In vitro molecular directed evolution method for reshaping antibody

A technology of molecular targeting and antibodies, applied in biochemical equipment and methods, using vectors to introduce foreign genetic material, botanical equipment and methods, etc., can solve the problems of verifying the degree of humanization of transplanted antibodies and modified antibodies
CN1566341AInactive Publication Date: 2005-01-19BEIJING ABT GENETIC ENG TECH +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING ABT GENETIC ENG TECH
Publication Date
2005-01-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an in vitro molecular directed evolution method for reshaping antibody, which integrates ribosome displaying technology with DNA reorganization technology by designing reshaped antibody molecules, thus directly obtaining the extracorporal molecules of the single-chain antibody reshaped orientation.
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Description

technical field

[0001] The present invention relates to an in vitro molecular directed evolution method for antibody reshaping, more specifically, the present invention relates to the development of murine single-chain antibody by designing reshaped antibody molecules and combining ribosome display technology and DNA shuffling technology. An in vitro molecular directed evolution method for antibody reshaping based on direct access to reshaped single-chain antibodies. The present invention also provides the amino acid sequence and its encoding nucleotide sequence of an anti-4-1BB modified antibody obtained by this method, the vector containing the nucleotide sequence and the host cell containing these vectors. Background technique

[0002] Compared with polyclonal antibodies, monoclonal antibodies have good specificity and higher affinity; they can be mass-produced with reproducibility; It is widely used in research and other fields. However, during application, especially ...

Claims

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