Test strip for detecting feline coronavirus, preparation method thereof, kit and detection method

A feline coronavirus and test strip technology, which is applied in the field of feline coronavirus test strips and its preparation, to achieve the effects of simple steps, improved reagent sensitivity, and simple operation

Pending Publication Date: 2021-02-26
爱若维生物科技(苏州)有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is mainly aimed at the problem of how to detect felin...

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  • Test strip for detecting feline coronavirus, preparation method thereof, kit and detection method
  • Test strip for detecting feline coronavirus, preparation method thereof, kit and detection method
  • Test strip for detecting feline coronavirus, preparation method thereof, kit and detection method

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Embodiment Construction

[0025] In order to make the purpose, technical solution and advantages of the present application clearer, the technical solution of the present application will be clearly and completely described below in conjunction with specific embodiments of the present application and corresponding drawings. Apparently, the described embodiments are only some, not all, embodiments of the present application, and are not intended to limit the scope of the present invention. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of this application.

[0026] In order to detect feline coronavirus quickly and accurately, the application provides a test strip for detecting feline coronavirus, such as figure 1As shown, the test strip includes a bottom plate 1, on which the sample cushion layer 2, colloidal gold cushion layer 3, nitrocellulose film layer 4 and water-a...

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Abstract

The invention relates to a test strip for detecting feline coronavirus and a kit prepared from the test strip, the test strip comprises a bottom plate, and a sample pad layer, a colloidal gold pad layer, a nitrocellulose membrane layer and a water absorption layer are sequentially lapped on the bottom plate from left to right; the colloidal gold pad layer is coated with a colloidal gold particle labeled feline coronavirus monoclonal antibody and a feline infectious peritonitis virus polyclonal antibody; the nitrocellulose membrane layer is coated with a feline coronavirus monoclonal antibody as a T1 detection line, a feline infectious peritonitis virus polyclonal antibody as a T2 detection line and goat anti-chicken IgG as a quality control line. The feline coronavirus antigen is detectedin the form of the test strip and the kit, the operation is simple, real POCT detection is achieved, and the virus transmission is effectively controlled. In addition, due to the polyantibody labelingand polyantibody coating form in the test strip, the reagent sensitivity is improved to the maximum extent, and the detection rate is increased.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a test strip for detecting feline coronavirus, a preparation method, a kit and a detection method thereof. Background technique [0002] Feline infectious peritonitis (FIP) is caused by infection with feline coronavirus. The disease was first discovered in the 1960s. FIP is divided into exudative and non-exudative types. As the disease progresses, non-exudative FIP can be converted It is exudative FIP. The main clinical features of exudative FIP are fibrinous peritonitis, pleurisy, and pericarditis accompanied by abdominal, pleural, or pericardial effusions; non-exudative FIP has no obvious effusion, and the main clinical features are granuloma-like lesions in different organs, including the eyes. and central nervous system. Both exudative FIP and nonexudative FIP are associated with severe systemic disease and produce varying degrees of pleural or peritoneal effus...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/543G01N33/52
CPCG01N33/577G01N33/56983G01N33/558G01N33/54346G01N33/52G01N2333/165G01N2469/10
Inventor 王蓉蓉冯小龙孙云雷
Owner 爱若维生物科技(苏州)有限公司
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