present in the land, but this carbon is unstable and will release carbon dioxide due to the impact of climate. Biochar, on the other hand, can fix carbon for hundreds of years. [0003] People living in the Brazilian Amazon have long used a special kind of fertilizer. This fertilizer is locally sourced and has a strong ability to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/125
CPCC12N1/20C05F11/08
Inventor 俞建国
Owner HEHAI TECHNOLOGY ENGINEERING GROUP CO LTD
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