Multiplex pcr detection primer set for canine distemper virus, canine adenovirus type Ⅱ and canine parainfluenza virus

Abstract

The invention provides a multi-PCR (Polymerase Chain Reaction) detecting primer group for a canine distemper virus, a canine adenovirus type 2 and a canine parainfluenza virus. The multi-PCR detecting primer group comprises a primer pair A, a primer pair B and a primer pair C, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; the primer pair C comprises a primer 5 and a primer 6; the nucleotide sequences of the primer 1, the primer 2, the primer 3, the primer 4, the primer 5 and the primer 6 are respectively a sequence 1, a sequence 2, a sequence 3, a sequence 4, a sequence 5 and a sequence 6 in sequence tables. The multi-PCR detecting primer group has the advantages of simplicity in operation, high sensitivity, strong specificity, good repeatability, low cost and time consumption and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a multiplex PCR detection primer set for canine distemper virus, canine adenovirus type II and canine parainfluenza virus. Background technique [0002] Canine distemper virus belongs to Paramyxoviridae in classification and is a single-stranded RNA virus. At room temperature, the virus is relatively unstable, especially sensitive to ultraviolet rays, dryness, and high temperatures above 50-60°C. Under freezing conditions, the virus can survive for several weeks. [0003] Canine adenovirus type Ⅱ can cause infectious laryngotracheitis and pneumonia symptoms in dogs. Clinical features include persistent high fever, cough, serous to mucous rhinorrhea, tonsillitis, laryngotracheitis, and pneumonia. According to the statistics of clinical incidence, the disease is more common in puppies under 4 months old, and can cause coughing in the whole litter or whole group in puppies, so the dis...

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Problems solved by technology

The colloidal gold test strip method is that a test strip can only detect a single canine disease, and cannot detect three diseases at the same time, and the cost is very high. It usually costs 50-100 yuan to detect one disease

The cost of diagnostic reagents in the fluorescent probe technology method is relatively high, and expensive gene chip spotters and gene chip scanners are required for detection, which is not suitable for the promotion and application of grassroots units, and the detection time is long, and it takes at least 5-7 days to detect samples. Hours

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