Method for increasing yield of erythromycin by transforming streptomyces erythraea SACE_0303 gene

A technology of Saccharopolyspora erythromycetes and erythromycin, applied in the field of genetic engineering, can solve the problems of large randomness, time-consuming, inability to provide theoretical guidance for breeding, etc.

Active Publication Date: 2019-08-23
ANHUI UNIVERSITY
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Problems solved by technology

Traditional mutagenesis techniques are not only time-consuming, but also highly random, and cannot provide theoretical guidance for breeding

Method used

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  • Method for increasing yield of erythromycin by transforming streptomyces erythraea SACE_0303 gene
  • Method for increasing yield of erythromycin by transforming streptomyces erythraea SACE_0303 gene
  • Method for increasing yield of erythromycin by transforming streptomyces erythraea SACE_0303 gene

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Embodiment Construction

[0021] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0022] The bacterial strains and plasmids used in the examples are shown in Table 1. Escherichia coli were cultured in liquid LB medium at 37°C or on solid LB plates supplemented with 1.25% agar. The erythromycin-producing erythromycin-producing erythromyces Saccharopolyspora wild strain A226 and its engineered strain WB were cultured in tryptone soy broth (TSBY) medium at 30°C or on MGM plates containing 2.2% agar.

[0023] Bacterial strains and plasmids used in the present embodiment of table 1

[0024]

[0025]

[0026]

[0027] In the examples, PEG3350, lysozyme, TES, casamino acids, thiostrepton, an...

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Abstract

The invention discloses a method for increasing the yield of erythromycin by transforming a streptomyces erythraea SACE_0303 gene. A positive regulator SACE_0303 is biosynthesized by utilizing the screened erythromycin, the TetR family transcriptional SACE_0303 gene in the streptomyces erythraea is overexpressed by means of genetic engineering, an erythromycin high-yield engineering strain of thestreptomyces erythraea is obtained, and fermented to produce the erythromycin, and technical support is provided for increasing the yield of the erythromycin through industrial production.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for improving the yield of erythromycin by modifying the gene of Saccharopolyspora erythromycetes SACE_0303. Background technique [0002] Actinomycetes can produce abundant secondary metabolites, and these secondary metabolites and their derivatives are widely used, such as antibiotics, anticancer drugs, immunosuppressants and so on. Erythromycin is a kind of macrolide antibiotic with broad-spectrum antibacterial effect produced by Saccharopolyspora erythromycetes. Since its discovery in 1952, erythromycin and its derivatives have been widely used clinically, and have now developed to the third generation. It can be seen that improving the output of erythromycin is an urgent problem to be solved at present. [0003] In the past, industrial production strains were mainly obtained by physical or chemical mutagenesis. Traditional mutagenesis techniques are not only ti...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12N15/80C12N15/90C12N15/31C12R1/645
CPCC12P19/62C12N15/80C12N15/902C07K14/37
Inventor 张部昌倪静姝张万祥吴杭汪焰胜
Owner ANHUI UNIVERSITY
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