42 results about "Cell plasma membrane" patented technology

The present invention relates to a novel in vitro assay for determining the level of a protein, in particular, a membrane transport protein that is located at the plasma membrane of a cell compared to the level of the protein in the cell. The process of the invention is also useful for determining the level of recycling of a membrane transport protein. The present invention additionally provides a process for identifying an agent that modulates the translocation of a protein, in particular, a membrane transport protein, to the plasma membrane and, as a consequence, the activity of that protein.

The present invention relates to antibodies or antibody fragments that may be displayed on the extracellular surface of the plasma membrane when expressed in a host cell. The present invention provides libraries comprising a plurality of plasma membrane displayed antibodies and methods of screening the libraries for antibodies or antibody fragments with desired characteristics.

Liposomes containing therapeutic genes are conjugated to multiple targeting agents to provide transport of the encapsulated gene across the blood-retinal barrier and the plasma membrane of ocular cells. Once across the blood-retinal barrier and ocular cell membrane, the encapsulated gene expresses the encoded therapeutic agent within the ocular cells to provide diagnosis and / or treatment of disease.

The present invention relates to an antigen presenting membrane vesicle comprising on its surface a composition of either relevant molecules for antigen-specific activation or deactivation of T lymphocytes and which is present in the form of an artificially induced lipid vesicle budded from a plasma membrane of a eukaryotic, preferably human, cell, and wherein the composition of said relevant molecules for activation or deactivation present on the vesicle surface is adjustable to a recipient's needs or requirements independently of any blood or tissue cells of said recipient. The invention further relates to a method of manufacture of the vesicles and to compositions containing the vesicles as well as to the use of the vesicles for various purposes including medical and diagnostic applications.

A fluorescent probe for marking and tracking cytoplasmic membranes and a preparation method of the fluorescent probe belong to the field of fine chemical engineering. According to the fluorescent probe, BODIPY is adopted as a substrate to react with carbazole aldehyde under nitrogen or argon protection, piperidine is adopted as a catalyst, and a 4-angstrom molecular sieve is adopted as a dehydrating agent; the reaction product reacts with a quaternization reagent to obtain fluorescent probe molecules. The fluorescent probe has a large emission wave length ranging from 600 to 700 nm, avoids the interference of autofluorescence of cells and reduces the optical damage on cells; the fluorescent probe has a bipolar structure, fluorescence quenching is caused when the fluorescent probe congregates in water, and the fluorescence is obviously improved when the fluorescent probe is embedded in the cytoplasmic membranes; the probe participates in biological processes together when positioned in the cytoplasmic membrane, and being free from toxic and side effects, the probe is suitable for microscopic observation of cellular processes related to the cytoplasmic membranes; the fluorescent probe has high synthesis efficiency and stable properties, is easy to preserve for a long term, and provides a visual and concise low-cost detection reagent for studying relevant processes of the cytoplasmic membranes.

The invention describes novel reagents that can be applied for analysis of the quality of human cells. The method evaluates the integrity of the plasma membrane of the cells by detecting novel glyco structures found only intracellularly. The method can be applied, for example, to demonstrate exposure of therapeutic cell preparation to potentially harmful conditions. It can also be used as a quality control tool in methods in which intact cell membrane is essential and it can be applied in separation of damaged cells from non- damaged.

The present invention relates generally to methods for determining the effectiveness of ongoing antidepressant therapy via analysis of the association of Gsα with components of the plasma membrane or cytoskeleton of cells from peripheral tissues of the depressed individual as well as to methods involved in screening for effective antidepressant agents via their ability to cause a difference in the association of Gsα with components of the plasma membrane or cytoskeleton of cells.

The invention discloses a group of fluorescent probes for simultaneously displaying cell nucleus structure and cell integral morphology in live cells. The group of fluorescent probes comprises a generalized RNA (ribonucleic acid) fluorescent probe with a chemical structure shown in a formula (I), a cell nucleus fluorescent probe with a chemical structure shown in a formula (II), and a cell membrane fluorescent probe with a chemical structure shown in a formula (III). The invention also discloses application of the group of fluorescent probes for labeling or displaying cell integral morphology, especially labeling or displaying the cell nucleus structure including nucleolus distribution. Proofed by experiment, the group of fluorescent probes has the characteristics that the application range is broad, the membrane permeability is good, the cell toxicity is low, and the cell nucleus, cell nucleolus, cell plasma and cell plasma member can be simultaneously imaged; the application prospect is broad.

The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.

An organ protectant solution which is intravenously administerable includes a high concentration of cell impermeant molecules which have a charge and / or molecular weight which permit passage across a capillary endothelium and into an interstitial space in said subject but which are too large and / or charged to cross a cell plasma membrane such that said one or more cell impermeant molecules preferentially load into an extracellular fluid compartment can be used to to allow for improved organ harvesting from DCD and brain death donors for transplantation purposes and also can be used extend the “Golden Hour” for traumatic and hemorrhagic shock patients thereby allowing more time for those patients to reach a point of care facility to receive medical treatment.

The invention provides a tangerine extract composition for feminite care, which is composed of the following components in parts by weight: 0.2 to 18 parts of hesperidin, 0.04 to 1.24 parts of neohesperidin, 0.015 to 0.86 parts of naringin, 0.01 to 0.5 parts of limonin, 0.05 to 2.5 parts of vitamin P, 0.03 to 0.85 parts of hesperetin and 0.25 to 13.8 parts of vitamin C. The tangerine extract composition for feminite care provided by the invention has the following technical effects: the synergy of the components is realized, the hesperidin, the neohesperidin, the naringin, the limonin, the vitamin P, the hesperetin and the vitamin C are jointly functioned on preventing the cell plasma membranes from absorbing an ammonia acid and blocking the cell respiration, so that the effect on damaging the microbial cell membranes is achieved, and the effect on effectively killing the harmful microorganisms including bacteria, fungi, viruses and parasites and the like is realized. The tangerine extract composition is a natural physical sterilizing product in a real sense.

Cell plasma membrane impermeable compounds and compositions thereof used for anticancer treatment are provided. The compounds contain functional groups capable of blocking and / or inhibiting trans-plasma membrane electron transport (tPMET), cell surface respiration and uncoupling cell surface oxidative-phosphorylation. The compositions comprise at least one of the cell plasma membrane impermeable compounds in combination with compounds of multi-function inhibitor capable of inhibiting cancer cell hypoxia responses.

The invention relates in some aspects to methods and kits for determining plasma membrane repair status in cells, tissues, and subjects. The methods include, in part, the use of high-throughput assays for assessing cell plasma membrane repair status. The methods and kits of the invention are useful to identify compounds that enhance or inhibit plasma membrane repair and to identify plasma membrane repair-associated disorders and diseases in cells, tissues, and subjects. The methods of the invention are also useful to select treatments, monitor treatment efficacy, and to assess the onset, progression, and / or regression of plasma membrane repair-associated disorders and diseases.

Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.

The invention relates to the technical field of biological pharmaceuticals, in particular to a fusion protein and an application thereof. According to the fusion protein, a plasma membrane positioningsequence, a Dab1 protein, a light-sensitive protein and a fluorescent protein are in fusion to be positioned to a lipid raft region or a non lipid raft region of a cytoplasmic membrane, and through illumination, quick activation and adjustment and control of a PI3K / Akt signal channel can be realized locally at a high time and space resolution. The fusion protein has great important significance in quick effective specific opening and closing control of the signal channel, contributing to understanding a cascade transmission rule of signals and researching diseases closely related to the signal channel.

The present invention relates to a method for screening a modulator of a TMEM16 family member, which comprises the following steps:(1) treating cells expressing the TMEM16 family member with a candidate of the modulator, and(2) determining whether the candidate alters distribution of a lipid selected from phosphatidylserine, phosphatidylcholine, and galactosylceramide in plasma membrane of the cells,wherein a candidate which increases distribution of phosphatidylserine in the outer leaflet of plasma membrane compared to control is selected as a modulator enhancing a function of the TMEM16 family member, and a candidate which decreases distribution of phosphatidylserine in the outer leaflet of plasma membrane compared to control is selected as a modulator suppressing a function of the TMEM16 family member, anda candidate which increases distribution of phosphatidylcholine or galactosylceramide in the inner leaflet of plasma membrane compared to control is selected as a modulator enhancing a function of the TMEM16 family member, and a candidate which decreases distribution of phosphatidylcholine or galactosylceramide in the inner leaflet of plasma membrane compared to control is selected as a modulator suppressing a function of the TMEM16 family member.

The invention provides special fertilizer for iron-rich lily. According to growth and development characteristics as well as nutritional characteristics at each growth and development stage of edible and medicinal lily, elements nitrogen, phosphor, potassium and iron in certain proportion are prepared into an aqueous solution in a mode of foliage top-dressing and leaf spraying; then, the aqueous solution is sprayed on the growing lily leaves, so that the fertilizer reaches epidermic cell plasma membranes through leaf cuticular membranes via ectodesmata to enter plants, and therefore, development of lily bulbs is regulated, the yield is increased and content of the functional element iron in the bulbs is increased; compared with root fertilizer application, the application method can effectively avoid loss of nutrients, reduces dose of the fertilizer, is beneficial for protecting the environment and increases a fertilizer utilization rate. The special fertilizer is simple in preparation process, and is suitable for large-scale popularization.

A Na<+> / H+ antiporter gene on a wheat plasma membrane and applications thereof are disclosed. The full length of the antiporter gene is 3460 bp. The gene after expression is positioned on a cell plasma membrane, and exerts a Na<+> / H+ exchange function to discharge Na<+> out of cells. A method of constructing a plant genetic transformation vector by utilizing the antiporter gene and improving plant salt tolerance is also disclosed.

Fusion of the membrane of enveloped viruses with the plasma membrane of a receptive host cell is a prerequisite for viral entry and infection and an essential step in the life cycle of all enveloped viruses, such as paramyxoviruses. The instant invention is directed to providing polypeptides which are a heptad portion of a Henipavirus F protein effective against fusion between a membrane of a paramyxovirus and a plasma membrane of a cell. The instant invention also provides nucleic acids, compositions, and methods effective against paramyxovirus infection. Accordingly, the instant invention provides therapeutic agents and vaccines effective against paramyxoviruses viruses, especially HeV or NiV.

3-hydroxyflavone derivatives are useful in the detection and the quantification of cell apoptosis. Such derivatives are also useful as fluorescent probes in studying lipid asymmetry of cell plasma membrane and in detecting apopoptic cells. The derivatives can be used to monitor the evolution of diseases involving cell apoptosis.

An organ protectant solution which is intravenously administerable includes a high concentration of cell impermeant molecules which have a charge and / or molecular weight which permit passage across a capillary endothelium and into an interstitial space in said subject but which are too large and / or charged to cross a cell plasma membrane such that said one or more cell impermeant molecules preferentially load into an extracellular fluid compartment can be used to to allow for improved organ harvesting from DCD and brain death donors for transplantation purposes and also can be used extend the “Golden Hour” for traumatic and hemorrhagic shock patients thereby allowing more time for those patients to reach a point of care facility to receive medical treatment.

The invention provides a peptide chain modified tetraphenylethylene derivative and a preparation method and application thereof, and belongs to the technical field of fluorescence imaging. TPE or a derivative thereof is marked on a polypeptide chain, the solubility and cell permeability of hydrophobic AIE molecules are improved, the obtained peptide chain modified tetraphenylethylene derivative isgood in water solubility, biocompatibility and selective permeability of a cytoplasmic membrane, a typical aggregation-induced emission (AIE) behavior is shown, dyeing imaging of a cytoplasmic regioncan be achieved, and the derivative can serve as a fluorescence probe molecule to be applied to the field of cell fluorescence imaging. The polypeptide chain is adopted as a modifying group, and thederivative has excellent biocompatibility and has great advantages compared with inorganic and organic nanoparticles of a polymer matrix or a silicon matrix with biotoxicity in the prior art.

The present invention provides for testing methods to determine an effective testing agent that affects the activity of the HIV Gag protein at the plasma membrane of a cell, and specifically, effecting changes in the structural conformation of at least one fatty acid of PI(4,5)P2, a member of a family of differentially phosphorylated phosphatidylinositides, wherein inhibition of the extension of such fatty acid into the MA domain of the Gag protein reduces binding of Gag to the plasma membrane, thereby inhibiting virus particle assembly and subsequent replication of the HIV virus.

The present invention relates to a method for increasing the permeability of a cytoplasmic membrane by directing at least one cell onto or near a structure comprising particles capable of absorbing electromagnetic radiation. The particles are present at a concentration of 0.001 to 20 vol% and are embedded in the material of the structure. At least 60% of the particles present in the structure are embedded in the material such that the shortest distance L between these particles and the free region surface S of the structure is 1 nm to 500 nm. The invention also relates to a structure suitable for the permeabilization of cells in photo-thermal processes and to the use of said structure.

The invention relates to novel multi-modality probes for imaging, tracking and analyzing stem cells and related biological samples, and methods of preparation and use thereof. The molecular probes of the invention are constructed, for example, by utilizing (a) the high selectivity of long hydrocarbon chains for binding to plasma membranes of cells, (b) a near-infrared (NIR) dye for optical imaging, and (c) a radionuclide for PET or SPECT imaging. The in vitro and in vivo data of the optical and radiolabeled probes demonstrated their utility for detecting the presence of stem cells with multiple imaging modalities.

The invention belongs to the technical field of biomedicine, and discloses an isomer for expressing specific STING, a cell line, a preparation method and application, the STING isomer comprises erSTING and pmSTING, and Pre-mRNA transcribed by an STING gene has two transcripts with different splicing modes; the longer transcript is translated and processed into classical erSTING to be positioned on the endoplasmic reticulum; the short transcript lacks a transmembrane region due to exon jumping, and is translated and processed into pmSTING to be positioned on a cytoplasmic membrane. According to the invention, mouse and human cell lines expressing specific STING isomers are constructed for the first time, namely, a B16 cell line only expressing erSTING and only expressing pmSTING and an HEK293T cell line only expressing erSTING and only expressing pmSTING, and a foundation is laid for research and development of novel micromolecular immunotherapy drugs taking STING as a target spot.