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Specific culture medium for Lactobacillus plantarum and its application

A technology of Lactobacillus plantarum and culture medium, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms. It can solve the problems of expensive and unspecified detection and counting methods of Lactobacillus plantarum, and achieve low cost and convenient configuration. Effect

Active Publication Date: 2018-11-30
JUNLEBAO DAIRY GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The detection and counting methods of Lactobacillus plantarum are not stipulated in the national standard of our country. At present, the identification or quantitative detection methods of Lactobacillus plantarum are mainly PCR or fluorescent quantitative PCR method of molecular biology, such as the application number 201210594264. Quantitative detection method of Lactobacillus and its detection component box and application” Chinese invention patent application discloses a method for quantitative detection of Lactobacillus plantarum, which is a PCR detection technology, specifically extracting the total RNA of the sample to be tested and reverse transcription For cDNA, specific primers are used for fluorescent quantitative PCR, and the number of Lactobacillus plantarum is determined by comparing the Ct value of the sample to be tested with the standard curve of the quantitative external standard. Although this method produces results quickly, the disadvantage is that this method requires precise Instruments, expensive components and demanding operators

Method used

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  • Specific culture medium for Lactobacillus plantarum and its application
  • Specific culture medium for Lactobacillus plantarum and its application
  • Specific culture medium for Lactobacillus plantarum and its application

Examples

Experimental program
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Effect test

Embodiment 1-6

[0034] Embodiment 1-6 A kind of specific culture medium of Lactobacillus plantarum

[0035] Examples 1-6 are a specific culture medium for Lactobacillus plantarum, the components and contents of which are shown in Table 1, and the preparation conditions are shown in Table 2.

[0036] Table 1 The components and contents contained in the specific culture medium of Lactobacillus plantarum

[0037]

[0038] Table 2 Preparation conditions of specific medium for Lactobacillus plantarum

[0039]

[0040] During the preparation of the medium for the Lactobacillus plantarum-specific medium provided in the above-mentioned Examples 1-6, other components except lincomycin were added together. When the medium was used, the medium was first melted, When the temperature drops to 37-50°C, add lincomycin.

[0041] The above-mentioned Lactobacillus plantarum-specific culture medium is easy to configure and low in cost. The culture medium contains substances such as lincomycin and carbon...

Embodiment 7

[0042] Example 7 A method for counting live bacteria of Lactobacillus plantarum

[0043] Embodiment 7 is to provide the application of the specific culture medium of Lactobacillus plantarum provided in Examples 1-6, that is, different strains (including Lactobacillus plantarum) are respectively provided in Examples 1-6 for the specificity of Lactobacillus plantarum. Cultured on the cultured medium, and the cultured strains were counted on the plate.

[0044] The test samples are yogurt and lactic acid bacteria drinks containing Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus casei, Lactobacillus plantarum, Bifidobacterium lactis, Lactobacillus acidophilus, Lactobacillus rhamnosus, and the specific experimental steps are as follows:

[0045] (1) Sample dilution

[0046] Liquid sample: shake the liquid sample well, draw 25ml of the sample with a sterile pipette, mix it in 225ml of normal saline, and make a 1:10 sample dilution;

[0047] (2) Take 1ml of the ...

Embodiment 8-10

[0050] Embodiment 8-10 carries out the method for living bacteria count to Lactobacillus plantarum

[0051] The method for counting live bacteria of Lactobacillus plantarum provided by Examples 8-10 is the same as Example 7, except that the temperature and time of cultivation in the incubator in step (3) are different from Example 7, specifically Yes:

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Abstract

The invention discloses a specific culture medium for Lactobacillus plantarum, which is convenient to configure and low in cost. The culture medium contains substances such as lincomycin and carbon source. Plantarum can be cultured and counted specifically in fermented milk, lactic acid bacteria drinks or bacteria powder; the present invention also provides an application utilizing the above-mentioned plantarum specific culture medium, which is used for plantarum lactobacillus To count viable bacteria is to culture the strains on the culture medium, and then count the cultured bacterial strains on plates. This counting method is simple and has high specificity. Compared with the PCR counting method, it is more difficult for operators. Lower requirements and lower costs. The invention is suitable for configuring a culture medium capable of specifically counting the number of viable Lactobacillus plantarum.

Description

technical field [0001] The invention belongs to the field of microbial detection, and relates to a culture medium and a method for counting viable bacteria on bacterial strains, in particular to a specific culture medium for Lactobacillus plantarum and its application. Background technique [0002] Lactobacillus plantarum is a type of Lactobacillus, often found in fermented vegetables and fruit juices, most of which are isolated from plants, so it is named Lactobacillus plantarum. According to my country's "List of Strains Available for Food", Lactobacillus plantarum is one of the probiotic strains that can be used for food. It can well tolerate the gastric acid and bile of the human body and play a role in regulating the balance of intestinal flora. [0003] Studies have shown that Lactobacillus plantarum has beneficial health functions such as enhancing immunity, lowering cholesterol, preventing cardiovascular diseases, and regulating intestinal flora. Therefore, Lactobac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 冯丽莉王华王世杰朱宏薛玉玲张栋马蕊丛懿洁
Owner JUNLEBAO DAIRY GRP CO LTD
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