The application discloses a water-soluble AIE fluorescent probe, and a structure formula is shown in the following formula (I), wherein R1 and R2 are independently selected from
alkyl alcohol with
carbon number of 1-6; X is selected from water-soluble anions, and M is selected from water-soluble cations. The fluorescent probe has AIE characteristics, can realize rapid and efficient
staining of cytoplasmic membranes, and does not need to be cleaned; the fluorescent probe has good water
solubility, small
cytotoxicity, and can be used in
in vivo cell fluorescence imaging; the fluorescent probe has orange
red fluorescence color, large
Stokes shift, and strong anti-self-
fluorescence interference capability.