Colloidal gold test strip for detecting canine parvovirus

A canine parvovirus, colloidal gold test paper technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of incompatibility with early, rapid detection, difficulty in obtaining fresh red blood cells, and high cost of obtaining, and shorten the detection time and sensitivity. High and specific effect

Inactive Publication Date: 2014-04-23
HEBEI NORTH UNIV
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  • Abstract
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Problems solved by technology

Among them, the HA test is the most economical, but it also has disadvantages. On the one hand, it is difficult to obtain expensive and fresh red blood cells. On the other hand, this method is only suitable for the detection of samples in the late stage of CPV infection, and does not meet the requirements of early and rapid detection in recent years.
The ELISA method is widely used, but the operation requirements are relatively high, and it also needs a microplate reader to determine the results, so it is not suitable for grassroots use.

Method used

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  • Colloidal gold test strip for detecting canine parvovirus
  • Colloidal gold test strip for detecting canine parvovirus
  • Colloidal gold test strip for detecting canine parvovirus

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Embodiment

[0035] Canine parvovirus (Canine Parvovirus, CPV) can cause canine parvovirus disease, which is a highly contagious infectious disease of dogs. The clinical symptoms are mainly characterized by hemorrhagic enteritis or non-suppurative myocarditis. one of the most serious diseases in the industry. Among the three canine viral diseases, canine parvovirus, canine distemper, and canine coronavirus disease, canine parvovirus is the most serious, with an infection rate of 100% and a mortality rate of 10% to 50%. CPV belongs to the family Parvoviridae, the genus Parvovirus, a linear negative-sense single-stranded DNA virus with a genome length of 5323bp.

[0036] 1. Preparation and purification of polyclonal antibodies

[0037] Virus culture: Cultivate F81 cells in a 50mL cell flask. After 4 to 5 generations of culture, select cells that grow well and cover 80% of the bottom of the flask, pour off the culture supernatant, and add 9.5mL of 2% fetal bovine serum Add 500 μL of CPV to ...

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Abstract

The invention relates to the field of biological detection and in particular provides a colloidal gold test strip for detecting canine parvovirus. The colloidal gold test strip comprises a canine parvovirus antigen-coated polyclonal antibody and two anti-IgG nitrocellulose membranes of two strips, and a glass fiber membrane containing colloidal gold-labeled canine parvovirus antigen. According to the colloidal gold test strip, the canine parvovirus antigen is detected by adopting a double-antibody sandwich method and immunchromatography assay, the colloidal gold test strip has the advantages that the specificity is high, the operation is simple, the detection is rapid and accurate, complex equipment is not needed, and the requirements on clinical examination can be met.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a colloidal gold test strip for detecting canine parvovirus and an application thereof. Background technique [0002] Colloidal gold immunochromatography (Gold Immunochromatographic assay, GICA) is a marker diagnostic technique based on antigen and antibody specific reactions. In 1971, Faulk introduced colloidal gold into immunochemistry, thus giving birth to immune colloidal gold technology. In the 1980s, the immunocolloidal gold detection technology using NC membrane as a solid phase carrier emerged. This technology is a fast and simple new diagnostic technology developed on the basis of McAb, ELISA and other technologies. In 1995, Liu Yukai and others used immunochromatography as a rapid diagnostic test for early pregnancy, and then there was widespread application of human early pregnancy test strips. Hao Guilan and others used the immune colloidal gold test to complete t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983G01N33/577
Inventor 王净史利军李刚王鹏
Owner HEBEI NORTH UNIV
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