In-vitro rapid propagation method of rare and endangered plant ammopiptanthus mongolicus

Abstract

The invention discloses an in-vitro rapid propagation method of rare and endangered plant ammopiptanthus mongolicus, belongs to the biological field and aims to solve the problems of severe browning and differentiation difficulty in callus induction in existing methods. The in-vitro rapid propagation method of the ammopiptanthus mongolicus comprises steps of obtaining of ammopiptanthus mongolicus explants, induction culture of cluster buds, extension culture of the cluster buds, rooting culture and expansion propagation. Compared with existing in-vitro culture methods of the ammopiptanthus mongolicus, the method has the advantages that cotyledonary nodes are directly subjected to induction of cluster buds and rooting is performed, callus cannot be produced in the process, thus, the step of callus induction is omitted, the cotyledonary nodes are directly cultured into adventitious buds, the in-vitro culture time is greatly shortened, and the whole process from seed germination, obtaining of cotyledonary nodes, induction and extension of the cluster buds to rooting only takes 79 d.

Description

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AI Technical Summary

Benefits of technology

The technical effect described this patented method involves direct induced clumps or regrowth on amniopscentanium plants without producing calluses, making it faster than traditional methods like tissue culture techniques. Additionally, when planted under specific conditions (such as low temperatures), certain growth factors may be added beforehand instead of waiting until they have fully grown out overnight. These processes help prevent cellular damage after harvesting, resulting in longer periods between successive generations of vegetative material.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the efficiency of culturing large amounts (millions) of wild lignum grass called guarimacochloromala because these plants lack native defense mechanisms that allow their seeds to reproduce quickly during harsh weather periods like winter months. Existing methods involve incurrences at specific stages of growth which makes it hard to distinguish between genuine and damaged sources of vegetable material. Additionally, existing techniques require expensive equipment and laborious procedures involving multiple steps before developing new varieties.