61results about How to "The test result is clear" patented technology

The present invention relates to protein chip based on labeling streptavidin-biotin technology. The hybridization reaction includes the following steps: dropping tested sample to sample applied chip and washing out redundant sample with elution liquid; blocking wtih blockage liquid, washing and air drying; dropping protein marked with biotin diluted by blockage liquid, washing and air drying; anddropping fluorescnet or enzyme labeled streptavidin diluted by blockage liquid, washing and air drying. The protein chip of the present invention is used in determining antigen and antibody, and detecting specific matter combined with protein. The improved method of the present invention has clear, stable and reliable test results and wide application range.

The invention relates to a code protocol security detecting method based on freshness verification conditions in the field of information security, which comprises the steps that: inputting the initial value of a code protocol security detecting result, generating a new fact after a communication agent sends or receives each message, obtaining a new security detecting result through operating the freshness verification condition of a confidence multi-collection method based on the new fact and the built security detecting result until to process the protocol information, obtaining the security conclusion of the code protocol through the code protocol security detecting result, or displaying that the code protocol is safe, or leading out an attack structure for the code protocol with security hidden dangers. The detecting method not only can effectively differentiate whether the message is fresh, but also substantially prevents the attacks of replaying and confusing, and is not related to the specific descriptions of a multi-protocol operation environment and the ability of an attacker. In addition, the invention not only is a method of proving the code protocol correctness, but also is a method of searching protocol errors.

The invention relates to a detecting device for positive and negative surfaces of a valve seat ring. The detecting device comprises a vertical main bracket, wherein the upper part of the main bracket is fixed together with a cylinder barrel of an air cylinder; a piston rod of the air cylinder faces upwards and is fixedly connected with the right end of a bracket I; a detection rod penetrates through a longitudinal seam in the left end part of the bracket I; a detection head is arranged at the lower end of the detection rod; a spring in a compression state sleeves the detection rod between the detection head and the bracket I; a limiting gasket is fixed at the upper part of the detection rod; a clamping block fixed on the detection rod is arranged above the limiting gasket; a limiting slot which penetrates in the front-rear direction is formed in the right end of the clamping block; a bracket II is fixed on the middle part of the bracket I; a pin II in the front-rear direction is arranged at the left end of the bracket II; the pin II is rotationally connected with a light shielding plate; a pin I penetrates through the limiting slot and the left end part of the light shielding plate in the front-rear direction; a photoelectric switch is arranged at the right end of a bracket II; the right end of the light shielding plate is provided with a light through slot corresponding to the photoelectric switch. According to the scheme, the invention provides the detecting device, which adopts a mechanical contact mode and is high in reliability, for positive and negative surfaces of the valve seat ring.

The invention relates to a seahorse identification method, and belongs to the field of molecular biology. The technical problem to be solved is to provide a seahorse identification method. The seahorse identification method can realize the accurate identification of hippocampus japonicus, hippocampus trimaculatus and hippocampus kuda. The seahorse identification method comprises the following steps of: a. extracting seahorse DNA (deoxyribonucleic acid); b. carrying out PCR (Polymerase Chain Reaction) amplification on the seahorse DNA; and c. after ending the PCR amplification, adding 1 microlitre of 100X SYBR Green I dye to a reaction system, and detecting the color of the sample under an ultraviolet lamp; if the sample is not yellow or yellow green, the sample does not contain the corresponding seahorse, ending identification; and if the sample is yellow or yellow green, the sample contains the corresponding seahorse, taking 5 microlitre of PCR product to carry out 1.5% agarose gel electrophoresis detection, and identifying the seahorse variety.

The invention discloses a method for detecting gene mutation. The method comprises the following steps of: synthesizing a forward primer P1-F and a reverse primer P1-R of a long segment deficiency gene mutation sequence; synthesizing a forward primer P2-F and a reverse primer P2-R of a gene mutation sequence of the long segment deficiency gene mutation sequence; using a sequence to be detected as a template and performing a polymerase chain reaction (PCR) for one time by using a mixture of the four primers as a primer; and performing genotype analysis on the product of the PCR to determine the condition of the gene mutation. By the method of the invention, the long segment sequence deficiency mutation and the gene mutation thereof can be detected conveniently at the same time. By the method for detecting the gene mutation, the long segment sequence deficiency mutation can be detected more conveniently and stably without using expensive reagents such as linker adaptor (LA) amplification enzyme and the like; the cost of detecting the gene mutation is reduced; and the detection efficiency is improved.

The invention discloses a steel ball detection device comprising a baseplate. The two sides of the upper surface of the baseplate are fixedly connected with the bottom parts of a left plate and a right plate. The top parts of the left plate and the right plate are fixedly connected with the bottom part of connecting plates, and one side of each of the two connecting plates is fixedly connected with a clamping device. A vertical plate is fixedly connected on the middle part of the upper surface of the baseplate. A detection rod is sleeved by a fixing plate, and the bottom part of a top plate isflexibly connected with the upper surface of the fixing plate through a detection spring. A pointer is fixedly connected on the top end of the detection rod. The detection rod is improved, whether the steel ball is qualified is determined through the reading of the pointer on the scale plate, the steel ball can be clamped by improving the clamping device and detection of the steel ball is facilitated, and omnibearing detection can be performed under cooperation of the bearing so that the effect of stable detection can be realized.

The invention relates to the technical field of an industrial robot and discloses an industrial rail parallelism detection robot. The industrial rail parallelism detection robot comprises two rail bodies and a positioning plate. Detection slide blocks are fixedly connected with the bottom of the positioning plate. The detection slide blocks are in slide connection with the rail bodies. Drive wheelassemblies are fixedly mounted at the bottom of the positioning plate. The drive wheel assemblies are movably connected with the rail bodies. A slide slot is set on the top of the positioning plate.A motor is fixedly connected with the inner wall of the slide slot. A screw rod is fixedly connected with an output shaft of the motor. Screw rod slide bushings are in threaded connection with the surface of the screw rod. Moving plates are fixedly connected with the tops of the screw rod slide bushings. According to the industrial rail parallelism detection robot, the problem that most of the existing rail parallelism detection robots are integrated structures, when faults occur in the robots, the robots are relatively troublesome to disassemble, maintenance or replacement of parts is inconvenient, and after the maintenance is finished, assembly is relatively troublesome is solved.

The invention discloses a kit for detecting an HIV-1 p24 antigen. The kit comprises an enzyme linked immunosorbent assay (ELISA) plate, a sample diluent, an enzyme conjugate, a negative control substance, a positive control substance, a substrate solution, a developing solution, a stop solution and a cleaning solution, wherein the ELISA plate is coated with an anti-mouse HIV-1 p24 monoclonal coating antibody, and the anti-mouse HIV-1 p24 monoclonal coating antibody is a 2-C6 antibody; the sample diluent contains a biotin-labeled anti-mouse HIV-1 p24 monoclonal antibody, and the biotin-labeled anti-mouse HIV-1 p24 monoclonal antibody is a biotin-labeled 11-A4 antibody. In addition, the invention also discloses application of the kit. Experiments show that the kit is easy to operate and accurate and clear in detection result, and the antigen sensitivity reaches 1.25IU/mL and is remarkably higher than the detection sensitivity of a conventional ordinary HIV-1 p24 detection kit when the kit is used for detecting the HIV-1 p24 antigen.

The invention discloses a saliency detection method for cross-modal enhancement and improvement of a loss function, and the method comprises the steps: constructing a convolutional neural network at atraining stage, wherein an input layer comprises a color image input layer and a depth image input layer, wherein a hidden layer comprises a coding frame composed of a color coding stream, a depth coding stream and a coding feature extraction stream and a decoding frame composed of a decoding stream, and the output layer is composed of five sub-output layers; inputting the R, G and B channels ofthe color image in the training set and a three-channel depth map obtained by copying the corresponding depth image into a convolutional neural network for training; obtaining a verification weight vector and a verification bias term of the convolutional neural network training model through a loss function value between the saliency detection graph and the saliency real detection graph obtained through calculation; verifying the verification set to obtain an optimal weight vector and an optimal bias term; in the test stage, using the optimal weight vector and the optimal bias term for prediction to obtain a saliency prediction image; the invention has the advantage of high detection precision.

An oral endoscope comprises a handle, a lens frame connected to the handle, an endoscope body connected to the lens frame, a rotating mechanism connected between the lens frame and the handle, a rotating mechanism including a hinge seat connected to the handle, a hinge shaft matched with the hinge seat connected to the lens frame, and a driving mechanism connected to the hinge shaft for driving the hinge shaft to rotate. The oral endoscope realizes the convenient adjustment of the endoscope lens in the use process, The invention enlarges the detection range of the endoscope, makes the medicalpersonnel use the endoscope more conveniently in the detection process, and solves the problem that the lens is easy to fog due to the temperature difference between the oral cavity of the patient andthe external temperature of the patient, so that the detection result is clearer, and the medical personnel can analyze the condition of the patient more accurately.

The invention discloses a method for preparing O type foot-and-mouth disease virus-like particles and test paper, and relates to the technical field of serological testing. The method comprises the steps of constructing a small ubiquitin-like modifier fusion expression vector pSM, and recombinant expression vectors pSM1/VP0, pSM2/VP3 and pSM1/VP1, constructing a double expression recombinant vector pSM1/VP0-VP1, expressing and purifying protein, and conducting in-vitro assembly of the O type foot-and-mouth disease virus-like particles. The test paper comprises a base board. A detection layer is arranged on the top of the base board and comprises a detection line and a control line. An absorption layer is arranged on the side, near the control line, of the top of the detection layer. An immune-gold localization pad is arranged on the side, near the detection line, of the top of the detection layer, and a sample pad is arranged on the top of the immune-gold localization pad. The immune-gold localization pad is coated with colloidal gold particles. SPA markers are combined with the colloidal gold particles. The detection line is coated/infiltrated with A type foot-and-mouth disease virus-like particles, and the control line is coated/infiltrated with rabbit IgG. In this way, the detection period can be shortened, and real-time quick detection can be achieved on site.

The invention relates to a fluorescent brightening agent detection apparatus based on a cellphone platform and a detection method thereof, which can be used for detecting whether daily necessities contain a fluorescent brightening agent or not. The apparatus is composed of a clamping ring, a housing, a lens, a UV LED light source, a master control circuit board, an upper clamp plate, a torsion spring, a shaft, a lower clamp plate, and a rubber pad, when a to-be-detected sample is irradiated by the UV LED light source, excitant fluorescence is collected by a cellphone camera, image acquisitionis carried out by using a camera function of a cellphone, an comparison analysis is carried out on APP having image comparison and analysis functions, and whether the sample contains the fluorescent brightening agent or not can be obtained. The detection method is simple and convenient for operation, and the apparatus has the advantages of simple operation, convenient carrying, high testing precision, high security, and attractive price and quality.

The invention discloses a fluorescent whitening agent detecting wireless lens clamp based on a mobile phone and a fluorescent whitening agent detecting method. The wireless lens clamp comprises a light source part A, a plug-in lens B, a clamp part C, a main control circuit board, a Bluetooth module connected with the mobile phone in a wireless mode and mobile phone APP matched with the mobile phone, wherein the light source part A consists of a light shielding cover, an LED lamp group, a rechargeable lithium battery, a light source starting button and a housing; and the LED lamp group consistsof three pairs of LED lamps with different excitation wavelengths, which are separately near-ultraviolet, green-light and white-light light sources for exciting sample fluorescence, exciting sample fluorescence and performing camera light-supplementing illumination, and performing camera light-supplementing illumination; and the main control circuit board is used for controlling LED light sourcestarting, brightness regulation, hardware and App connecting protocols and the like. According to the fluorescent whitening agent detecting wireless lens clamp disclosed by the invention, the wirelesslens clamp is connected with the mobile phone camera through the clamp, and App can be combined to detect a fluorescent whitening agent in articles of daily use. The fluorescent whitening agent detecting wireless lens clamp has a small size, convenient carrying, high detecting precision and high detecting speed.

The invention relates to the field of animal disease detection and particularly relates to a flow-through Aleutian mink disease virus antigen and antibody detection kit, a preparation method thereof and application thereof. The kit provided by the invention comprises an Aleutian mink disease virus monoclonal antibody diagnostic solution and test paper, and the test paper comprises a nitrocellulosemembrane with detection points and a quality control point. The Aleutian mink virus monoclonal antibody diagnostic solution contains an Aleutian mink disease virus monoclonal antibody A1 marked by acolloidal gold, the detection points comprises a T1 point and a T2 point, the T1 point is coated with an Aleutian mink disease virus monoclonal antibody B1, the T2 point is coated with a standard Aleutian mink virus, and quality control point is coated with a quality control antibody. The kit has the function of simultaneously detecting the Aleutian mink virus antigen and antibody and can detect whether a sample is infected with the Aleutian mink virus and whether the Aleutian virus antibody is produced.

The invention discloses a carrier signal testing device and a testing method, the carrier signal testing device comprises a signal noise adjustor, and a carrier transmitter, a programmable attenuator,a noise simulator, a carrier receiver and a message comparator orderly connected in series; the signal noise adjustor generates a signal noise message which comprises a data message, an attenuation signal and a noise signal; the carrier transmitter generates a carrier signal according to the data message and transmits the carrier signal; the programmable attenuator adjusts strength of the carriersignal according to the attenuation signal; the noise simulator adds an interference signal to the carrier signal according to the noise signal; the carrier receiver receives the carrier signal and demodulates the data message; and the message comparator generates a comparison result according to the data message and the demodulated data message, and feeds back the comparison result to the signalnoise adjustor. The carrier signal testing device has the advantages of simple structure, clear detection result, random and non-single detection content, closed-loop cycling detection process, gooddetection effect, high efficiency, very high practicability and wide applicability.

The invention relates to an intelligent detection system and method for the micro-pore shape of a fuel injection nozzle based on machine vision. The system comprises an electron microscope, an image processing system, a master control system and a rotating stage, wherein the electron microscope, the image processing system and the rotating stage are connected with the master control system separately, the electron microscope is further connected with the image processing system and used for obtaining a primary observation image and secondary observation image of a micro-pore of the fuel injection nozzle, the image processing system is used for processing the primary observation image and the secondary observation image and judging whether or not the micro-pore is qualified and a deformed region of the micro-pore, a result image is displayed in real time, and the master control system is used for controlling the movement of the electron microscope and the rotation of the rotating stage.The intelligent detection system and method for the micro-pore shape of the fuel injection nozzle based on the machine vision have the advantages that the full automation and intellectualization of the micro-pore of the fuel injection nozzle are achieved, and the problems in the prior art that the manual detection efficiency is low, the labor intensity is large, the quality of classification is uneven, and the misjudgment rate is high are solved.

The invention provides an intelligent working condition detection vehicle, which includes a working condition detection vehicle body and an intelligent system arranged on the working condition detection vehicle body. The intelligent system includes an intelligent sensing system, an intelligent computer system and an aided driving system; the intelligent sensing system acquires the position of theworking condition detection vehicle body and position information of surrounding obstacles during detection of the working condition detection vehicle body, and an intelligent computer processes the information obtained by the intelligent sensing system, so as to obtain the motion control track of the working condition detection vehicle body and send a control signal to the aided driving system; and the aided driving system controls the working condition detection vehicle body to move according to control information of the intelligent computer. According to the intelligent working condition detection vehicle, the structure is compact, design is novel and reasonable, the volume is small, the weight is low, the intelligent degree is high, the detection efficiency is high, the detection accuracy is high, detection results are intuitive and clear, adaptability is high, practicability is high, the use effect is good, and the application and promotion value is high.

The invention discloses colloidal gold test paper for detecting Asia I-type foot-and-mouth disease and a preparation method of the colloidal gold test paper, and relates to the technical field of serology detection. The colloidal gold test paper comprises a bottom plate, wherein the top of the bottom plate is provided with a detection layer, the detection layer comprises a detection line and a reference line, an absorption layer is arranged at one side, adjacent to the reference line, of the top of the detection layer, an immunological gold-labeled pad is arranged at one side, adjacent to the detection line, of the top of the detection layer, and the top of the immunological gold-labeled pad is provided with a sample pad; and the immunological gold-labeled pad is coated with colloidal gold particles, the colloidal gold particles are combined with proteinG, the detection line is coated/infiltrated with purified proteins corresponding to Asia I-type foot-and-mouth disease virus sample particles, and the reference line is coated/infiltrated with rabbit IgG. A ratio of the colloidal gold of the immunological gold-labeled pad to proteinG is 2*104 to (1 to 2). The colloidal gold test paper can rapidly diagnose the Asia I-type foot-and-mouth disease in real time, and is relatively high in accuracy.