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CBF transcription factor capable of regulating plant stress resistance, and coded gene and application thereof

A transcription factor and gene technology, applied in the field of transcription factors related to plant stress tolerance, can solve problems such as lack of gene regulation

Inactive Publication Date: 2011-09-21
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Ilex is mainly on the physiological and biochemical level, and there is no report on gene regulation

Method used

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  • CBF transcription factor capable of regulating plant stress resistance, and coded gene and application thereof
  • CBF transcription factor capable of regulating plant stress resistance, and coded gene and application thereof
  • CBF transcription factor capable of regulating plant stress resistance, and coded gene and application thereof

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Experimental program
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Effect test

Embodiment

[0059] 1.1 Experimental materials

[0060] 1.1.1 Plant material

[0061] The plant material is Ilex mongolica seedlings, and the seeds are provided by researcher Pei Xinwu from the Institute of Biotechnology, Chinese Academy of Agricultural Sciences. Soak the seeds in warm water at 50-60°C for 24 hours, remove the grains, soak in cold water for 1 day and night, and change the water 2-3 times during the period. After imbibition, remove the seeds to 0.5% KMnO4 solution, soak them for 30 minutes, wash them with water, put them into a seedling incubator, and cover them with gauze. Spray water on the gauze 2 to 3 times a day, turn the seeds frequently to make them germinate evenly, keep the temperature at 20-25°C, and all germination will be completed in 4-6 days. Select the seeds to sow in seedling containers, 2 to 3 seeds per pot, cover with 1 to 1.5 cm of vermiculite, replenish water appropriately, and the seedlings will emerge after 7 to 10 days. Put the seedlings of Ilex ja...

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Abstract

The invention discloses a CBF transcription factor capable of regulating plant stress resistance, and a coded gene and application thereof. A CBF full-length gene is cloned and identified from Ammopiptanthus Mongolicus by an RACE strategy; a semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) test proves that the gene is induced by low temperature to start to express in the first hour after cold treatment and have the highest expression quantity after two hours. According to bioinformatics analysis, AmCBF gene coded protein has an AP2 structure domain and a conservative characteristic polypeptide sequence in a CBF family and belongs to a CBF gene family in an AP2 / EREBP transcription factor family. Compared with wild type tobaccos, the AmCBF gene-transcribed tobacco leaves after cold treatment obviously reduce percolation ratio of electrolyte and obviously increase proline content and soluble sugar content, so the cold resistance is improved. A drought tolerance test shows that the drought tolerance of the transgenic tobaccos is greatly improved. The test result shows that the AmCBF can respond adversity signals and can play a role.

Description

technical field [0001] The present invention relates to the CBF transcription factor related to plant stress resistance and its coding gene, especially relates to the CBF transcription factor gene and its coding protein for regulating plant stress resistance isolated from Ammopiptanthus mongolicus. The invention relates to their application in improving plant stress resistance, and belongs to the field of transcription factors related to plant stress tolerance. Background technique [0002] Low temperature and drought are global abiotic stress factors that affect plant growth and limit crop yield. Low temperature and cold damage is a serious natural disaster in agricultural production, which causes huge losses in the world every year, which is estimated to reach 200 billion U.S. dollars. The research on the mechanism of plant cold resistance is not only of great significance in theory, but also has extensive application value in production. The study of low temperature dam...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/63C12N15/82C12N1/15C12N1/19C12N1/21C12N5/10C07K14/415A01H5/00
Inventor 程红梅郭惠明张晗
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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