6 results about "Ribosome display" patented technology

The invention belongs to the technical field of antibody library construction, and particularly relates to a synthetic nano library for ribosome display screening and construction and application thereof. Aiming at the problems that the existing nano antibody acquisition depends on animal immunity, the diversity and screening efficiency of a synthetic library are insufficient and the like, the invention discloses a synthetic nano antibody library suitable for ribosome display and a construction method of the synthetic nano antibody library, and the synthetic nano antibody library suitable for ribosome display is constructed by combining a trinucleotide primer synthesis and Type IIS enzyme digestion connection technology through a consensus skeleton design and a CDR region differentiation randomization strategy. The library stability and the effective diversity are improved. The library does not need to immunize animals, is short in period and low in cost, is compatible with toxic antigens, can be screened through combination of ribosome and bacteriophage, can efficiently obtain nano-mole-level affinity nano-antibodies, can also assist structure research of a cryoelectron microscope, has the advantages of being complete in library, efficient in screening, low in sequence redundancy and the like, and provides powerful support for research and development of biological medicines.

The application discloses a dual-epitope llama-derived nanobody and application thereof, and belongs to the field of biological medicine. The application is aimed at different epitopes of MERS-CoV, and two strains of antibodies 2-77 and 4-58 aiming at different binding sites of MERS-CoV RBD are obtained by immunizing a llama, performing specific screening on a ribosome display nanobody library, performing a pseudovirus neutralization experiment, performing a competitive BLI experiment, identifying VHH epitopes and molecular docking by using a phage random peptide library, and constructing a dual-epitope llama-derived nanobody 2F4 with good neutralization activity, high expression and stability by using the two strains of nanobodies. It is proved by a lesion formation test and a mouse in-vivo experiment that the dual-epitope llama-derived nanobody 2F4 constructed by the application can effectively neutralize MERS-CoV and effectively protect animals, and the application provides a train of thought and technical support for exploring new antigen epitopes of MERS-CoV and designing a therapeutic antibody drug.

The application discloses a nano antibody specifically recognizing staphylococcus aureus and a preparation method and application thereof. The ribosome display technology is adopted to screen a pair of nano antibodies with good pairing effect through construction of a nano antibody display library, three rounds of high-throughput screening and ELISA verification, and the nano antibody is combined with an immunochromatography technology to be used for in-vivo detection of actual pathogenic bacteria. The nano antibody disclosed by the application shows high affinity and high specificity when being combined with the staphylococcus aureus, can realize a detection limit, and has the advantages of good stability and low cost compared with a monoclonal antibody, can be produced on a large scale, and has a wide application prospect in the field of staphylococcus aureus detection and analysis.

This invention provides a nanobody targeting canine OSMRβ receptor M and its applications, belonging to the field of nanobody preparation technology. This invention achieves recombinant expression and purification of the canine OSMRβ receptor, and obtains the targeting nanobody HX016 based on ribosome display technology. Enzyme-linked immunosorbent assay (ELISA) confirms that the nanobody HX016-cFc specifically binds to canine OSMRβ. SPR technology confirms that the nanobody HX016-cFc exhibits good affinity for canine OSMRβ. Cell-level experiments show that the nanobody HX016-cFc can effectively bind to canine OSMRβ and significantly inhibit IL-31-induced downstream signaling pathway activation in a dose-dependent manner, thereby effectively intervening in the IL-31-driven itching and inflammatory response in canine atopic dermatitis at the mechanistic level. A mutated portion of HX016 also possesses the corresponding ability to bind to the canine OSMRβ antigen.

The application discloses a nano antibody specifically recognizing staphylococcus aureus and a preparation method and application thereof. The ribosome display technology is adopted to screen a pair of nano antibody with good pairing effect through construction of a nano antibody display library, three rounds of high-throughput screening and ELISA verification, and the nano antibody is combined with an immunochromatography technology to be used for in-vivo detection of actual pathogenic bacteria. The nano antibody disclosed by the application shows high affinity and high specificity when being combined with the staphylococcus aureus, can realize a detection limit, and has the advantages of good stability and low cost compared with a monoclonal antibody, can be produced on a large scale, and has a wide application prospect in the field of staphylococcus aureus detection and analysis.

The application discloses a nano antibody specifically recognizing staphylococcus aureus and a preparation method and application thereof. The ribosome display technology is adopted to screen a pair of nano antibodies with good pairing effect through construction of a nano antibody display library, three rounds of high-throughput screening and ELISA verification, and the nano antibody is combined with an immunochromatography technology to be used for in-vivo detection of actual pathogenic bacteria. The nano antibody disclosed by the application shows high affinity and high specificity when being combined with the staphylococcus aureus, can realize a detection limit, and has the advantages of good stability and low cost compared with a monoclonal antibody, can be produced on a large scale, and has a wide application prospect in the field of staphylococcus aureus detection and analysis.