Preparation method of composite bacterial agent of ammonia oxidation bacteria

A technology of ammonia oxidizing bacteria and compound bacterial agent, applied in the field of environmental biology, can solve the problems of large investment and high production cost of bacterial agent, and achieve the effect of improving ammonia nitrogen removal efficiency

Inactive Publication Date: 2013-04-17
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional microbial preparation method needs to specially prepare a suitable medium, use special microbial fermentation equipment to cultivate a large number of required microbial cells, and then add appropriate auxiliary agents to the culture; the production cost of the bacterial agent is relatively high. Large investment for large-scale sewage treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: The preparation of the ammonia oxidizing bacteria composite bacterial agent that is used for short-range nitrification process

[0015] (1) Adjust the short-range nitrification bioreactor for ammonia nitrogen wastewater treatment to achieve high-efficiency ammonia nitrogen removal process conditions and maintain stable operation.

[0016] (2) Without reducing the treatment efficiency of the bioreactor, 3% of the activated sludge returned to the sludge sedimentation tank is drawn out of the system to obtain a composite microbial culture rich in ammonia-oxidizing bacteria.

[0017] (3) With biochar passed through a 30-mesh sieve as a carrier, the composite microbial culture of ammonia oxidizing bacteria and biochar are mixed evenly in a weight ratio of 1:3, and ventilated and dried at room temperature until the water content is below 20% to obtain ammonia oxidation. Bacteria complex agent, packaged and stored in a cool and dry place.

[0018] (4) When start...

Embodiment 2

[0019] Embodiment 2: the preparation of the ammonia oxidizing bacteria composite bacteria agent that is used for A / O process

[0020] (1) Adjust the process conditions of ammonia nitrogen wastewater treatment A / O bioreactor to achieve efficient ammonia nitrogen removal and maintain stable operation.

[0021] (2) Without reducing the treatment efficiency of the bioreactor, 5% of the activated sludge returned to the sludge settling tank is drawn out of the system to obtain a composite microbial culture rich in ammonia-oxidizing bacteria.

[0022] (3) With biochar passed through a 30-mesh sieve as a carrier, the composite microbial culture of ammonia oxidizing bacteria and biochar are mixed evenly in a weight ratio of 1:3, and ventilated and dried at room temperature until the water content is below 20% to obtain ammonia oxidation. Bacteria complex agent, packaged and stored in a cool and dry place.

[0023] (4) When starting a new ammonia nitrogen wastewater treatment A / O biore...

Embodiment 3

[0024] Embodiment 3: Ammonia oxidizing bacteria composite bacterial agent is used for removing ammonia nitrogen in polluted surface water

[0025] (1) Adjust the process conditions of ammonia nitrogen wastewater treatment A / O bioreactor to achieve efficient ammonia nitrogen removal and maintain stable operation.

[0026] (2) Without reducing the treatment efficiency of the bioreactor, 3% of the activated sludge returned to the sludge sedimentation tank is drawn out of the system to obtain a composite microbial culture rich in ammonia-oxidizing bacteria.

[0027] (3) With biochar passed through a 30-mesh sieve as a carrier, the composite microbial culture of ammonia oxidizing bacteria and biochar are mixed evenly in a weight ratio of 1:3, and ventilated and dried at room temperature until the water content is below 20% to obtain ammonia oxidation. Bacteria complex agent, packaged and stored in a cool and dry place.

[0028] (4) Adding 0.5% by weight of the ammonia oxidizing ba...

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Abstract

The invention relates to a preparation method of a composite bacterial agent of ammonia oxidation bacteria for removing ammonia nitrogen in sewage, and an application thereof, and belongs to the field of environmental biotechnology. The method mainly adopts activated sludge of a bioreactor for ammonia nitrogen waste water treatment as a main bacterial source to prepare the composite bacterial agent. The preparation method of the bacterial agent comprises the following steps: (1) adjusting the bioreactor for ammonia nitrogen waste water treatment to reach a process condition for high-efficient ammonia nitrogen removal, maintaining stable operation; (2) extracting part of sludge from activated sludge backflowed in a sludge sedimentation tank to obtain a composite microbial culture rich in ammonia oxidation bacteria; (3) mixing the composite microbial culture with biochar or zeolite powder carriers to prepare the bacterial agent; (4) wherein the bacterial agent is used for starting of a waste water treatment bioreactor, or enhancement of the removal efficiency of ammonia nitrogen in sewage. The composite bacterial agent of ammonia oxidation bacteria produced by the method has the characteristics of low production cost and good treatment effect, and reduces sludge discharge.

Description

Technical field: [0001] The invention belongs to the field of environmental biology technology, and relates to a preparation method and application of an ammonia oxidizing bacteria composite bacterial agent for removing ammonia nitrogen in sewage. Background technique: [0002] Ammonia nitrogen refers to the free ammonia (NH 3 ) and ammonium ions (NH 4 + ) in the form of nitrogen, and its content is an important indicator for measuring water quality and controlling sewage discharge. Its harm to the aquatic ecological environment is manifested in many aspects. Similar to COD, ammonia nitrogen is also the main oxygen-consuming pollutant in water bodies. The oxidation and decomposition of ammonia nitrogen consumes dissolved oxygen in water, making the water black and smelly. The non-ionic ammonia in ammonia nitrogen is the main factor that causes aquatic organisms to be poisoned. It is highly toxic to aquatic organisms, and its toxicity is dozens of times greater than that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C02F3/34C02F101/16
Inventor 白志辉赵志瑞李滨陈月月马安周
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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