Magnetic graphene oxide composite material immobilized horse radish peroxidase as well as preparation method and application thereof
A technology of horseradish peroxidase and composite materials, which is applied in biochemical equipment and methods, oxidoreductases, chemical instruments and methods, etc., can solve the problems of short catalytic life and enzyme inactivation of horseradish peroxidase. , to achieve the effect of improving the ability of reuse, improving activity and facilitating recovery
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[0058] Example 1: Preparation of a novel magnetic graphene oxide composite material
[0059] (1) Synthesis of carboxylated graphene oxide GO-COOH
[0060] Graphene oxide (GO) is synthesized by Hummers method. First, take 0.3 g graphite powder, 0.7 g sodium nitrate in 23 mL 12mol L -1 The concentrated sulfuric acid solution was stirred in an ice bath for 15 minutes. Slowly add 2.0 g of potassium permanganate and transfer to a 40°C water bath and stir for 90 minutes. Add 25 mL of deionized water to the reaction system for dilution and continue stirring for 10 minutes. Add 4 mL of hydrogen peroxide, the system becomes a golden yellow solution, let stand for 24 hours, pour out the supernatant, filter with suction to obtain the precipitate, and wash the precipitate with 5% hydrochloric acid repeatedly until BaCl 2 The supernatant was tested for no precipitation, and then washed with deionized water and centrifuged until the pH of the supernatant was 7, and the washing was stopped. P...
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[0065] Example 2: Preparation of a novel magnetic graphene oxide composite material
[0066] (1) Synthesis of carboxylated graphene oxide GO-COOH
[0067] Graphene oxide (GO) is synthesized by Hummers method. First, take 0.7 g graphite powder, 0.3 g sodium nitrate in 23 ml 12mol L -1 The concentrated sulfuric acid solution was stirred in an ice bath for 15 minutes. Slowly add 6.0 g of potassium permanganate and transfer to a 40°C water bath and stir for 90 minutes. Add 75 ml of deionized water to the reaction system for dilution and continue stirring for 10 minutes. Add 8 ml of hydrogen peroxide, the system becomes a golden yellow solution, let stand for 24 hours, pour out the supernatant, filter with suction to obtain the precipitate, and wash the precipitate with 5% hydrochloric acid repeatedly until BaCl 2 The supernatant was tested for no precipitation, and then washed with deionized water and centrifuged until the pH of the supernatant was 7, and the washing was stopped. P...
Example Embodiment
[0072] Example 3: Preparation of a novel magnetic graphene oxide composite material
[0073] (1) Synthesis of carboxylated graphene oxide GO-COOH
[0074] Graphene oxide (GO) is synthesized by Hummers method. First, take 0.5 g graphite powder, 0.5 g sodium nitrate in 23 ml 12mol L -1 The concentrated sulfuric acid solution was stirred in an ice bath for 15 minutes. Slowly add 4.0 g of potassium permanganate and transfer to a 40°C water bath and stir for 90 minutes. Add 50 ml of deionized water to the reaction system to dilute and continue to stir for 10 minutes. Add 6 ml of hydrogen peroxide, the system becomes a golden yellow solution, let stand for 24 hours, pour out the supernatant, suction filtration to obtain the precipitate, the precipitate is repeatedly washed with 5% hydrochloric acid until BaCl 2 The supernatant was tested for no precipitation, and then washed with deionized water and centrifuged until the pH of the supernatant was 7, and the washing was stopped. Pour ...
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