Rapid detection kit and method of NTRK gene fusion

A technology of gene fusion and kits, applied in biochemical equipment and methods, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as no diagnostic technology widely recognized, and achieve the effect of accurate diagnosis and auxiliary accurate stratification

Inactive Publication Date: 2019-08-20
GUANGZHOU LBP MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

There is currently no definitive diagnostic technique that is widely accepted

Method used

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  • Rapid detection kit and method of NTRK gene fusion
  • Rapid detection kit and method of NTRK gene fusion
  • Rapid detection kit and method of NTRK gene fusion

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Embodiment Construction

[0046] The present invention is further described below through specific examples, but the examples do not limit the scope of patent protection of the present invention in any way.

[0047] Embodiment A kind of NTRK gene fusion rapid detection reagent and kit thereof

[0048] 1. Probe Design

[0049] The reagent contains 3 probes, involving 3 segments on the genome, namely NTRK1, NTRK2 and NTRK3 genes. The design process includes:

[0050] BAC clone selection → purchase (commercialization) → labeling, testing → confirmation of the probe clone group (available for subsequent production).

[0051] For the method of BAC clone screening, please refer to p26-28 of Zhao Jia et al.

[0052] · BAC clone selection:

[0053]

[0054]

[0055] 2. Probe Preparation

[0056] Probe labeling (using phi29 DNA polymerase labeling) → quantification (#The amount and length of the probe will affect the hybridization effect) → packaging / stand-by.

[0057] Using the BAC clone as the basi...

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Abstract

The invention relates to a rapid detection method and kit of NTRK gene fusion and belongs to the field of molecular biological detection. According to the rapid detection kit of NTRK gene fusion, a probe is prepared through bought BAC cloning corresponding to the probe by use of phi29DNA polymerase labeling, a buffer solution component is determined by optimization, and the prepared kit can realize rapid hybridization within 2 h and accurate interpretation of NTRK gene rearrangement. The detection kit can better assist existing method for accurate layering, precise diagnosis and treatment of tumor through component optimization and detection of reagent commercialization and has better application prospects.

Description

technical field [0001] The invention relates to a kit for rapidly detecting NTRK gene fusion and a method thereof, belonging to the field of molecular biology detection. Background technique [0002] The NTRK genes include NTRK1, NTRK2 and NTRK3, which are responsible for the synthesis of the tropomyosin receptor kinase (TRK) family proteins TRKA, TRKB and TRKC, respectively. These proteins play an important role in the development of the nervous system. Binding of neurotrophic factors to TRK protein can induce receptor dimerization, phosphorylation and activate downstream signaling cascade pathways of PI3K, RAS / MAPK / ERK and PLC-γ. Changes in the TRK signaling pathway, including gene fusion, protein overexpression or single nucleotide changes, have been found to be the cause of many tumors, especially the fusion of the NTRK gene, which is currently the most definite cause of cancer. [0003] NTRK gene fusions occur in a variety of solid tumors in adults and children, includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 陈绍宇何瑰
Owner GUANGZHOU LBP MEDICINE SCI & TECH
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