Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale

The technology of agaric mold type II and Dendrobium officinale, which is applied in the field of microorganisms, can solve the problems of artificial planting, such as slow growth rate, low average yield, occurrence of diseases, etc., and achieves the effects of good development and application prospects, easy industrial production, and simple cultivation conditions.

Active Publication Date: 2014-12-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

Because orchid plants and fungi have a natural symbiotic relationship, the participation of fungi is not required from the germination of its seeds to the healthy growth of seedlings, which makes the artificial cultivation of Dendrobium officinale more difficult, and the growth rate of artificial planting is slow, the average yield is low, and disease more serious

Method used

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  • Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale
  • Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale
  • Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale

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Embodiment 1

[0023] The mycorrhizal fungus Umbelopsis dimorpha JSNL001-2 was prepared into a solid inoculum. The specific preparation method is as follows: using cottonseed shell fungus residue as a culture substrate, ammonium nitrate as an exogenous nitrogen source, and sucrose as an exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag at 121°C and 101.3 kPa Sterilize under high pressure for 20 minutes. After the culture medium is cooled, the mycorrhizal fungus JSNL001-2 is inoculated under aseptic conditions, and then cultured manually. The specific culture conditions were 28°C, 12h light + 12h darkness, pH 6.0, and culture for 30 days. Process the cultured bacteria into 1cm 3 Large and small masses are placed on the surface of the cultivation substrate for Dendrobium officinale seedlings planted that year. For the cultivation of Dendrobium officinale, a 15cm-diameter nutrient pot is used, and each nutrient pot is used to cultivate 1 cluster (5 plant...

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Abstract

The invention discloses umbelopsis dimorpha. The umbelopsis dimorpha belongs to umbelopsis genus of mucorale order of zygomycete subdivision; the strain number of the umbelopsis dimorpha is JSNL001-2, the umbelopsis dimorpha is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC No.9546, and the preservation date is August 15th, 2014. The umbelopsis dimorpha JSNL001-2 has remarkable effects in increasing the growth speed and improving the drought resistance of dendrobium officinale, can be cultured artificially, is high in reproduction speed, is convenient in on-scale production and has good development and application prospects.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a type II Agaricus and its application in the growth promotion and drought resistance of Dendrobium candidum. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of the genus Dendrobium of Orchidaceae. It is mainly distributed in the Southwest and Jiangnan provinces. It has immune regulation and anti-aging effects. It is an extremely rare and precious Chinese medicinal material recorded in the Chinese Pharmacopoeia. It is a part of Chinese patent medicines and health products. The essential raw material is known as "Chinese Immortal Grass" and "Gold in Medicine". Because orchid plants and fungi have a natural symbiosis relationship, from their seed germination to the healthy growth of seedlings, the participation of fungi is not required. This makes artificial cultivation of Dendrobium officinale more difficult, and artificial planting g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
Inventor 徐超张红岩陈露何任红郑凯赵桂华杨鹤同席刚俊史俊
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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