Microcarrier for culturing animal cell and crosslinking method thereof

Abstract

The invention discloses a microcarrier for culturing animal cell and a crosslinking method thereof. The microcarrier is a microcarrier of ADCF which is synthesized by the following steps of taking Arginine or fully-synthesized Poly-Arginine as aglucon, and crosslinking the aglucon with the surface of dextran bead by taking allyl glycidyl ether as crosslinking activating agent. The microcarrier is polypeptide chain which is formed by polymerizing the arginine, has the property which is similar to animal gelatin, provides an adhering growing environment with ploy positive charge for the cell, and is good for the fast adhering and spreading of the cell and the high-intensity growing of the cell. The Arginine aglucon not only imitates the surface structure of the animal gelatin, but also does not induce animal-derived substances, and overcomes the problems of the quality and the safety of the product caused by inducing the animal-derived substances during large-scale cell culture.

Description

technical field [0001] The invention relates to a microcarrier for animal cell culture, more specifically to a microcarrier containing arginine ligands without animal-derived components and a method for crosslinking arginine ligands and substrates, belonging to biochemical engineering and cell engineering field, Background technique [0002] Microcarrier culture is currently recognized as the most promising large-scale culture technology for animal cells. It has been widely used to produce enzymes, growth factors, vaccines and monoclonal antibodies with important medical value, as well as effector cells with special functions. It has become a key technology in the medical and biological high-tech industry. [0003] Animal cell culture often requires adherent growth, and cells in suspension grow slowly, stop, or even die. The principle of microcarrier culture is to add the harmless particles-microcarriers to the culture medium of the culture container as a carrier to make t...

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the risk of virus introduction caused by the application of microcarriers containing animal-derived components in the process of large-scale cell culture and the resulting difficulties in product declaration and supervision, there is an urgent need in the market for an animal-derived microcarrier that does not contain animal collagen. Materials as ligands for novel microcarriers to establish animal-derived component-free cell culture systems and protocols throughout the production process

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