An antagonistic bacteria z-18 for controlling cotton verticillium wilt and its application

A technology of Z-18 and Bacillus, applied in the field of endophytes, can solve the problems such as unseen cotton endophytes, and achieve the effects of good control effect, improved yield and quality, and simple cultivation conditions.

Active Publication Date: 2021-06-08
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researchers have isolated endophytic bacteria from rice, tobacco, pepper, tomato, potato and other crops that have a good control effect on the host plant Verticillium wilt, but so far no cotton endophytes have been used for cotton Verticillium wilt organisms. Research reports on prevention and treatment

Method used

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  • An antagonistic bacteria z-18 for controlling cotton verticillium wilt and its application
  • An antagonistic bacteria z-18 for controlling cotton verticillium wilt and its application
  • An antagonistic bacteria z-18 for controlling cotton verticillium wilt and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation, screening and identification of Bacillus zhangzhouensis Z-18CGMCC No.15006

[0023] (1) Isolation and screening of strains

[0024] Several strains of bacteria were isolated from cotton tissue by gradient dilution method, and the plate confrontation method was used to judge whether they had antagonistic effect according to the presence or absence of inhibition zone, and the strains with certain antagonistic ability to the pathogen of cotton verticillium dahliat were preliminarily screened out. Further experiments were carried out on the strains with antagonistic effect, and the strains with stronger antagonistic ability were screened out for subsequent experiments.

[0025] A batch of microbial strains of endophytic bacteria were obtained through isolation, screening and cultivation in cotton tissue, and a strain numbered Z-18 was screened out, which belonged to Bacillus zhangzhouensis of the genus Microbacterium through microbiological classificat...

Embodiment 2

[0041] Example 2: Growth Factors of Bacillus zhangzhouensis Z-18 CGMCC No.15006

[0042] (1) According to the characteristics of Bacillus zhangzhouensis Z-18 CGMCC No.15006 provided by the present invention, its specific growth factors were determined, and the strain Z-18 was inoculated and cultured, and the results are shown in Table 2.

[0043] Table 2: Effects of temperature, pH, salt, antibiotics on the growth of strain Z-18

[0044]

[0045]

[0046] (2) Growth curve and culture conditions

[0047] The suitable pH value of strain Z-18 was screened and optimized, and the results are shown in Table 3:

[0048] Table 3: pH optimization results

[0049] 6.0 6.5 7.0* 7.4* 7.5 8.0 Z-18 0.522 0.794 0.864 0.928 0.612 0.001

[0050] Cultivate Bacillus zhangzhouensis Z-18 CGMCC No.15006 according to the method (1). The culture conditions of the bacteria are: the culture medium is TSA solid medium, and the culture conditions are: pH7.4, tempera...

Embodiment 3

[0052] Embodiment three: the fermentation process research of Bacillus zhangzhouensis Z-18 CGMCC No.15006

[0053] The purpose of obtaining preparations by fermentation is achieved through the optimization of carbon, nitrogen sources, pH value, etc.

[0054] (1) Determination of medium pH

[0055] According to the pH value of 6.0, 6.5, 7.0, 7.5, 8.0, ferment at 200r / min and temperature of 35°C, the optimum growth pH value is 7.0.

[0056] (2) Optimization of carbon and nitrogen sources

[0057] Seed liquid medium: glucose 2.0%, yeast extract 0.5%, corn steep liquor 7.5%, KH 2 PO 4 0.1%, K 2 HPO 4 0.1%, MgSO4 7H 2 O0.05%, continue to optimize on the basis of seed culture medium.

[0058] A. Carbon source optimization:

[0059] The carbon sources are glucose, sucrose, and corn steep liquor, and the other components are KH 2 PO 4 0.1%, K 2 HPO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, pH7.0;

[0060] B. Nitrogen source optimization:

[0061] Nitrogen sources were peptone, yea...

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Abstract

The invention discloses an antagonistic bacteria Z‑18 for preventing and treating Verticillium wilt of cotton and its application. Based on the relationship between plant endophytes and plant symbionts, a batch of endophyte strains are isolated from cotton, and a strain of genetic Stable characteristics, good biocontrol Bacillus ( Bacillus zhangzhouensis ) Z-18 CGMCC No.15006, when the screened endophytic strain Z-18 is used in the biological control of cotton Verticillium wilt, it can reduce the disease index of cotton Verticillium wilt, and the control effect is 59.5%. Cotton verticillium wilt, the main cotton disease, has a good control effect, and there is a significant difference (P<0.05) compared with the control, which becomes a new way to control cotton verticillium wilt, which is conducive to improving the yield and quality of cotton.

Description

technical field [0001] The invention relates to the technical field of microbial strains and applications thereof, in particular to the technical field of an endophyte and its application in the biological control of cotton verticillium wilt. [0002] technical background [0003] In the process of co-evolution with plants, a large number of microorganisms in plants have formed a special relationship with plants because they live in specific parts of plants for a long time. These microorganisms reproduce and spread in large numbers in plants. potential microorganisms. Endophytic bacteria are the microorganisms with the largest variety and quantity in plants. They have the advantages of wide distribution in plants, strong colonization ability, good disease control effect, and fast proliferation and diffusion. Anti-bacterial. [0004] Endophytes generally exist in plants, form a harmonious symbiont with the host, and produce a large number of secondary metabolic active substa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 史应武杨红梅楚敏林青曾军高雁霍向东牛新湘李玉国欧提库尔娄恺刘海洋
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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