Application of gene encoding osbhlh116 protein, recombinant vector and recombinant bacteria in regulating rice plant type

A technology of osbhlh116 and rice plant type, applied in the field of genetic engineering, can solve the problem that the role of the highly expressed OsbHLH116 gene has not been further studied.

Active Publication Date: 2022-02-15
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it did not further study the role of highly expressed OsbHLH116 gene in rice

Method used

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  • Application of gene encoding osbhlh116 protein, recombinant vector and recombinant bacteria in regulating rice plant type
  • Application of gene encoding osbhlh116 protein, recombinant vector and recombinant bacteria in regulating rice plant type
  • Application of gene encoding osbhlh116 protein, recombinant vector and recombinant bacteria in regulating rice plant type

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] In the application of the gene encoding the OsbHLH116 protein in this embodiment in regulating rice plant type, the OsbHLH116 protein has the amino acid sequence shown in SEQ ID NO.2, and the gene encoding the OsbHLH116 protein has the amino acid sequence shown in SEQ ID NO.1 the nucleotide sequence.

Embodiment 2

[0045] In the application of the gene encoding OsbHLH116 protein in this embodiment in regulating rice plant type, the OsbHLH116 protein has the amino acid sequence shown in SEQ ID NO.2, and the gene encoding OsbHLH116 protein has the amino acid sequence shown in SEQ ID NO.3 The nucleotide sequence of the optimized gene for OsbHLH116. The acquisition process is as follows: through the rice genome annotation database Rice Genome Annotation Project http: / / rice.plantbiology.msu.edu / ) to find the OsbHLH116 gene (LOC_Os12g40730), its nucleotide sequence is SEQ ID NO.1, the encoded amino acid sequence It is SEQ ID NO.2. According to the enzyme cleavage site of vector pTCK303, the enzyme cleavage sites KpnI and SacI are required to construct the OsbHLH116-OX expression vector, and the SacI enzyme cleavage site is found in the coding sequence of the OsbHLH116 gene through sequence search. Therefore, according to the rice codon preference, in Under the premise of ensuring that the enc...

Embodiment 3

[0047] In this example, the application of the gene encoding OsbHLH116 protein in the regulation of rice plant type, the OsbHLH116 protein is the sequence of "KLDYKDHDGDYKDHDIDYKDDDDK" added after the amino acid sequence shown in SEQ ID NO. The dot-coded junction sequence, "DYKDHDGDYKDHDIDYKDDDDK" is a 3×FLAG tag sequence. The OsbHLH116 protein in this example is an OsbHLH116-FLAG fusion protein. The gene encoding OsbHLH116 protein has the nucleotide sequence shown in SEQ ID NO.4.

[0048] In other embodiments, after the amino acid sequence shown in SEQ ID NO.2, other types of tag proteins can be added to achieve the same technical effect.

[0049] Example 1 of the Application of the Recombinant Vector Containing the Gene Encoding the OsbHLH116 Protein in Regulating Rice Plant Type

[0050] In this example, the application of the recombinant vector containing the gene encoding OsbHLH116 protein in regulating rice plant type, the gene encoding OsbHLH116 protein has the nucleo...

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Abstract

The invention relates to the application of a gene encoding OsbHLH116 protein, a recombinant carrier and a recombinant bacterium in regulating rice plant type, and belongs to the technical field of genetic engineering. The present invention is for rice OsbHLH116 The gene sequence was optimized to obtain no Sac I restriction site OsbHLH116 Optimize the gene and connect it to the pTCK303 vector to obtain OsbHLH116 ‑OX expression vector, using the Agrobacterium-mediated method to transform the vector into the rice variety Nipponbare, and screen to obtain transgenic positive plants. In the experiment, it was found that compared with the wild-type Nipponbare, the plant height and leaf angle of the transgenic line at mature stage were significantly reduced, and the plant type was compact, indicating that OsbHLH116 Genes involved in the regulation of rice plant height and leaf angle in rice plant architecture. Therefore, this gene plays an important role in the establishment of rice plant type, and has potential application value in shaping and cultivating new rice varieties with ideal plant type.

Description

technical field [0001] The invention relates to the application of a gene encoding OsbHLH116 protein, a recombinant carrier and a recombinant bacterium in regulating rice plant type, and belongs to the technical field of genetic engineering. Background technique [0002] Rice (Oryza sativa L.) is one of the most important food crops in the world, and more than half of the world's population uses rice as a staple food. As the world population continues to grow and the area of ​​arable land continues to decrease, the problem of food security has become increasingly prominent. Therefore, cultivating new varieties of high-yielding, stable-yielding, high-quality and disease-resistant rice and increasing rice production are of great strategic significance for maintaining and ensuring my country's food security. [0003] Rice plant type refers to the morphological characteristics and spatial arrangement of rice. Rice plant type is one of the main factors determining rice yield, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC12N15/8261C07K14/415C12N2800/22
Inventor 杜彦修季新赵全志晏云李飞陈会杰孙红正张静李俊周彭廷
Owner HENAN AGRICULTURAL UNIVERSITY
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