Five-classification leucocyte simulacrum particle, method for preparing same, and quality control substance and calibration substance containing same

A technology for simulating particles and white blood cells, applied in the field of blood cell analysis, which can solve the problems of complex red blood cell removal process, reduced process practicability and controllability, and difficulty in using enhanced aldehyde fixation technology.

Active Publication Date: 2009-10-21
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) It is difficult to obtain white blood cell particles, which directly leads to increased cost and complicated process
[0006] In the blood of humans and animals, the concentration of white blood cells is only 1/100-1/1000 of that of red blood cells, and the process of obtaining white blood cells without red blood cells is very complicated
However, the use of hemolytic agents may lead to changes in the characteristics of leukocytes, so that the physiological leukocyte state can no longer be used to evaluate the purified leukocyte particles
In addition, the process of removing red blood cells is very complicated, and it is not easy to achieve satisfactory results even if high-cost cell separation fluid is used, so the cost and products of the calibrator and quality control materials of the five-differential blood cell analyzer are greatly lim

Method used

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  • Five-classification leucocyte simulacrum particle, method for preparing same, and quality control substance and calibration substance containing same
  • Five-classification leucocyte simulacrum particle, method for preparing same, and quality control substance and calibration substance containing same
  • Five-classification leucocyte simulacrum particle, method for preparing same, and quality control substance and calibration substance containing same

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0102] Example 1: Using human red blood cells to simulate lymphocytes

[0103] 1. Configure the following treatment reagents: add 1.5g sodium chloride, 1g sodium nitrite, 100μl of 1% (w / v) sodium cetyl sulfonate and 100μl of 25% (v / v) pentane in 195ml pure water Dialdehyde

[0104] 2. Mix 5ml of human anticoagulated whole blood with the above treatment reagents, and place them at room temperature for about 30 minutes;

[0105] 3. Test on the machine (BC5500), and determine whether to start strengthening fixation according to different distribution requirements;

[0106] 4. When the reactant meets the required distribution requirements (that is, when the DIFF image is already located in the lymphocyte area), add a 1:10 glutaraldehyde-formaldehyde mixture with a final concentration of 0.5% (v / v) to the reaction solution , Place at room temperature for 2 hours.

[0107] 5. Test on the computer to observe the changes in counting and cell distribution;

[0108] 6. After meeting the req...

Example Embodiment

[0109] Example 2: Using elephant red blood cells to simulate neutrophils

[0110] 1. Configure the following treatment reagents: add 1.5g sodium chloride, 1g sodium nitrite, 100μl of 1% (w / v) sodium cetyl sulfonate and 100μl of 25% (v / v) pentane in 195ml pure water Dialdehyde

[0111] 2. Mix 5ml of elephant anticoagulant whole blood with the treatment reagent, and leave it at room temperature for about 30 minutes;

[0112] 3. Test on the computer, and determine whether to start strengthening the fixation according to different distribution requirements;

[0113] 4. When the reactant meets the required distribution requirements (that is, when the DIFF image is already in the neutrophil area), add glutaraldehyde-formaldehyde with a final concentration of 0.5% (v / v) 1:10 to the reaction solution The mixed solution was left at room temperature for 2 hours.

[0114] 5. Measure on the computer (BC5500), observe the count and cell distribution changes;

[0115] 6. After meeting the requi...

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Abstract

The invention relates to a method for preparing five-classification leucocyte simulacrum particle by erythrocyte, comprising the following steps: selecting proper erythrocyte, processing the erythrocyte by multifunctional reagent to achieve the integrity of erythrocyte membrane and synchronous regulation of volume, shape and inclusion, intensifying and fixing the erythrocyte, and preserving afterwashing. The invention also relates to a leucocyte simulacrum particle prepared by the method, reagent system used for preparing the leucocyte simulacrum particle, and quality control substance and calibration substance used for hematology analyzer and containing the simulacrum particle.

Description

technical field [0001] The invention relates to the field of blood cell analysis, more specifically, to a five-class leukocyte simulant particle prepared by using red blood cells, a preparation method thereof, and a quality control substance and a calibrator containing the simulant particle. Background technique [0002] In the five-differential hematology analyzer using optical detection technology, the white blood cell particle simulant in its quality control and calibrator is very different from the white blood cell simulant used in the simple impedance method (see US 4,704,364) (see figure 1 ), not only must have a relatively good volume distribution, but also need to have a good cell optical complexity distribution, and some particles also need to have specific fluorescence characteristics. Since the red blood cells of humans or animals are biconcave or spindle-shaped, they cannot show the optical characteristics of concentrated distribution in the optical detection of ...

Claims

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Application Information

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IPC IPC(8): G01N33/96G01N33/48C12N5/078
CPCC12N5/0641C12N2500/60C12N2503/00
Inventor 张晖王璐徐祖越刘牧龙张丽
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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