Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi regulation

A technology of glutamic acid stick and glyoxylic acid, applied in the field of bioengineering, can solve few problems and achieve the effect of reducing the generation of by-products

Active Publication Date: 2021-07-20
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]It is very interesting for researchers to use CRISPRi technology to regulate the synthesis of glyoxylic acid, so as to help realize the industrial production of glyoxylic acid by biological methods Significance, but there are few reports about the production of glyoxylic acid by fermentation of genetically engineered bacteria in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi regulation
  • Method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi regulation
  • Method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi regulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0050] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0051] The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0052] This example relates to a method for synthesizing glyoxylic acid using Corynebacterium glutamicum based on CRISPRi regulation.

[0053] Corynebacterium glutamicum ATCC 13032 (C. glutamicum ATCC 13032 for short), as a food-safe microorganism, has the advantages of easy cultivation, no spore production, high yield, and biosafety. With the completion of the whole genome sequencing of Corynebacterium glutamicum and the accumulation of various omics data, the directional transformation and construction of engineering strains have been realized. During the glycolytic pathway and TCA cycle of C. glutamicum, pyruvate, lactate There is a complex network system among citric acid, isocitric...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi (clustered regularly interspaced short palindromic repeats i) regulation. According to the method, an acetaldehyde dehydrogenase coding gene of the corynebacterium glutamicum is replaced through a dCas9 expression cassette gene; a lactic dehydrogenase encoding gene of corynebacterium glutamicum is replaced by sgRNA transcription units of an isocitrate dehydrogenase encoding gene and a malic acid synthetase encoding gene, an isocitrate lyase encoding gene of the corynebacterium glutamicum is over-expressed to obtain a corynebacterium glutamicum engineering strain, and the engineering strain is cultured to ferment and synthesize glyoxylic acid. Corynebacterium glutamicum is used as a starting strain, a lactic dehydrogenase coding gene and an acetaldehyde dehydrogenase coding gene which are key enzymes of branch metabolism of the corynebacterium glutamicum are knocked out, a CRISPRi regulation system is established, expression levels of an isocitrate dehydrogenase coding gene and a malic acid synthase coding gene are reduced, and meanwhile, an isocitrate lyase coding gene is overexpressed, so that a method for biosynthesizing glyoxylic acid by using corynebacterium glutamicum is established.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for synthesizing glyoxylic acid using Corynebacterium glutamicum based on CRISPRi regulation for glyoxylic acid biosynthesis. Background technique [0002] Glyoxylic acid (Glyoxylic acid) is the substance with the smallest molecular weight in the alkydric acid family. It is an important organic acid in the TCA cycle (tricarboxylic acid cycle) and glyoxylic acid cycle of organisms, which affects the metabolic balance of organisms. Its derivatives There are dozens of kinds, among which the typical ones are vanillin, ethyl vanillin, allantoin, p-hydroxyphenylalanine, p-hydroxyphenylacetamide and so on. Glyoxylic acid derivatives are widely used in food additives, cakes, candy, baked food, tobacco and alcohol, spices, cosmetics and pharmaceutical industries. With the increase in demand for glyoxylic acid, the chemical production of glyoxylic acid brings Pollution is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12N15/77C12N15/66C12P7/40C12R1/15
CPCC12N15/102C12N15/77C12N15/66C12P7/40
Inventor 冯惠勇李天明王丽丽
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com