82results about How to "Achieve identification" patented technology

The invention discloses a thin optical system, an image acquisition device and an electronic device. The thin optical system comprises a first lens, a second lens and a third lens with refractive power sequentially from an object side to an image side, wherein the first lens has negative refractive power, and the image side surface is a concave surface near an optical axis; the second lens has refractive power, at least one surface is an aspheric surface, and the second lens is made of plastic; the third lens has positive refractive power, at least one surface is an aspheric surface, and the third lens is made of plastic. Three lenses with refractive power exist in the thin optical system. The thin optical system further comprises an aperture arranged between the first lens and the second lens. The first lens, the second lens and the third lens have no mutual relative movement on the optical axis. When a specific condition is met, the thin optical system can be designed to be retrofocus, thereby enhancing telecentric effects. The invention also discloses an image acquisition device provided with the above thin optical system and the electronic device provided with the image acquisition device.

The present invention discloses a cultural relics identification system and method; modern techniques are applied to forensics of physical evidences for cultural relics, photoelectric image acquisition, parameter extraction, judging and comparison and evaluation output units are organically integrated, integrated technical innovation on track collection, evidence obtaining, comparison, verification and identification is achieved, and multi-step comprehensive identification on time and authenticity is provided for cultural relics. Natural aging traces of cultural relics, primary traces and secondary traces, can be recognized and identified, and qualitative and quantitative analysis is made for elements of cultural relics. Identification results provided by the system and method for the forensics process of physical evidences for cultural relics may directly proof that the objectivity of disputes, lay basis for the capitalization of cultural relics, and provide accurate and scientific methods for the identification of cultural relics.

The invention discloses a Cpf1 reagent kit for quickly detecting nucleic acid of an African swine fever virus. The Cpf1 reagent kit comprises a Cpf1 detection system suitable for quickly detecting theAfrican swine fever virus, and an immune colloidal gold test strip, wherein the Cpf1 detection system comprises specific crRNA protein, specific Cpf1 protein and a single-chain DNA(ssDNA) reporting system in accordance with a p72 gene of the African swine fever virus, the specific crRNA is one or more of crRNAs from ASFV P72 crRNA1 to ASFV P72 crRNA10, and the sequence of the specific crRNA is SEQ NO.4 to SEQ NO.13; and the single-chain DNA(ssDNA) reporting system comprises ssDNA FQreporter for fluorescence detection of a microplate reader and / or ssDNA DB reporter for detecting the immune colloidal gold test strip. According to the Cpf1 reagent kit disclosed by the invention, for the first time, the Cpf1 is used for detecting the African swine fever virus, and has the advantages of beinghigh in sensitivity, high in specificity, short in time consumption, high in flux, independent of large-scale experiment equipment and the like. The advantages enable a detection method based on the immune colloidal gold test strip developed by the invention to be conveniently used in basic laboratories and breeding enterprises to be used for performing detection, identification and diagnosis on basic quick detection of the African swine fever.

The present invention relates to an identification method for an O-glycosylation peptide fragment and a complete saccharide chain thereof. The technical process specifically comprises that a glycosylation protein sample is subjected to PNGase F digestion to remove N-glycosylation modification interference, enrichment is performed through hydrophilic interaction chromatography, and finally analysis is performed through time of flight mass spectrometer. According to the present invention, the method process has advantages of simple and rapid operation, high identification sensitivity, and the like; and the O-glycosylation peptide fragment and the complete saccharide chain thereof of the complex biological sample can be identified so as to achieve the research on the micro-uniformity of the O-glycosylation modification structure.

The invention discloses a molecular marker closely linked with the major QTL of wheat height and uppermost internode length as well as an acquisition method and application of the molecular marker. The acquisition method comprises the following steps: carrying out PCR amplification on DNA of a wheat variety Kenong9204 by adopting a marked primer Xcnl10; carrying out electrophoretic separation on the amplified product on 6.0% native polyacrylamide gel to obtain the amplified product with molecular weight of 700bp, namely the molecular marker of major QTL of wheat height and uppermost internode length. The molecular marker can be used for detecting whether a wheat variety or line contains QTL capable of reducing plant height and uppermost internode length, or not, so that the wheat variety or line containing QTL capable of reducing the plant height and uppermost internode length can be quickly screened for breeding, and the breeding progress for excellent wheat plant variety can be greatly accelerated.

The invention relates to a high performance liquid chromatography based illegal cooking oil cluster analysis method. Main components of a target oil sample comprise triglyceride and other impurities, and the analytic detection of illegal cooking oil is realized through the cluster determination of high performance liquid chromatography peaks. The method is mainly characterized in that a high performance liquid chromatography technology is adopted and fourteen characteristic chromatographic peaks are selected to obtain the area percentages of the peaks, and the area percentages are normalized; a cluster analysis technology is adopted to cluster the known oil sample to six types and establish a sample database; and data of an unknown sample are detected through discriminant analysis. The detection method has the advantages of simple sample pretreatment, high accuracy, and good repeatability, and can effectively avoid the false negativity appearing in the single impurity component detection because a plurality of fingerprints are adopted for identifying.

The invention discloses a three-piece type thin imaging lens group. The three-piece type thin imaging lens group sequentially comprises, from an object side to an image side: a flat plate element, which is made of glass; a first lens, which has negative refractive power and has a concave face close to an optical axis on an object side surface; an aperture; a second lens, which has positive refractive power and has a convex face close to an optical axis on an image side surface; and a third lens, which has positive refractive power. Therefore, large-angle light can be effectively collected, thethree-piece type thin imaging lens group can receive images in a larger range within an extremely short object distance and achieve an identification effect, the distance between a shot object and the three-piece type thin imaging lens group can be shortened, the size can be effectively reduced, and miniaturization of the three-piece type thin imaging lens group can be maintained.

The invention discloses molecular markers of major QTL for the rice heading period and application of the molecular marker.The molecular markers include an H70 primer pair and a 301K primer pair, wherein the H70 primer pair is composed of a sequence shown as SEQ ID No.1 and a sequence shown as SEQ ID No.2; the 301K primer pair is composed of a sequence shown as SEQ ID No.3 and a sequence shown as SEQ ID No.4; the two molecular markers are closely linked with QTL for the heading period and can be used for authenticating and breeding varieties for the rice heading period.The invention further discloses a method for authenticating and breeding the varieties for the rice heading period through the molecular markers.The major QTL which are located on the galianconism of the fifth chromosome and used for controlling the heading period are precisely positioned for the first time, and the InDel markers H70 and 301K which are closely linked are obtained.Whether variation of the major QTL for the heading period exists or not can be judged and authenticated just by detecting the characteristics of amplified bands of the markers, the phenotype of rice in the heading period can be predicted, and the molecular markers are used for guiding breeding work for an improvement in the rice heading period.

The invention relates to a roller drive plate clamping tool. The roller drive plate clamping tool comprises a disc-shaped laminar driver disk, the outer circumference of the driver disk is provided with a drive pawl which is matched with a shifting fork arranged on a roller grinder main shaft, a drive hole which is matched with a roller drive handle is arranged in the middle of a circular disc, one ends of a plurality of supporting rods are uniformly distributed and fixedly connected on the end face of one side of the driver disk, the other ends of the supporting rods penetrate through a disc-shaped laminar support disk to arrange limit end sockets, springs are sleeved on the supporting rods, a flexible connector is mounted between the support disk and each of the supporting rods, and the support disk is connected with the roller grinder main shaft through a bolt. According to the roller drive plate clamping tool, the support disk and the supporting rods are used for connecting the driver disk on a main shaft face plate of a roller grinder in advance, by means of the elastically extending and retracting of the supporting rods, the clamping of a roller is convenient, simultaneously, a flexible connecting mechanism is arranged on the supporting rods, the support disk and the driver disk can relatively twist, and the problem of mutual interference is solved. The clamping tool has the advantages that the structure is simple, the usage is convenient, the work efficiency is increased, and the remarkable economic benefit can be achieved.

The invention discloses a functional hyperspectral CT (computed tomography) imaging-based substance recognition method and a functional hyperspectral CT imaging-based substance recognition system. The system comprises an X-ray tube, a photon counting detector, a two-dimensional translation platform, a rotating platform, a three-dimensional motion controller and a control center, wherein the X-ray tube is connected with a high voltage generator to emit an X ray to scan a tested sample; the rotating platform is positioned in front of the emitting end of the X-ray tube to support the tested sample; the photon counting detector is positioned on the two-dimensional translation platform to acquire hyperspectral data; the three-dimensional motion controller is connected with the rotating platform to control the rotating platform to rotate, and is connected with the two-dimensional translation platform to control the two-dimensional translation platform to translate; the control center is connected with the three-dimensional motion controller, the photon counting detector and the high voltage generator to control the three-dimensional motion controller and the high voltage generator, receive the hyperspectral data and recognize the tested sample. The method and the system have a broad application prospect in disease diagnosis, food safety and nondestructive testing.

The invention discloses an identification method of rice geographical indications and an application of the identification method of the rice geographical indications, and belongs to the technical field of safety and quality detection on agricultural products. The identification method comprises the steps: performing crushing pretreating on a to-be-detected sample, acquiring feature smell information of the pretreated rice by using an electronic nose, establishing a discrimination model, and determining the rice geographical indications according to the discrimination model. The method in which electronic nose fingerprint analysis and multivariate statistical analysis are combined, provided by the invention, realizes the identification of the rice geographical indications, has the advantages of high detection accuracy rate, convenience in operation, rapidness, nondestructive detection and the like, the correct classifying rate of samples can be up to 90 percent, the tracing of a rice producing area is effectively performed, and the method is suitable for on-site rapid identification of the rice producing area.

The invention discloses a storage space reservation method based on a shared printer and a request receiving device. The method comprises the steps that a pre-printing request sent by a terminal is received; according to information carried in the pre-printing request, a storage space reservation request of the terminal is acquired; according to information carried in the storage space reservationrequest, reserved fetching time when a terminal user fetches a file requested to be printed is acquired; according to the information carried in the storage space reservation request, a storage spaceis allocated to a pre-printing file carried in the pre-printing request; and the pre-printing file is printed, and the printed pre-printing file is stored into the allocated storage space. The devicecomprises a request receiving unit, an information acquisition unit, printing equipment and a storage unit. The method is reasonable, and the device is simple in structure. Through the method and thedevice, automatic storage of the file printed by the user can be realized.

The invention provides a micro-fluidic chip applied to capture and screening of single cells, and an application thereof. The micro-fluidic chip comprises four layers of structures which are successively stacked together and mutually sealed and are respectively a micro-valve control layer, a micro-valve film layer, a substrate with a flow way and a micro-pore array and a detection release layer from top to bottom. Through introduction of a micro-processing technology and a micro-fluidic technology, the multi-layer and multi-functional micro-fluidic chip is developed, capture, identification, culture and release of cells are realized, a new method is provided for cell screening, and hyper-secretion cells can be obtained more quickly, accurately and conveniently.

The invention discloses a functional specific molecular marker PikFNP for resistance genes Pik of rice blast and a method and application of the functional specific molecular marker PikFNP. The molecular marker Pik-FNP is a nucleotide sequence which is amplified from total deoxyribonucleic acid (DNA) of rice by a primer pair SEQ ID NO. 1 and SEQ ID NO. 2. By a method for contrasting an allelic gene sequence of multiple resistance genes Pik of rice blast to a Pik-1 sequence and a Pik-2 sequence, Pik functional specific single nucleotide polymorphism (SNP) which can be different from susceptible allelic genes and other resistance genes of rice blast can be obtained through analysis, and a primer SEQ ID NO. 1 and a primer SEQ ID NO. 2 are obtained by design; and the DNA of rice is subjected to amplification and specific digestion so as to obtain the Pik functional specific molecular marker Pik-FNP. The Pik functional specific molecular marker Pik-FNP can be screened from a large number of rice genetic resources and can be applied to molecular marker-assisted selection breeding, gene aggregation breeding and transgenic breeding, and the functional resistance genes can be identified.

The invention discloses a molecular marker closely linked with wheatear grain number major QTL. Wheat genome DNA serves as a template, a PCR primer pair is adopted to conduct PCR amplification, the amplificated product is subjected to ionophortic separation through polyacrylamide gel to obtain the molecular marker 2A-654918338. The invention further discloses application of the molecular marker 2A-654918338 in detecting whether a wheat variety or line contains the QTL for increasing the wheatear grain number and application of the molecular marker 2A-654918338 in molecular breeding of wheat. By means of the molecular marker, the genetic improvement progress of the wheatear grain number is accelerated, the selection efficiency and quality of the wheat variety or line are greatly improved, identification of the target gene in wheat genetic resources and breeding progenies is directly achieved, and more new wheatear grain number genetic resources are developed for wheat breeding.

The invention relates to a method for preparing a hydrophilic hybrid integral material through a thiol-ene click chemical reaction. The method comprises mixing a modified polyhedral oligomeric silsesquioxane reagent, a sulfhydryl compound, an initiator and a pore-foaming agent, carrying out ultrasonic dissolution and preparing the organic-inorganic hydrophilic hybrid integral material through a thiol-ene click chemical reaction in situ under UV irradiation. The porous hybrid integral material has a uniform and regular microstructure and ordered pores and can be well used in chromatographic separation. The preparation method has the advantages of simple and quick operation, fast reaction speed and the like, and can also select different functional monomers and pore-foaming agent systems according to different application requirements to prepare a series of porous hybrid integral materials with different physical and chemical properties.

The invention discloses a functional specificity molecule marker Pi7FNP of blast-resistance gene Pi7, a method and an application thereof. The molecule marker consists of Pi7-1FNP and Pi7-2FNP, wherein Pi7-1FNP is the nucleotide sequence amplified from rice total DNA by SEQ ID NO.1 and SEQ ID NO.2, and Pi7-2FNP is the nucleotide sequence amplified from rice total DNA by SEQ ID NO.3 and SEQ ID NO.4. According to the invention, two single-base differences which can be different from the Pi7 functional specificity of the disease-susceptibility alleles and other blast-resistance genes are gained by the analysis through comparing allele sequences of the rice blast-resistance gene Pi7 with the sequences of SEQ ID NO.5 and SEQ ID NO.6; primers SEQ ID NO.1 and primers SEQ ID NO.2 and primers SEQ ID NO.3 and primers SEQ ID NO.4 are respectively obtained by designing; by carrying out DNA amplification and then specific enzyme digestion on rice, the Pi7-1FNP and Pi7-2FNP are respectively obtained. The invention can be applied in screening from large amounts of rice genetic resources and identifying the functional resistant genes, and molecular marker assisted selection, gene polymerization breeding and transgenic breeding.

The invention discloses a detection primer and probe for rapidly identifying siniperca chuatsi on the basis of a quantitative PCR technology, and a detection kit and detection method adopting the primer and the probe. A detection primer group comprises an upstream primer FP, a downstream primer BP and a probe; and the detection kit comprises a primer solution, a PCR MIX reaction solution, deionized water, a negative control and a positive control. The detection method comprises the following steps: extracting DNA of a sample to be detected, quickly detecting the DNA of the sample to be detected by using a fluorescent quantitative PCR technology, and judging whether the sample to be detected is siniperca chuatsi or not through a real-time amplification curve. The detection primer, the probe, the kit and the method provided by the invention have the advantages of rapidity, sensitivity, specificity, easy operation and the like, and are suitable for popularization and application.

The invention relates to the technical field of detection and amplification of unicellular antibodies, specifically to a micro-fluidic chip and application thereof. The chip consists of a micro-valve control layer, a micro-valve thin film layer, a cell treatment layer and a substrate layer which are sequentially stacked together and are mutually sealed; the chip has a liquid injection port, a cell suspension inlet, a cell suspension outlet, an amplification product collection outlet, a micro-valve air inlet, a micro-valve air inlet, an amplification product release micro-valve control structure and a liquid flow micro-valve control structure; the amplification product release micro-valve control structure controls release of amplification products through a micro-valve on a gas flow channel; the liquid flow micro-valve control structure controls the flow of liquid in the cell treatment layer through the micro-valve on the gas flow channel; the cell treatment structure comprises a plurality of cell treatment units; each cell treatment unit comprises a liquid flow channel and a gene amplification chamber; and capture, identification and amplification of cells are accomplished in the cell treatment units. According to the invention, all the functions are integrated on the same chip, and operation is quick, simple and convenient.

The invention provides a rapid classification and identification method for sea foods in a close genetic relationship and aims to classify and identify different sea foods in close species. The methodcomprises the following steps: taking a part of meat and skin of sea foods as samples to be tested; treating the samples to be tested in an alkali solution; adjusting the solution to be neutral or weak acid; desalting the solution; detecting the solution with a mass spectrum, and recording spectrum signals; carrying out chemometric analysis on the acquired spectrum signals, thereby distinguishingdifferent sea foods. The method provided by the invention is simple and feasible, is capable of achieving scientific identification on sea foods of a same specie within hours on the basis of sample pretreatment, and is good in resolution and high in sensitivity.

The invention relates to a group of specific primers for identifying tortoise shells and an identification method thereof, and belongs to the technical field of identification of traditional Chinese medicines. Firstly, genomic DNA is extracted and purified after tortoise shell products are crushed, the sample is used as a template, designed and screened specific nucleotide sequences are selected as primers for PCR amplification, then products are subjected to agarose gel electrophoresis analysis and gel imaging system detection. Finally, authenticity and adulteration of tortoise shell productsare judged based on test results. The identification method is easy to operate, does not require sequencing, has strong specificity, strong anti-interference ability, good reproducibility, has a widerange of application, and can be used for accurate identification of tortoise shell origins, medicinal materials and decoction pieces, and a practical method is provided for judging the authenticityand adulteration of the tortoise shell products.

The invention provides a method and system for identifying a Chinese population epithelial cell body fluid spot tissue source from the gene level. The method comprises the steps of utilizing a first PCR amplification primer set for performing first amplification on body fluid spot DNA, and obtaining a first amplification PCR product, wherein the first PCR amplification primer set is composed of nine pairs of amplification primers corresponding to nine genes of CYP2B7P1, MUC4, L.cri, HTN3, STATH, MUC7, LCE2B, DCD and CCL27 one to one; according to the gene expression condition in the first amplification PCR product, whether or not at least one of vaginal secretion, saliva and skin is contained in body fluid spots is judged. According to the scheme, the Chinese population body fluid tissue source can be effectively determined from the gene level, the step for identifying the body fluid or spot tissue source thereof at a criminal investigation site is greatly simplified, and the accuratescientific basis can be supplied to case character clearing, conviction and sentencing.

The invention provides a towed plankton imager and an imaging system. The towed plankton imager comprises an imaging cabin component, a light source cabin component, an optical support, and a power supply module. The light source cabin component comprises a second guide cover, a light source cabin body and a second rear cover, a second window disposed on one side of the light source cabin body is equipped with a second light transmission window, a light source unit installed in the light source cabin provides a lighting light source through a second light transmission window, the imaging cabin component consist of a first guide cover, an imaging cabin body and a first rear cover, a first window disposed on one side of the imaging cabin body is equipped with a first light transmission window, and an imaging unit disposed in the imaging cabin can acquire plankton image photos through the first light transmission window. The towed plankton imager provided by the invention can provide a lighted area through the light source cabin component, and acquire the image photos of plankton in the lighted area through the imaging cabin component, thus realizing identification of zooplankton, also the operation is convenient and the structure is simple.

The invention discloses a thin imaging lens group. The thin imaging lens group sequentially comprises a flat plate component, a first lens, a diaphragm and a second lens from an object side to an image side, wherein the flat plate component is made of a glass material, the first lens has negative refractive power, at least one of a surface of the object side and a surface of the image side of thefirst lens is a non-spherical surface, the second lens has positive refractive power, a convex surface is arranged at a position, near an optical axis, of a surface of the object side of the second lens, a convex surface is arranged a position, near the optical axis, of a surface of the image side of the second lens, and at least one of the surface of the object side and the surface of the image side of the second lens is a non-spherical surface. Therefore, a light ray at a large angle is effectively collected, an image in a larger range is received by the thin imaging lens group within an extremely short object distance, and the identification effect is achieved; and meanwhile, the distance between a shot object and the thin imaging lens group is favorably reduced, the size can be effectively reduced, and the miniaturization of the thin imaging lens group is maintained.

The invention discloses a method for detecting RNA m6A with high flux, high sensitivity and single base resolution and an application thereof. The method comprises the following steps: (1) extractingtotal RNA in a sample and removing rRNA to obtain an RNA sample; (2) fragmenting the RNA sample, and dividing the fragmented RNA sample into two parts, namely an RNA fragment I and an RNA fragment II;(3) carrying out co-immunoprecipitation and cross-linking treatment on the RNA fragment I; (4) respectively carrying out 3'tail end joint connection on the complex obtained by cross-linking treatmentand the RNA fragment II; (5) respectively carrying out reverse transcription to obtain cDNA, connecting the cDNA with a 3'end joint, carrying out PCR amplification, and respectively constructing an hmCLIP library and an input library; and (6) sequencing the hmCLIP library and the input library by using a high-throughput sequencing platform. The method is short in consumed time, high in sensitivity and suitable for detecting RNA m6A in a small number of specimens and specimens with low RNA integrity.

The invention discloses an ALK fusion gene detection and typing kit based on sandwich method high-resolution melting curve analysis. A sandwich sequence target segment is amplified in a segmented manner by adding a low GC content joint at the 5' end of a gene-specific primer, and is a fusion gene fragment comprising a high and low GC content consensus sequence and a variable sequence. A high-resolution melting curve general analysis method can judge presence or absence of the fusion gene, and the fluorescence intensity-temperature second-order derivative curve is combined with a key parameteranalysis method to achieve digital judgment of the fusion gene type. The kit solves the problems of high cost, long cycle and difficult typing of the existing lung cancer ALK fusion gene detection, integrates the advantages of two technologies of real-time quantitative reverse transcription PCR and high-resolution melting curve analysis, and can be applied to fusion gene detection of fresh tissues, paraffin-embedded tissues, pleural effusion and other types of specimens.

The invention discloses an automatic parking device usage information storing system and method and a vehicle. The system comprises a receiving device, a control device and a storage device, wherein the receiving device is used for receiving a signal indicating that an exit of an automatic parking device; under the circumstance that the exit of the automatic parking device is an abnormal exit of the automatic parking device, the control device is used for outputting the reason for the abnormal exit and the trip distance of the vehicle by the time of the abnormal exit; the storage device is used for storing the reason for the abnormal exit and the trip distance of the vehicle by the time of the abnormal exit. By using the automatic parking device usage information storing system, a parking accident can be identified conveniently.

The present invention discloses a method for identifying the N-linked oligosaccharide structure of novel erythropoiesis stimulating protein. The method comprises: (1) determining the linking way of the terminal sialic acid on the N-linked oligosaccharide structure of novel erythropoiesis stimulating protein by combining sialidase digestion and laser-induced fluorescence capillary electrophoresis separation; (2) attributing the desialylated N-linked oligosaccharide structure by combining exglycosidase digestion sequencing and laser-induced fluorescence capillary electrophoresis separation; and (3) analyzing the N-linked oligosaccharide structure by using laser-induced fluorescence capillary electrophoresis and the two-dimensional separation technology of weak anion exchange chromatography and combining sialidase digestion. According to the present invention, the method has characteristics of high sensitivity, high separation degree, simple operation, low cost and high accuracy, is suitable for high-throughput detection and industrial applications, and performs the structure identification on the saccharide chain accounting for 95% by mass of the N-linked oligosaccharide structure on the novel erythropoiesis stimulating protein.