Scopulariopsis brevicaulis and application thereof

A technology for short broom mold and mold is applied in the application field of degrading polycyclic aromatic hydrocarbons, and achieves the effect of eliminating environmental pollution and improving degradability.

Active Publication Date: 2014-04-16
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many microorganisms capable of degrading PAHs have been reported at home and abroad, but few have been successfully applied to soil remediation. This is mainly related to the properties of PAHs, the degradation ability of microorganisms, and the influence of various environmental factors.

Method used

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  • Scopulariopsis brevicaulis and application thereof
  • Scopulariopsis brevicaulis and application thereof
  • Scopulariopsis brevicaulis and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: PAH-degrading fungi (that is, the aforementioned Brachyphyllum ( Scopulariopsis brevicaulis )4Z, hereinafter also referred to as "strain 4Z") isolation, purification and identification.

[0025] 1. Culture medium: ①Martin's medium; ②Inorganic salt medium (MS): K per liter 2 HPO 4 6.0 g, KH 2 PO 4 5.5 g, NH 4 NO 3 2.0 g, Na 2 SO 4 2.0 g, KCl 2.0 g, MgSO 4 ?7H 2 O 0.2 g, trace metal salt solution 1.0 mL, distilled water, pH 7.0; ③ Inorganic salt medium containing PAHs: prepare 1000 mg / L of different PAHs (phenanthrene, benzo[a]pyrene, pyrene, fluoranthene) in DMF Solution, add sterilized inorganic salt medium according to the appropriate concentration.

[0026] 2. Isolation and purification of PAHs degrading bacteria

[0027] Weigh 10 g of fresh soil, add it to a Erlenmeyer flask filled with 100 mL of inorganic salt liquid medium (pyrene concentration: 100.0 mg / L), and shake at 28 °C for 1 week at 200 r / min. Transfer to fresh pyrene inorganic ...

Embodiment 2

[0033] Example 2: Degradation Characteristics of PAH Degrading Fungi to PAHs

[0034] 1. Under sterile conditions, inoculate the strain 4Z in Martin's liquid medium containing 100. mg / L polycyclic aromatic hydrocarbons, shake and culture at 200 r / min at 28°C for 1 week, collect the bacteria by centrifugation, and buffer with phosphate The solution was washed 3 times repeatedly, and then the bacterial suspension was adjusted to the required concentration with phosphate buffered saline for later use.

[0035] 2. Degradation of polycyclic aromatic hydrocarbons by bacterial strain 4Z: Add 10% of the bacterial suspension into 50 mL of liquid medium, take the treatment of inactivated bacterial suspension as a control, add the mother liquor of polycyclic aromatic hydrocarbons, and make phenanthrene, pyrene, and fluorescein The final concentration of anthracene was about 100 mg / L and that of benzo[a]pyrene was about 20 mg / L. Each treatment has 3 repetitions. Shake culture at 150 r...

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Abstract

The invention discloses Scopulariopsis brevicaulis and application thereof. A preservation serial number of Scopulariopsis brevicaulis 4Z is CGMCCNo.6870 (China General Microbiological Culture Collection Center). Scopulariopsis brevicaulis has a wider substrate spectrum, has good degradation ability on organic aromatic compounds, particularly polycyclic aromatic hydrocarbon in an environment, and can be applied to treatment of environmental pollution, for example, Scopulariopsis brevicaulis can act as a bioremediation reagent for soil polluted by the organic aromatic compounds for wide use.

Description

technical field [0001] The present invention particularly relates to a strain of Brachyphyton and its application in degrading polycyclic aromatic hydrocarbons. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of organic pollutants ubiquitous in the environment. Due to their long half-life in the environment and their carcinogenic, teratogenic, and mutagenic properties, people have paid attention to them. In the early 1980s, the U.S. Environmental Protection Agency identified 16 unbranched PAHs as priority pollutants in the environment, and my country also included them in the blacklist of environmental pollution. [0003] Microorganisms have a strong catabolic ability, and microbial degradation is the most important way to remove PAHs from the environment. In recent years, many microorganisms capable of degrading PAHs have been reported at home and abroad, but few have been successfully applied to soil remediation. This is mainly related to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B09C1/10A62D3/02C12R1/645A62D101/20
Inventor 毛健荆磊邓宗武
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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