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Colloidal gold test paper for detecting Asia I-type foot-and-mouth disease and preparation method of colloidal gold test paper

A colloidal gold test strip, foot-and-mouth disease technology, applied in the field of serological detection, can solve the problems of FMD diagnosis and control difficulties, and achieve the effects of avoiding large-scale outbreaks, rapid detection, and high accuracy

Inactive Publication Date: 2017-05-10
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

In addition, FMDV also includes many subtypes and mutant strains. More than 80 subtypes have been found so far. The antigenicity of different subtypes or isolates within the same serotype is also different in varying degrees, and the degree of serological cross-reactivity is also different. Similarly, this characteristic creates certain difficulties for the diagnosis and prevention of FMD

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  • Colloidal gold test paper for detecting Asia I-type foot-and-mouth disease and preparation method of colloidal gold test paper

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Embodiment Construction

[0025] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0026] see figure 1 As shown, the embodiment of the present invention provides a colloidal gold test paper for detecting Asia I type foot-and-mouth disease, comprising a base plate 1 made of PVC, the top of the base plate 1 is provided with a detection layer 4, and the detection line 6 is provided with a detection line 6 and the control line 7, the top of the detection layer 4 is provided with an absorbing layer 5 near the side of the control line 7, and the side near the detection line 6 is provided with an immune gold standard pad 3, and the top of the immune gold standard pad 3 is provided with a sample pad 2 .

[0027] The immune gold standard pad 3 includes colloidal gold particles, which are combined with proteinG markers, the detection line 6 is coated / infiltrated with Asia I type foot-and-mouth disease virus-like particles, and the co...

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Abstract

The invention discloses colloidal gold test paper for detecting Asia I-type foot-and-mouth disease and a preparation method of the colloidal gold test paper, and relates to the technical field of serology detection. The colloidal gold test paper comprises a bottom plate, wherein the top of the bottom plate is provided with a detection layer, the detection layer comprises a detection line and a reference line, an absorption layer is arranged at one side, adjacent to the reference line, of the top of the detection layer, an immunological gold-labeled pad is arranged at one side, adjacent to the detection line, of the top of the detection layer, and the top of the immunological gold-labeled pad is provided with a sample pad; and the immunological gold-labeled pad is coated with colloidal gold particles, the colloidal gold particles are combined with proteinG, the detection line is coated / infiltrated with purified proteins corresponding to Asia I-type foot-and-mouth disease virus sample particles, and the reference line is coated / infiltrated with rabbit IgG. A ratio of the colloidal gold of the immunological gold-labeled pad to proteinG is 2*104 to (1 to 2). The colloidal gold test paper can rapidly diagnose the Asia I-type foot-and-mouth disease in real time, and is relatively high in accuracy.

Description

technical field [0001] The invention relates to the technical field of serological detection, in particular to a colloidal gold test paper for detecting Asia I type foot-and-mouth disease and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious infectious disease caused by FMD virus (FMDV). The virus is mainly divided into seven serotypes: A, O, C, SAT1, SAT2, SAT3 and Asia I, and cross-immune protection cannot be produced between each serotype. In addition, FMDV also includes many subtypes and mutant strains. More than 80 subtypes have been found so far. The antigenicity of different subtypes or isolates within the same serotype is also different in varying degrees, and the degree of serological cross-reactivity is also different. Again, this characteristic poses certain difficulties to the diagnosis and prevention of FMD. Contents of the invention [0003] In view of the defects in the prior ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 孙世琪郭慧琛张韵常艳燕魏衍全茹嘉喜智晓莹杜平刘湘涛殷宏罗建勋
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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