Kit for detecting canine parainfluenza virus

Abstract

The invention discloses a kit for detecting a canine parainfluenza virus. The kit comprises a light shielding detection box, ALL-READY PCR BEADS full premix freeze-dried powder, a positive control group, a negative control group and a photodetector, the light shielding detection box and the photodetector are arranged in a fitting manner, a photoelectric detection module is arranged in the photoelectric detector, and the ALL-READY PCR BEADS full premix freeze-dried powder comprises transcriptase, a nucleic acid amplifier enzyme, a reaction buffer solution, a specific primer and a probe which are needed by the fluorescent quantitative PCR detection of the canine parainfluenza virus RNA; and the negative control group adopts canine parainfluenza virus RNA-free enzyme water, the positive control group is in vitro transcribed parainfluenza virus RNA, and the photoelectric detection module includes a photodiode. The design is optimized, the light shielding detection box is combined, and the photoelectric detection module is optimized, so the detection error is reduced, and the detection specificity is improved.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a kit for detecting canine parainfluenza virus. Background technique [0002] Canine parainfluenza is an infectious disease in dogs caused by canine parainfluenza virus (CPIV, hereinafter referred to as CPIV). The disease is widespread. The virus invades the dog's respiratory system, causing inflammation of the respiratory system. The clinical manifestations are fever, coughing, and runny nose. It is often mixed with bacteria, mycoplasma and other viruses, which makes the dog's condition worse. Canine parainfluenza is currently one of the main infectious diseases in the dog industry, especially in pet dogs. [0003] CPIV is a member of the genus Mumps virus and the family Paramyxoviridae. The viral genome is single-stranded, not segmented, negative-strand RNA virus, about 15kb in length, including encoding 8 structural proteins (NP, P, V, M, SH, HN, F, L) seven segments...

AI Technical Summary

Problems solved by technology

The detection methods for antibodies include immunofluorescence, neutralization experiment and hemagglutination inhibition test, etc. The ATP fluorescence detection instrument converts weak fluorescence into electrical signals through photoelectric sensors, and completes data reading and analysis through analog-to-digital conversion combined with a single-chip microcomputer. In order to obtain the detection result, however, in the detection process of the existing ATP fluorescence detection instrument, in order to strengthen the signal transmission, an amplification circuit is usually set up, but the amplification circuit will generate a bias current, causing an error voltage, thereby affecting the detection signal of the photoelectric sensor , which reduces the detection accuracy, and its overall shading effect is poor, and it is easy to affect the detection data

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