12 peptide ZB for inhibiting hepatitis c virus to infect human cell and preparation method and application

A technology of E2-GST and BL21DE3, applied in antiviral agents, peptides, pharmaceutical formulations, etc., can solve the problems of individual patient differences, adverse reactions, and limited drug doses
CN101456900AInactive Publication Date: 2009-06-17WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHAN UNIV
Publication Date
2009-06-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a dodecapeptide ZB capable of inhibiting the hepatitis C virus from infecting human body cells and a preparation method and application thereof. The preparation method comprises the following steps: firstly preparing an E2-GST fusion protein; then preparing an E2-GST fusion protein antibody; thirdly establishing a random DNA library; fourthly preparing a ribosome library; and fifthly screening the polypeptide bonded with the hepatitis C virus E2 glycoprotein through the ribosome display library so as to obtain the polypeptide specially bonded with the E2 protein. Due to the high affinity of the polypeptide ZB with the hepatitis C virus E2 glycoprotein, the polypeptide ZB can remarkably alleviate the intrusion of HCV into the human body cells, inhibit the binding force of HCV with human body hepatocytes, have a high inhibition capacity, and competitively inhibit the integration of a virus HCVE2 and a receptor CD81 after having an action with the E2 protein. The dodecapeptide is used for preparing the medicines capable of treating and preventing hepatitis C virus infection. In addition, the polypeptide is applied to a kit for detecting the hepatitis C virus envelope antigen.
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Description

technical field

[0001] The invention belongs to the field of molecular biology of viruses. More specifically, it relates to a 12-peptide ZB that inhibits hepatitis C virus from infecting human cells, and also relates to inhibiting the binding of hepatitis C virus to human hepatocytes and DC-SIGN + The preparation method of the polypeptide ZB of the target cell of the present invention also relates to a small molecule 12 peptide capable of targeting the envelope protein E2 of hepatitis C virus, that is, the use of the polypeptide ZB in the preparation of drugs for treating or preventing hepatitis C virus from infecting human cells , the polypeptide is also used as a kit for detecting hepatitis C virus envelope antigen. Background technique

[0002] Hepatitis C virus (hepatitis C for short) is an inflammatory disease of the liver caused by hepatitis C virus (Hepatitis C Virus, HCV). HCV infection has a global distribution and is mainly transmitted through blood. According t...

Claims

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