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1 results about "Ribosome display" patented technology
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Ribosome display is a technique used to perform in vitro protein evolution to create proteins that can bind to a desired ligand. The process results in translated proteins that are associated with their mRNA progenitor which is used, as a complex, to bind to an immobilized ligand in a selection step. The mRNA-protein hybrids that bind well are then reverse transcribed to cDNA and their sequence amplified via PCR. The end result is a nucleotide sequence that can be used to create tightly binding proteins.
A nanobody against canine oncostatin m receptor beta and applications thereof
PendingCN122356288AOncostatin M Receptor betaImmunosorbent technique
This invention provides a nanobody targeting canine OSMRβ receptor M and its applications, belonging to the field of nanobody preparation technology. This invention achieves recombinant expression and purification of the canine OSMRβ receptor, and obtains the targeting nanobody HX016 based on ribosome display technology. Enzyme-linked immunosorbent assay (ELISA) confirms that the nanobody HX016-cFc specifically binds to canine OSMRβ. SPR technology confirms that the nanobody HX016-cFc exhibits good affinity for canine OSMRβ. Cell-level experiments show that the nanobody HX016-cFc can effectively bind to canine OSMRβ and significantly inhibit IL-31-induced downstream signaling pathway activation in a dose-dependent manner, thereby effectively intervening in the IL-31-driven itching and inflammatory response in canine atopic dermatitis at the mechanistic level. A mutated portion of HX016 also possesses the corresponding ability to bind to the canine OSMRβ antigen.
Owner:CHINA AGRI UNIV