Quadruple PCR detection method capable of identifying canine parvovirus, hundestaupe virus, canine parainfluenza virus and canine adenovirus type-2

A technology of canine parainfluenza virus and canine distemper virus, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganism measurement/testing, can solve mixed infection, time-consuming, difficult clinical differential diagnosis and other problems, Achieve the effect of good specificity, simple operation and fast detection speed
CN106521036AActive Publication Date: 2017-03-22INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
Publication Date
2017-03-22

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Abstract

The invention discloses a quadruple PCR detection method capable of identifying canine parvovirus, hundestaupe virus, canine parainfluenza virus and canine adenovirus type-2, belonging to the field of virus detection. With the adoption of the method, four viruses including canine parvovirus, hundestaupe virus, canine parainfluenza virus and canine adenovirus type-2 can be simultaneously detected, the operation is simple, the detection speed is high, and the throughput is high; the accuracy is high, the specificity is good, the repeatability is good, the analysis can be accurately and rapidly carried out at high throughput, and the method is suitable for being popularized and applied in clinical practice. A primer provided by the invention is good in specificity, can be combined with the four target viral nucleic acids needing to be detected, and can not be combined with other common viral nucleic acids, such as feline distemper, canine adenovirus type I and leptospira canicola.
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Description

technical field

[0001] The invention belongs to the field of virus detection, in particular to a quadruple PCR detection method for distinguishing canine parvovirus, canine distemper virus, canine parainfluenza virus and canine adenovirus type 2. Background technique

[0002] Canine distemper, canine parvovirus, canine parainfluenza, and canine adenovirus type 2 are common infectious diseases in dogs with high morbidity and mortality, causing serious losses to the dog industry. Canine parvovirus disease is an acute infectious disease of dogs caused by canine parvovirus (Canine Parvovirus, CPV), characterized by acute hemorrhagic gastroenteritis or non-suppurative myocarditis, and dogs of all ages and genders are prone to Sensitive, but the susceptibility of puppies is higher, the case fatality rate reaches 50%. Canine distemper is caused by canine distemper virus (Canine Distemper Virus, CDV), a highly contagious disease of many animals in dogs and carnivores, characterized...

Claims

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