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Rapid propagation method for Ammopiptanthus mongolicus

A technology of sand holly and seeds, applied in the field of asexual reproduction, can solve the problems of perishable cuttings, separation, variation, affecting reproduction, etc., and achieve the effects of short reproduction cycle, increased rooting rate, and low cost of seedling raising.

Active Publication Date: 2018-05-29
GANSU DESERT CONTROL RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, most of Ilex japonica adopt seed sowing and propagation, and the separation and variation of hereditary characters often occur after the seedlings become seedlings; while adopting the conventional asexual propagation cutting method, Ilex japonica is difficult to take root, and the cuttings are easy to rot during the cutting process, affecting its reproduce
In the prior art, there is no systematic research report on the tissue culture cloning and rapid propagation of the high rooting rate of Ilex chinensis

Method used

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  • Rapid propagation method for Ammopiptanthus mongolicus
  • Rapid propagation method for Ammopiptanthus mongolicus
  • Rapid propagation method for Ammopiptanthus mongolicus

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preparation example Construction

[0035] In the present invention, the preparation method of sterile vaccine comprises the following steps:

[0036] a) Soaking and disinfecting the seeds of Ilex japonica, obtaining the sterilized seeds of Ilex japonica;

[0037] b) inoculate the sterilized Ilex japonica seeds in the aseptic seedling culture medium for 10-15 days to obtain aseptic seedlings; the aseptic seedling culture medium is based on the improved B5 medium, including 25g / L sucrose and 4.5g / L agar, the pH is 5.8-6.0.

[0038] The invention soaks and sterilizes the holly seeds to obtain the sterilized holly seeds. In the present invention, the holly seeds are preferably holly seeds harvested in the same year, plump and of the same size, and the holly seeds are preferably washed with water before soaking, and the washing time is preferably 4 hours. In the present invention, the flushing water is preferably flowing water. In the present invention, there is no special requirement for the soaking water, and t...

Embodiment 1

[0051] 1. The preparation of the improved B5 medium:

[0052] 1) Macroelements: potassium nitrate 2000.00mg / L, calcium chloride 100.00mg / L, magnesium sulfate 333.33mg / L, sodium dihydrogen phosphate 100.00mg / L, ammonium sulfate 89.30mg / L;

[0053] 2) Trace elements: boric acid 3.00mg / L, zinc sulfate 2.00mg / L, potassium iodide 0.75mg / L, copper sulfate 0.025mg / L, sodium molybdate 0.25mg / L, manganese sulfate 10.00mg / L, cobalt chloride 0.025 mg / L;

[0054] 3) Iron salt: diiron ethylenediaminetetraacetic acid 28.00mg / L;

[0055] 4) Organic ingredients: inositol 100.00mg / L, niacin 1.00mg / L, pyridoxine hydrochloride 1.00mg / L, thiamine hydrochloride 10.00mg / L. The above four groups of reagents were dissolved and mixed according to the group, and the volume was adjusted to 1000mL.

[0056] 2. Main steps:

[0057] 1. Seed disinfection treatment:

[0058] Select the plump and uniformly sized Ilex seeds harvested in the same year, wash them under running tap water for 4 hours, then so...

Embodiment 2

[0073] The improved B5 medium is prepared as in Example 1:

[0074] The main steps:

[0075] 1. Seed disinfection treatment:

[0076] Select the plump and uniformly sized Ilex seeds harvested in the same year, wash them under running tap water for 4 hours, then soak them in distilled water for 12 hours, then pour off the distilled water and place the fully soaked seeds on an ultra-clean workbench for disinfection.

[0077] Seed disinfection is first soaked in 20% sodium hypochlorite for 2 minutes, and the glass rod used for sterilization is constantly stirred during the soaking period to make the sterilization more thorough, and then rinsed with sterile water for 3 times; the second step is soaked in 70% ethanol for 35 seconds. During the soaking period, the glass rod used for sterilization was continuously stirred and rinsed with sterile water for 5 times; the third step was soaked with 0.1% mercuric chloride for 5 minutes, and the glass rod used for sterilization was consta...

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Abstract

The invention relates to a rapid propagation method for Ammopiptanthus mongolicus, belonging to the technical field of vegetative propagation. The rapid propagation method provided by the invention solves the problem of difficult rooting in cutting propagation of the Ammopiptanthus mongolicus on the basis of maintaining excellent characters of a valuble endangered plant namely the Ammopiptanthus mongolicus through arrangement of conditions of callus induction, differentiation culture and rooting culture for aseptic seedlings of the Ammopiptanthus mongolicus. The rapid propagation method has the advantages of short propagation period, rapid rooting rate, high survival rate and low culture cost, and can lay a good preliminary foundation for protection of Ammopiptanthus mongolicus germplasm resources and production and research of high-quality Ammopiptanthus mongolicus seedlings.

Description

technical field [0001] The invention relates to the technical field of asexual reproduction, in particular to a method for rapid propagation of holly. Background technique [0002] Ammopiptanthus mongolicu belongs to the genus Ammopiptanthus mongolicu. It is a small number of evergreen sand-growing shrubs in desert areas. It is widely distributed in Inner Mongolia, Ningxia, and Gansu. In Gansu, it is mainly distributed in the Hexi Corridor and the Heihe River Basin. Sand holly has strong adaptability to environmental conditions, drought resistance, cold resistance, barren resistance, saline and alkali resistance, and a well-developed root system, which plays a very important role in preventing sand and fixing sand, preventing soil erosion, and improving the environment. Therefore, the plant has good ecological benefits in improving the environment, and is a potential ecological tree species for vegetation restoration and reconstruction in arid and semi-arid areas to be devel...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张卫星
Owner GANSU DESERT CONTROL RES INST
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