Method for breeding leaf mustard type rape varieties and materials by rape double haploid inducing line
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A mustard-type rape and double haploid technology, applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., can solve the problems of low induction efficiency, achieve high induction efficiency, improve breeding efficiency, and save breeding time Effect
Active Publication Date: 2016-10-26
CHENGDU ACAD OF AGRI & FORESTRY SCI
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The earliest maize haploid induction line is stock6, which can only induce haploids in maize, and then the haploid plants undergo artificial chromosome doubling to form homozygous...
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Embodiment 1
[0080] see figure 1 , figure 2 , Figure 5 , Figure 7 , Brassica napus GW (a local variety in the mountainous area of Sichuan, tetraploid, 2n=36) has the characteristics of yellow seeds, disease resistance, lodging resistance, high oil content (45%), compact plants, and poor soil resistance. High (above 2.5 meters) and late maturity; another mustard type rape broom mustard SBJ (Chengdu Jintang local variety, tetraploid, 2n=36) has a short growth period, early maturity, many branches, and a short plant height ( 150cm), resistance to poor soil and other characteristics, but the content rate is low (35-38%), brown kernels, slender, fluffy and not compact. In order to select a mustard rape line with better plant type, moderate plant height (160-170cm), yellow grain, short growth period, early maturity, high oil content, and poor soil tolerance, the above two local varieties were emasculated and crossed. 1 At the flowering stage, the buds were stripped and emasculated, and...
Embodiment 2
[0159] see figure 1 , figure 2 , Figure 4 , Figure 8 , Purple mustard type rape ZJ, the leaves are purple (with strong ornamental), but the growth period is long, the plant height is about 240CM, the seed coat is brown, and the production is poor in addition to ornamental yield traits. Mustard type rape broom mustard SBJ (Chengdu Jintang local variety, tetraploid, 2n=36) has the characteristics of short growth period, early maturity, many branches, short plant height (about 150cm), and poor soil resistance. Agronomic and economic traits. In order to select a mustard rape line with better plant type, moderate plant height (160-180cm), short growth period, early maturity, and tolerance to poor soil and purple leaves, the above two local varieties were emasculated and crossed with F. 1 At the flowering stage, the buds were stripped and emasculated, and the double haploid induction line Y3560 of rapeseed obtained by the applicant was used as the male parent for pollination...
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Abstract
The invention discloses a method for breeding leaf mustard type rape varieties and materials by a rape double haploid inducing line. The method comprises the following steps: firstly, determining objective traits of the bred leaf mustard type rape varieties and materials; secondly, performing hybridization, convergent cross or backcross on at least two leaf mustard type rapes with the objective traits; thirdly, pollinating progenies obtained by hybridization, convergent cross or backcross by using the rape double haploid inducing line; fourthly, identifying the genetic stability of induced progenies, and realizing selfing breeding according to strains; fifthly, identifying the yield, resistance and quality traits of induced stable progeny strains; sixthly, determining the stable strains according to the yield, the quality traits and the resistance to form a conventional variety of leaf mustard type rape; seventhly, inducing stable progeny genetic strains to form a new leaf mustard type rape line and performing test cross on the new leaf mustard type rape line and a leaf mustard type rape sterile line to form a maintainer line or restorer line, or entering next round of breeding process of the varieties and materials. According to the method, the breeding speed and efficiency of a hybrid variety or a conventional variety of the leaf mustard type rape can be greatly improved, and labor force and material resources are reduced.
Description
Technical field: [0001] The present invention is related to agriculture, in particular to a method for breeding mustard type rape varieties and materials with a double haploid induction line of rape. Background technique: [0002] Rapeseed is the main oil crop in my country, including 3 cultivars, Brassica napus ( Brassica napus , Brassica (aa, n=10) and Brassica oleracea (cc, n=9) are a compound species that evolved from double-diploidization after natural interspecific hybridization, and it is judged to be tetraploid according to the source of chromosomes, 2n=38 ); cabbage-type rape ( Brassia campestris L. Including brassica and cabbage originating in China. In China, it is also called cabbage, dwarf rape, and sweet rape. The chromosome group is aa, n=10, and it is diploid according to the source chromosome source, 2n=20,); Brassica juncea (Brassica juncea,, from Brassica (aa, n=10) and black mustard (bb, n=8) A compound species evolved by double-diploidization after ...
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