12 peptide ZA for inhibiting hepatitis c virus to infect human cell and preparation method and application

An E2-GST, BL21DE3 technology, applied in antiviral agents, peptides, pharmaceutical formulations, etc., can solve the problems of adverse reactions, individual differences of patients, and limited dosage of drugs
CN101456901AInactive Publication Date: 2009-06-17WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHAN UNIV
Publication Date
2009-06-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a dodecapeptide ZA capable of inhibiting the hepatitis C virus from infecting human body cells and a preparation method and application thereof. The preparation method comprises the following steps: firstly preparing an E2-GST fusion protein; then preparing an E2-GST fusion protein antibody; thirdly establishing a random DNA library; fourthly preparing a ribosome library; and fifthly screening the polypeptide bonded with the hepatitis C virus E2 glycoprotein through the ribosome display library so as to obtain the dodecapeptide (ZA) specially bonded with the E2 protein. Due to the high affinity of the polypeptide ZA with the hepatitis C virus E2 glycoprotein, the polypeptide ZA can remarkably alleviate the intrusion of HCV into the human body cells and DC cells, inhibit the integration of the E2 protein with human body cells, and have a high inhibition capacity. The dodecapeptide ZA is used for preparing the medicines capable of treating and preventing hepatitis C virus infection. The polypeptide is applied to a kit for detecting the hepatitis C virus envelope antigen.
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Description

technical field

[0001] The invention belongs to the field of molecular biology of viruses. More specifically, it relates to a 12-peptide ZA that inhibits hepatitis C virus from infecting human cells, and also relates to inhibiting the binding of hepatitis C virus to human hepatocytes and DC-SIGN + The preparation method of the polypeptide of the target cell, and also relates to a small molecule 12 peptide capable of targeting the envelope glycoprotein E2 of hepatitis C virus, that is, the use of polypeptide ZA in the preparation of drugs for treating or preventing hepatitis C virus from infecting human cells , the polypeptide is also used as a kit for detecting hepatitis C virus envelope antigen. Background technique

[0002] Hepatitis C virus (hepatitis C for short) is an inflammatory disease of the liver caused by hepatitis C virus (Hepatitis C Virus, HCV). HCV infection has a global distribution and is mainly transmitted through blood. According to the World Health Org...

Claims

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