39 results about "Cell plasma membrane" patented technology

A fluorescent probe for marking and tracking cytoplasmic membranes and a preparation method of the fluorescent probe belong to the field of fine chemical engineering. According to the fluorescent probe, BODIPY is adopted as a substrate to react with carbazole aldehyde under nitrogen or argon protection, piperidine is adopted as a catalyst, and a 4-angstrom molecular sieve is adopted as a dehydrating agent; the reaction product reacts with a quaternization reagent to obtain fluorescent probe molecules. The fluorescent probe has a large emission wave length ranging from 600 to 700 nm, avoids the interference of autofluorescence of cells and reduces the optical damage on cells; the fluorescent probe has a bipolar structure, fluorescence quenching is caused when the fluorescent probe congregates in water, and the fluorescence is obviously improved when the fluorescent probe is embedded in the cytoplasmic membranes; the probe participates in biological processes together when positioned in the cytoplasmic membrane, and being free from toxic and side effects, the probe is suitable for microscopic observation of cellular processes related to the cytoplasmic membranes; the fluorescent probe has high synthesis efficiency and stable properties, is easy to preserve for a long term, and provides a visual and concise low-cost detection reagent for studying relevant processes of the cytoplasmic membranes.

The invention describes novel reagents that can be applied for analysis of the quality of human cells. The method evaluates the integrity of the plasma membrane of the cells by detecting novel glyco structures found only intracellularly. The method can be applied, for example, to demonstrate exposure of therapeutic cell preparation to potentially harmful conditions. It can also be used as a quality control tool in methods in which intact cell membrane is essential and it can be applied in separation of damaged cells from non- damaged.

A peptide directs nanoparticles (such as quantum dots) to the plasma membrane of mammalian cells. A method of delivery of a nanoparticle to a plasma membrane of a cell includes providing to the cell a nanoparticle attached to a peptide configured to direct the nanoparticle the plasma membrane, and allowing the cell to take up the nanoparticle. The nanoparticle can be a FRET donor to an organic dye.

The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.

The invention provides a tangerine extract composition for feminite care, which is composed of the following components in parts by weight: 0.2 to 18 parts of hesperidin, 0.04 to 1.24 parts of neohesperidin, 0.015 to 0.86 parts of naringin, 0.01 to 0.5 parts of limonin, 0.05 to 2.5 parts of vitamin P, 0.03 to 0.85 parts of hesperetin and 0.25 to 13.8 parts of vitamin C. The tangerine extract composition for feminite care provided by the invention has the following technical effects: the synergy of the components is realized, the hesperidin, the neohesperidin, the naringin, the limonin, the vitamin P, the hesperetin and the vitamin C are jointly functioned on preventing the cell plasma membranes from absorbing an ammonia acid and blocking the cell respiration, so that the effect on damaging the microbial cell membranes is achieved, and the effect on effectively killing the harmful microorganisms including bacteria, fungi, viruses and parasites and the like is realized. The tangerine extract composition is a natural physical sterilizing product in a real sense.

Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.

The present invention relates to a method for screening a modulator of a TMEM16 family member, which comprises the following steps:

• (1) treating cells expressing the TMEM16 family member with a candidate of the modulator, and
• (2) determining whether the candidate alters distribution of a lipid selected from phosphatidylserine, phosphatidylcholine, and galactosylceramide in plasma membrane of the cells,

wherein a candidate which increases distribution of phosphatidylserine in the outer leaflet of plasma membrane compared to control is selected as a modulator enhancing a function of the TMEM16 family member, and a candidate which decreases distribution of phosphatidylserine in the outer leaflet of plasma membrane compared to control is selected as a modulator suppressing a function of the TMEM16 family member, and

a candidate which increases distribution of phosphatidylcholine or galactosylceramide in the inner leaflet of plasma membrane compared to control is selected as a modulator enhancing a function of the TMEM16 family member, and a candidate which decreases distribution of phosphatidylcholine or galactosylceramide in the inner leaflet of plasma membrane compared to control is selected as a modulator suppressing a function of the TMEM16 family member.

A Na<+>/H+ antiporter gene on a wheat plasma membrane and applications thereof are disclosed. The full length of the antiporter gene is 3460 bp. The gene after expression is positioned on a cell plasma membrane, and exerts a Na<+>/H+ exchange function to discharge Na<+> out of cells. A method of constructing a plant genetic transformation vector by utilizing the antiporter gene and improving plant salt tolerance is also disclosed.

The invention provides a peptide chain modified tetraphenylethylene derivative and a preparation method and application thereof, and belongs to the technical field of fluorescence imaging. TPE or a derivative thereof is marked on a polypeptide chain, the solubility and cell permeability of hydrophobic AIE molecules are improved, the obtained peptide chain modified tetraphenylethylene derivative isgood in water solubility, biocompatibility and selective permeability of a cytoplasmic membrane, a typical aggregation-induced emission (AIE) behavior is shown, dyeing imaging of a cytoplasmic regioncan be achieved, and the derivative can serve as a fluorescence probe molecule to be applied to the field of cell fluorescence imaging. The polypeptide chain is adopted as a modifying group, and thederivative has excellent biocompatibility and has great advantages compared with inorganic and organic nanoparticles of a polymer matrix or a silicon matrix with biotoxicity in the prior art.

The invention belongs to the technical field of biomedicine, and discloses an isomer for expressing specific STING, a cell line, a preparation method and application, the STING isomer comprises erSTING and pmSTING, and Pre-mRNA transcribed by an STING gene has two transcripts with different splicing modes; the longer transcript is translated and processed into classical erSTING to be positioned on the endoplasmic reticulum; the short transcript lacks a transmembrane region due to exon jumping, and is translated and processed into pmSTING to be positioned on a cytoplasmic membrane. According to the invention, mouse and human cell lines expressing specific STING isomers are constructed for the first time, namely, a B16 cell line only expressing erSTING and only expressing pmSTING and an HEK293T cell line only expressing erSTING and only expressing pmSTING, and a foundation is laid for research and development of novel micromolecular immunotherapy drugs taking STING as a target spot.