Plant strong salt-resistant gene AtNHXS1 and its coding protein and application

Abstract

The invention provides a novel plant strong salt tolerant gene AtNHXS1 acquired through DNA reshuffling technology, and the ionic transport activity of Na+ / H+ antiporter protein coded by the gene is stronger than that of wild Na+ / H+ antiporter protein ATNHX1. A nucleotide sequence of the gene as shown in SEQ ID NO:1 also comprises genes of the nucleotide sequence of sequence 1 in a sequence tablewith the homology between 70 and 100 percent, or a nucleotide sequence of an amino acid sequence of sequence 2 in a coded sequence table. The new Na+ / H+ antiporter has a protein of the amino acid sequence of sequence 2 in the sequence table, protein of the amino acid sequence of the sequence 2 with the homology between 70 and 100 percent, or protein which replaces, deletes or adds one or a plurality of amino acids in the amino acid sequence of the sequence 2 with the same homology. The invention also provides the construction of recombinant vector, the transgenic plant and other methods so asto apply the gene and the protein, thereby cultivating a novel variety of transgenic plant with strong capacity of salt tolerance or other improved biologic characters.

Description

A Novel Plant Strong Salt Tolerance Gene AtNHXS1, Its Encoded Protein and Its Application technical field The present invention relates to the field of plant genetic engineering, in particular to using DNA shuffling technology to obtain the gene of plant Na+ / H+ antiporter with significantly improved function, and also relates to using the gene to cultivate transgenic salt-tolerant plants. Background technique Soil salinization is an important abiotic stress factor affecting crop production and ecological environment. At present, about 20% of the cultivated land and nearly 50% of the irrigated land in the world are seriously harmed by salinity (Flowler TJ, Yeo AR. Breeding for salinity resistance in cropplants. Where next? Aust. J. Plant Physiol. 1995, 22: 875-884). There are about 500 million mu of saline-alkali land in my country, and its area has a tendency to increase continuously. Cultivating salt-tolerant plants is an effective way to promote the development and util...

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Problems solved by technology

However, the application of this technology in improving the performance of the Na+ / H+ antiporter gene and its protein has not been reported at home and abroad.

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