ALK gene and EML4 gene detection probe, preparing method thereof and kit

A gene detection and detection probe technology, applied in the field of ALK gene and EML4 gene detection probes and their preparation, can solve the problems of high detection kits and lack of specificity, and achieve the effects of good repeatability and accurate signal counting
CN105543353AInactive Publication Date: 2016-05-04GUANGZHOU LBP MEDICINE SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU LBP MEDICINE SCI & TECH
Publication Date
2016-05-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an ALK gene detection probe, an ALK gene and EML4 gene detection probe and a preparing method thereof. The preparing method comprises the following steps that BAC aiming at an ALK gene is selected to be cloned into at least one of RP11-62B19, RP11-100C1 and CTD-2245E6, and BAC is selected to be cloned into at least one of CTD-2544I11, RP11-684O3 and RP11-134O13; BAC aiming at an EML4 gene is selected to be cloned into at least one of CTD-2358L8 and CTD-2547J15; plasmid DNA is obtained; marking is carried out. The invention further discloses a kit containing the ALK gene and EML4 gene detection probe. The optimal ALK gene and EML4 gene detection probe is obtained by means of screening, signal counting is accurate and fast, and result repeatability is good.
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Description

technical field

[0001] The invention belongs to biotechnology, and in particular relates to ALK gene and EML4 gene detection probes, preparation methods and kits thereof. Background technique

[0002] In 2014, the annual report of tumor registration indicated that lung cancer ranked first in the incidence rate (35.23 / 100,000, 19.59%) and death rate (27.93 / 100,000, 24.87%) of malignant tumors in China. The occurrence of lung cancer is considered to be a multi-step process, which is closely related to the amplification / activation of proto-oncogenes and / or the inactivation of tumor suppressor genes. opportunity for treatment. The study found that 64% of lung adenocarcinoma patients detected cancer drivers, of which 8% were ALK rearrangements. ALK gene fusion is not a favorable prognostic factor for advanced NSCLC, but crizotinib can improve the survival of ALK-positive patients. Previous studies have shown that the EML4-ALK gene fusion mutation rate is 2% to 7%. A study rep...

Claims

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