Method for promoting acetonic acid excess accumulation by adding proline

Abstract

The invention relates to a method of boosting excessive accumulation of pyruvate by adding praline, belonging to directionally changing and optimizing microorganism metabolic function and optimizing fermentation process technical field, which is characterized in that: the torulopsis glabrata strain (T.glabrata) CCTCC M202019 of multiplex vitamin drawback is used as the starting strain, when the concentration of the sodium pyruvate is 0.4mol / L, praline is added into the culture medium and the concentration of the praline in the culture medium is 1g / L, the praline protects the torulopsis glabrata strain cells as compatible material and boosts the CCTCC M202019 to synthesize pyruvate. The method of boosting excessive accumulation of pyruvate by adding praline has the advantages that: the pyruvate fermentation cycle is shortened for 8 hours by adding 1g / L praline into the culture medium, and the pyruvate yield and production intensity is respectively enhanced for 5.4 percent and 18.6 percent; the research result can be applied to the organic acid fermentation and other typical industrial biological process, and provides new technique to industrial biotechnique, in particular the optimization of the fermentation process.

Description

technical field [0001] A method for adding proline to promote excessive accumulation of pyruvate belongs to the technical field of directional change and optimization of microbial metabolic function and optimization of fermentation process. technical background [0002] Pyruvate is an important precursor of various amino acids, vitamins and other useful substances. It has a wide range of uses in the chemical, pharmaceutical and agricultural chemical industries. Due to its high price, its popularization and application is limited. [0003] Pyruvate is a very important organic chemical intermediate, which is mainly used in medicine, pesticide and daily chemical industry. In recent years, the development and application of pyruvate has become a research hotspot at home and abroad. Pyruvate is used in the pharmaceutical industry to synthesize the new antihypertensive drug Angiotensin II, a series of protease inhibitors, sedatives, anti-inflammatory pain drug Xinkefen, anti-tuber...

AI Technical Summary

Problems solved by technology

[0004] During the fermentation process of pyruvic acid, in order to maintain the pH of the fermentation system in the optimum range, a certain amount of alkaline substances such as NaOH was added, and with the addition of NaOH, the osmotic pressure of the fermentation broth continued to increase, resulting in cell apoptosis and the decline in the ability to accumulate pyruvate, which ultimately affects the production efficiency of organic acids