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Propagation method of ammopiptanthus mongolicus tissue culture seedlings

A technology for holly japonica and tissue culture seedlings is applied in the field of holly japonica seedling raising, which can solve the problems of complicated operation, long seedling raising period, low seedling emergence rate and the like, and achieve the effects of simple operation, high seedling emergence rate and high reproduction coefficient.

Active Publication Date: 2018-03-23
GANSU DESERT CONTROL RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the propagation and cultivation of Ilex japonica mostly utilize the seeds of Ilex japonica to carry out container seedling cultivation, the operation is complicated, the emergence rate is low, and the seedling period is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Seed disinfection: Rinse the holly seeds with running water for 20 minutes, then disinfect them with 75% alcohol for 20 minutes; then use 0.1% HgCl 2 After the solution sterilized the seeds deeply for 10 minutes, they were washed with sterile water for 5 times, 10 minutes each time; finally, the water on the surface of the seeds was blotted with sterile filter paper.

[0032] (2) Cultivation of sterile seedlings: inoculate the sterilized seeds on the surface of 1 / 2MS solid medium, submerge the end of the seed hole into the medium for 0.3 cm, and culture at 25°C under light conditions for 10 days;

[0033] (3) Induction of axillary buds: Cut the nodal stems of aseptic seedlings of Ilex japonica with a length of 1.5 cm, and inoculate them in the axillary bud induction medium: MS+6-BA 1.5 mg / L+IBA 1 mg / L, place at 25°C, Cultivate under light conditions for 15 days; 4-6 clustered buds can be induced to grow at the stem nodes, with an average length of about 3cm, and the...

Embodiment 2

[0036] (1) Seed disinfection: Rinse the holly seeds with running water for 20 minutes, then disinfect them with 75% alcohol for 20 minutes; then use 0.1% HgCl 2 After the solution sterilized the seeds deeply for 10 minutes, they were rinsed with sterile water 6 times, 10 minutes each time; finally, the moisture on the surface of the seeds was blotted with sterile filter paper.

[0037] (2) Cultivation of sterile seedlings: inoculate the sterilized seeds on the surface of 1 / 2MS solid medium, submerge the end of the seed hole into the medium by 0.35 cm, and place them at 25°C under light conditions for 12 days;

[0038] (3) Induction of axillary buds: Cut the section of the nodal stem of the sterile seedlings of Ilex japonica with a length of 1.5cm, inoculate it in the axillary bud induction medium: MS+6-BA 1.5mg / L+IBA 1.5mg / L, place at 25°C 1. Cultivate under light conditions for 15 days; the number of clustered buds is large, the average length is about 1.5cm, and they are thi...

Embodiment 3

[0041] (1) Seed disinfection: Rinse the holly seeds with running water for 20 minutes, then disinfect them with 75% alcohol for 20 minutes; then use 0.1% HgCl 2 After the solution sterilized the seeds deeply for 10 minutes, they were rinsed with sterile water 6 times, 10 minutes each time; finally, the moisture on the surface of the seeds was blotted with sterile filter paper.

[0042] (2) Cultivation of sterile seedlings: inoculate the sterilized seeds on the surface of 1 / 2MS solid medium, submerge 0.3cm into the medium at the end of the seed hole, and cultivate for 15 days at 25°C under light conditions;

[0043] (3) Induction of axillary buds: Cut the nodal stems of aseptic seedlings of Ilex japonica with a length of 1.5 cm, and inoculate them in the axillary bud induction medium: MS+6-BA 1.5mg / L+IBA 0.5mg / L, placed at 25°C 1. Cultivate under light conditions for 15 days; only a single bud can be induced to grow, the average length is about 3cm, and the growth condition is ...

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Abstract

The invention provides a propagation method of ammopiptanthus mongolicus tissue culture seedlings. The method includes the steps: 1) sequentially flushing ammopiptanthus mongolicus seeds by the aid ofrunning water, soaking and disinfecting the flushed seeds by the aid of ethyl alcohol, soaking and disinfecting the seeds by the aid of HgCl2 solution, and flushing the seeds by the aid of sterile water to obtain disinfected seeds; 2) inoculating the disinfected seeds into 1 / 2MS solid media to culture the seeds for 10-20 days to obtain aseptic seedlings; 3) acquiring stem sections with knobs fromthe aseptic seedlings, and inoculating the stem sections into axillary bud induction media to culture the stem sections for 15-20 days to obtain axillary buds; 4) inoculating the axillary buds obtained in the steps 3) into rooting media to culture the axillary buds for 10-15 days to obtain the tissue culture seedlings. According to the method, explants are acquired from aseptic seedlings, so thatpropagation of the tissue culture seedlings is performed, the method is simple to operate, short in use time, zero in contamination rate and high in emergence rate.

Description

technical field [0001] The invention belongs to the technical field of seedling cultivation of holly holly, and in particular relates to a method for propagating tissue cultured seedlings of holly holly. Background technique [0002] Sand holly (Ammopiptanthus mongolicus) is a unique species in the desert areas of Central Asia. It is a rare and endangered protected plant in my country. It is mainly distributed in the sandy land, Gobi or rocky slopes. The origin of this species is ancient, and it is a relic of the xerophyte type of ancient Mediterranean hot and humid plants. During the long environmental evolution in Central Asia and western my country and the formation of the northwest arid climate zone, it gradually evolved into a drought-resistant, cold-resistant, and heat-resistant sandy xerophyte type. , has special morphological adaptation characteristics, physiological adaptation mechanism and molecular genetic basis of stress resistance, so it has extremely important c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张莹花尉秋实王方琳张进虎
Owner GANSU DESERT CONTROL RES INST
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