Tissue culture method of ammopiptanthus mongolicus

Abstract

The invention provides a tissue culture method of ammopiptanthus mongolicus. Embryos in ammopiptanthus mongolicus seeds are taken as explants, through primary inducing culture, subculture, rooting culture and transplantation, selection of ammopiptanthus mongolicus explants, the optimal culture condition, optimal culture mediums at different stages, and the compounding ratio of cultivation substrates are systematically researched. Through experiments, the optimal culture mediums at different stages and the combination of the culture mediums in tissue culture of the ammopiptanthus mongolicus areobtained, through the optimal culture condition and the culture manner are obtained, the browning problem in the tissue culture process of the ammopiptanthus mongolicus is solved, the survival rate of tissue culture seedlings is increased, the production cost is reduced, and a reliable base is established for realizing the industrialized production of the ammopiptanthus mongolicus. The tissue culture method disclosed by the invention is simple and convenient to operate and low in cost and has favorable application prospects.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a method for tissue culture of holly. Background technique [0002] Ammopiptanthus mongolicus (Maxim. ex Kom.) Cheng f. is a plant of the genus Ammopiptanthus mongolicus (Maxim. ex Kom.) Cheng f., which belongs to the endangered species. It is mainly distributed in Inner Mongolia, Ningxia, Gansu, Xinjiang, southern Mongolia, central Mongolia Subregions are also distributed. Sand holly has important value in the ecological restoration of desert areas. It has excellent drought resistance, cold resistance, wind resistance and sand-fixing ability. An excellent choice for windbreak and sand fixation in the region. [0003] However, the existing seed container cultivation technology of Ilex japonica has a long seedling cycle and a low emergence rate, generally only 30-40%. Improving the survival rate of Ilex japonica seedlings through tissue culture and propagation...

AI Technical Summary

Problems solved by technology

[0003] However, the existing seed container cultivation technology of Ilex japonica has a long seedling cycle and a low emergence rate, generally only 30-40%. Improving the survival rate of Ilex japonica seedlings through tissue culture and propagation technology is an effective way to solve the problem of ecological seedlings of Ilex japonica

The tissue culture and multiplication of Ilex japonica generally includes three periods: primary induction → subgeneration → rooting culture. In the period of primary induction and subgeneration proliferation during the tissue culture of Ilex japonica, the browning phenomenon is serious, resulting in low survival rate of tissue culture seedlings. Therefore, it is very important to find the best medium formula for each period, solve the browning problem in the tissue culture process, improve the survival rate of the tissue culture seedlings, and reduce the production cost, which is extremely important for the industrialization and industrialization of the ecological seedlings of Ilex japonica