Method for increasing erythromycin yield by transforming saccharopolyspora erythraea SACE_3980 gene

A technology of Saccharopolyspora erythromycin and erythromycin, which is applied in the field of genetic engineering, can solve the problems such as the lack of research on the regulation genes of the TetR family of secondary metabolism of Saccharopolyspora erythromycin, and the unclear elucidation of the precise mechanism of erythromycin.

Active Publication Date: 2017-03-22
ANHUI UNIVERSITY
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Problems solved by technology

There are 101 TetR family genes in the whole genome of Rhodomycetes Saccharopolyspora. However, there are still few studies on the regulatory genes of the TetR family in the secondary metabolism of Rhodomycetes Saccharopolyspora. SACE_7040, SACE_0012 and genes regulating erythromycin synthesis and metabolism SACE_5599, SACE_3986, SACE_3446, SACE_7301, etc., and the precise mechanism for regulating erythromycin production is still unclear

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  • Method for increasing erythromycin yield by transforming saccharopolyspora erythraea SACE_3980 gene
  • Method for increasing erythromycin yield by transforming saccharopolyspora erythraea SACE_3980 gene
  • Method for increasing erythromycin yield by transforming saccharopolyspora erythraea SACE_3980 gene

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Embodiment 1

[0030] 1.1 Strains, plasmids and growth conditions

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Abstract

The invention discloses a method for increasing the erythromycin yield by transforming the saccharopolyspora erythraea SACE_3980 gene. In saccharopolyspora erythraea, the gene SACE_3980 is subjected to transcription regulation by genetic engineering and through TetR-deficient family to obtain an engineering strain with high erythromycin yield; and by adopting the obtained strain for fermentation production of erythromycin, the yield can be remarkably increased, and a new technical support is provided for increasing the erythromycin yield in industrial production.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for improving the yield of erythromycin by modifying the gene of Saccharopolyspora erythromycetes SACE_3980. Background technique [0002] Erythromycin is produced by the secondary metabolism of Saccharopolyspora erythromycetes and is a typical polyketide antibiotic. Its components include erythromycin A (Er-A), erythromycin B (Er-B), erythromycin C (Er-C), erythromycin D (Er-D), erythromycin E (Er-E) and erythromycin F (Er-F), etc. This type of antibiotic has a broad-spectrum antibacterial effect, and its antibacterial spectrum is similar to that of penicillin, and has a strong inhibitory effect on Gram-positive bacteria. Among the various components of erythromycin, erythromycin A is widely used clinically, and its antibacterial activity is the highest, while chemical derivatives of erythromycin series (clarithromycin, azithromycin, roxithromycin, telithro...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12N1/15C12R1/645
CPCC07K14/37C12P19/62
Inventor 张部昌任少华汪焰胜吴杭王春宇王浩高娟子
Owner ANHUI UNIVERSITY
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