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Ammopiptanthus mongolicus low-temperature stress related gene, and expression carrier and applications thereof

A technology for low temperature stress and expression vectors, applied in the field of low temperature stress related genes and their expression vectors and applications

Active Publication Date: 2015-02-04
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no literature report on the isolation of genes related to low temperature stress from Ilex japonica

Method used

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  • Ammopiptanthus mongolicus low-temperature stress related gene, and expression carrier and applications thereof
  • Ammopiptanthus mongolicus low-temperature stress related gene, and expression carrier and applications thereof
  • Ammopiptanthus mongolicus low-temperature stress related gene, and expression carrier and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Screening of the Stress Expression Profile of Ilex chinensis

[0048] 1 Experimental materials

[0049] 1.1 Ilex japonica seeds: provided by researcher Pei Xinwu, Institute of Biotechnology, Chinese Academy of Agricultural Sciences.

[0050] 1.2 Enzyme and reagent kit

[0051] DNA polymerase Ex Taq and dNTPs were purchased from Bao Bio (Dalian) Company; RQ1RNase-Free DNase was purchased from Promega (Beijing) Biotechnology Co., Ltd. The agarose gel DNA recovery kit was purchased from Beijing Biotech Biotechnology Co., Ltd.; the reverse transcription kit was purchased from NEB Company; the first strand cDNA synthesis kit (Toyobo First Stand cDNA Synthesis Kit) was purchased from Toyobo (Shanghai) )Biological Technology Co., Ltd.

[0052] 2 Experimental methods

[0053] 2.1 Stress expression profile analysis of Ilex chinensis

[0054] The adversity transcript profile of S. hollyensis was obtained and preserved by our laboratory. About 5000 stress-related T...

Embodiment 2

[0078] Example 2 Cloning of temperature-induced expression genes of Ilex chinensis

[0079] 1 Experimental materials

[0080] 1.1 Vectors and strains: The cloning vector pEASY-T1Simple Cloning Vector and Escherichia coli (Escherichia coli) Trans1-T1Phage Resistant competent cells were purchased from Quanshijin Biotechnology Co., Ltd.

[0081] 1.2 Enzymes and kits: DNA polymerase and dNTPs were purchased from Bao Bio (Dalian) Company; RQ1RNase-Free DNase was purchased from Promega (Beijing) Biotechnology Co., Ltd. The first strand cDNA synthesis kit (Toyobo First Stand cDNA Synthesis Kit) was purchased from Toyobo (Shanghai) Biotechnology Co., Ltd.; the RACE kit was purchased from Invitrogen Life Technologies, USA; the agarose gel DNA recovery kit was purchased from Biomiga Company; EASYspin Plant RNA Rapid Extraction Kit was purchased from Yuanpinghao Biotechnology Co., Ltd.

[0082] 2 Experimental methods

[0083] 2.1 Extraction of high-purity total RNA of holly holly (kit...

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Abstract

The invention discloses an ammopiptanthus mongolicus low-temperature stress related gene, and an expression carrier and applications thereof, and belongs to the field of separation and applications of genes relating to plant stress resistance. cDNA sequences of the low-temperature stress related gene separated from ammopiptanthus mongolicus are represented by SEQ ID No.1; and coded amino acid sequences are represented by SEQ ID No.2. The invention also provides the recombined plant expression carrier containing the low-temperature stress related gene, and host cells containing the recombined plant expression carrier. In addition, the invention provides a method used for increasing or improving resistance to plant low temperature stress. The method comprises following steps: the recombined plant expression carrier containing the low-temperature gene is constructed; the recombined plant expression carrier is transformed into receptor plants or plant cells; and gene modified plant varieties are obtained via cultivating and screening. The low-temperature stress related gene separated from ammopiptanthus mongolicus possesses important application prospect in increasing plant resistance to environmental stress or cultivating low-temperature stress resistant plant varieties.

Description

technical field [0001] The invention relates to a temperature-related gene isolated from higher plants, in particular to a gene related to low temperature stress isolated from Ammopiptanthus mongolicus (Maxim.) Cheng f., and the invention further relates to a gene containing the gene related to low temperature stress The gene recombinant plant expression vectors and their application in improving the low temperature stress resistance of plants belong to the field of isolation and application of genes related to stress resistance of plants. Background technique [0002] In nature, plants are inevitably under the stress of various environmental factors. Abiotic stresses such as low temperature, high temperature, drought and high salinity can easily cause damage to plants through physiological and biochemical processes such as affecting plant cell membrane components and stomatal opening. In severe cases, it will affect the yield of crops and the planting area. Abiotic adversi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/70C12N15/84A01H5/00
Inventor 程红梅顾立江郭惠明
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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