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Cardiac troponin I detection reagent, preparation method and cardiac troponin I detection kit

A technique for cardiac troponin and detection kits is applied in the preparation of the detection reagents, cardiac troponin I detection reagents, and cardiac troponin I detection kits, and can solve the problem of reduced sensitivity, material loss, troponin I antibody fully reacts and other problems to achieve the effect of increasing the reaction area, improving the reaction speed and uniform distribution

Active Publication Date: 2020-10-02
SOPHONIX CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, in the process of isolating the combination of cardiac troponin I and antibody from the reaction system, many steps will cause material loss, for example, troponin I may not be able to fully react with the antibody during the reaction , the stationary phase may not be completely separated during the separation process, etc.
When the concentration of cardiac troponin I in the sample is low, the loss during the reaction and separation process may lead to the undetectable cardiac troponin I in the final detection, resulting in a decrease in the sensitivity of the detection

Method used

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  • Cardiac troponin I detection reagent, preparation method and cardiac troponin I detection kit
  • Cardiac troponin I detection reagent, preparation method and cardiac troponin I detection kit
  • Cardiac troponin I detection reagent, preparation method and cardiac troponin I detection kit

Examples

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preparation example 1

[0076] Preparation Example 1: The preparation of the buffer solution specifically includes the following configuration steps.

[0077] 1. Preparation of buffer solution 1: Weigh 14.8g of triethanolamine and 5.8g of sodium chloride, dissolve them in 100mL of purified water, adjust the pH value to 7.3 with 4mM hydrochloric acid and 4mM sodium hydroxide, and dilute to 1000ml with purified water, Filter with a 0.22 μm filter membrane to obtain buffer solution 1.

[0078] 2. Preparation of buffer 2: Weigh 75.0 g of glycine, dissolve it in 100 mL of purified water, dilute to 1000 ml with purified water, and then filter through a 0.22 μm filter membrane to obtain buffer 2.

[0079] 3. Preparation of buffer 4: Weigh 10.0g of sodium tetraborate decahydrate, dissolve it in 100mL of purified water, adjust the pH value to 11.0 with 4mM hydrochloric acid and 4mM sodium hydroxide, and dilute to 1000ml with purified water, then use 0.22μm filter membrane to obtain buffer 3.

[0080] 4. Pre...

preparation example 2

[0085] Preparation example 2: the preparation of the buffer solution, specifically including the following configuration steps.

[0086] 1. Preparation of buffer solution 1: Weigh 15.1g of triethanolamine and 6.0g of sodium chloride, dissolve them in 100mL of purified water, adjust the pH value to 7.6 with 4mM hydrochloric acid and 4mM sodium hydroxide, and dilute to 1000ml with purified water, Filter with a 0.22 μm filter membrane to obtain buffer solution 1.

[0087] 2. Preparation of buffer 2: Weigh 75.0 g of glycine, dissolve it in 100 mL of purified water, dilute to 1000 ml with purified water, and then filter through a 0.22 μm filter membrane to obtain buffer 2.

[0088] 3. Preparation of buffer 4: Weigh 7.0g of sodium tetraborate decahydrate, dissolve it in 100mL of purified water, adjust the pH value to 9.0 with 4mM hydrochloric acid and 4mM sodium hydroxide, and dilute to 1000ml with purified water, then use 0.22μm filter membrane to obtain buffer 4.

[0089] 4. Pre...

preparation example 3

[0094] Preparation Example 3: The configuration method of the buffer solution. The difference from Preparation Example 1 is that the configuration method of the buffer solution 5 is as follows: Weigh 300 g of dipotassium hydrogen phosphate and 120 g of potassium dihydrogen phosphate and dissolve them in 100 mL of purified water, pass through 4 mM hydrochloric acid and 4 mM Sodium hydroxide was used to adjust the pH value to 6.4, and the volume was adjusted to 1000 ml with purified water, and then filtered with a 0.22 μm filter membrane to obtain buffer solution 5.

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Abstract

The invention relates to a cardiac troponin I detection reagent, a preparation method and a cardiac troponin I detection kit. The cardiac troponin I detection reagent comprises a coated antibody solution, a detection antibody solution and an indicator solution, wherein the coated antibody comprises an antibody and stationary phase conjugate 1 obtained by coupling an antibody 1 with a stationary phase, and an antibody and stationary phase conjugate 2 obtained by coupling an antibody 2 with a stationary phase; the detection antibody is obtained by coupling an antibody 3 with a marker; and the indicator solution contains an indicator for quantitatively detecting the concentration of the marker. The invention also comprises the preparation method of the detection reagent and a kit using the detection reagent. In the invention, the antibody 2 and the antibody 3 can be respectively combined with the cardiac troponin I, so that the sufficient separation of the cardiac troponin I is facilitated, and the detection sensitivity of the reagent to the cardiac troponin I is further improved.

Description

technical field [0001] The invention relates to the technical field of health detection, in particular to a cardiac troponin I detection reagent, and also relates to a preparation method of the detection reagent. In addition, the present invention also relates to a cardiac troponin I detection kit. Background technique [0002] Cardiovascular disease has become the number one killer of human health in the 21st century. Cardiac troponin is currently the most sensitive and specific marker of myocardial injury in the diagnosis of myocardial necrosis, and is the main basis for the diagnosis of acute myocardial infarction (AMI) and risk stratification of acute coronary syndrome (ACS). Cardiac troponin is a complex composed of three subunits, namely cardiac troponin C, cardiac troponin I and cardiac troponin T. Cardiac troponin I is considered to be one of the most specific and sensitive serum markers of myocardial injury. Because of its high myocardial specificity, high sensiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/543G01N33/535
CPCG01N33/6887G01N33/6893G01N33/581G01N33/54326G01N33/535G01N2333/4712G01N2800/324
Inventor 张明琛孙佳李博飞
Owner SOPHONIX CO LTD
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