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Production method capable of improving output of lincomycin through adding glutamine

A technology of glutamine and lincomycin is applied in the biological field to achieve the effect of increasing yield

Inactive Publication Date: 2011-11-23
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on whether the glutamine added in the fermentation process can increase the yield of lincomycin

Method used

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  • Production method capable of improving output of lincomycin through adding glutamine
  • Production method capable of improving output of lincomycin through adding glutamine
  • Production method capable of improving output of lincomycin through adding glutamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～5

[0020] Effect of Adding Amount of Glutamine on Biosynthesis of Lincomycin

[0021] When preparing the lincomycin fermentation medium, add glutamine with a weight ratio of 0.005%, 0.02%, 0.04%, 0.06%, and 0.1% to the medium, and use the medium without glutamine as a control, That is, in Control Example 1, Streptomyces lincolnensis was inoculated, and after 168 hours of fermentation, samples were taken to determine the potency of lincomycin. The results are shown in Table 1.

[0022] Table 1. The effect of the amount of glutamine added on the biosynthesis of lincomycin

[0023]

[0024] It can be seen from the results in Table 1 that adding glutamine to the fermentation medium can significantly increase the yield of lincomycin, and the preferred addition amount is 0.04-0.06%.

Embodiment 6～9

[0026] Effect of Glutamine Addition Time on Lincomycin Biosynthesis

[0027] Prepare lincomycin fermentation medium, insert Streptomyces lincolnensis (Streptomyces lincolnensis), carry out fermentation culture, add the glutamine that is 0.04% by weight to fermentation medium respectively in 72, 96, 120, 144 hours, simultaneously with The culture medium without glutamine was used as a control. After 168 hours of fermentation, samples were taken to determine the titer of lincomycin. The results are shown in Table 2.

[0028] Table 2. The effect of the addition time of glutamine on the biosynthesis of lincomycin

[0029]

[0030] It can be seen from the results in Table 2 that adding glutamine to the medium at 0 hours of fermentation can significantly increase the yield of lincomycin, and the preferred adding time is 72-96 hours.

[0031] In summary, adding glutamine during the fermentation process of Streptomyces lincoeum can significantly increase the yield of lincomycin by...

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PUM

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Abstract

The invention relates to a production method capable of improving the output of lincomycin through adding glutamine. The production method is characterized in that: the glutamine is added in the fermentation process; the quantity of added glutamine is 0.04-0.06% of the weight of a culture medium; and the adding time of the glutamine is 72-96 hours. The production method has the following advantage that: the output of the lincomycin can be obviously improved through adding the glutamine which does not serve as a precursor for synthesizing the lincomycin in the fermentation process.

Description

【Technical field】 [0001] The invention relates to the technical field of biotechnology, in particular to a production method for increasing the yield of lincomycin by adding glutamine in the fermentation process. 【Background technique】 [0002] Lincomycin (Lincomycin, referred to as LCM), also known as lincomycin, belongs to the lincosamide antibiotics. It was isolated from the soil near Lincoln, Nebraska in 1962 and obtained from the bacteria. Lincosamides were isolated from the culture medium. Lincomycin has strong resistance to Gram-positive aerobic bacteria, has no cross-infection to existing antibiotics, and has low toxicity. Therefore, it is used clinically to treat diseases caused by Gram-positive bacteria important drugs. [0003] The main components of lincomycin are lincomycin A, lincomycin B, lincomycin C, lincomycin D and lincomycin S. Generally speaking, lincomycin refers to its A component, its chemical name is 6-[1-methyl-trans-4-propyl-L-2-pyrrolidinecarbo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/64C12R1/565
Inventor 叶蕊芳乔现婷张宏周毛全贵吕和平刘守强王美珠
Owner EAST CHINA UNIV OF SCI & TECH
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