Trichoderma fungicide, preparation method thereof and application of trichoderma fungicide in improving yield and quality of beet

Abstract

The invention provides a trichoderma inoculant, a preparation method thereof and application of the trichoderma inoculant in improving yield and quality of beet, and belongs to the technical field of agricultural microbial inoculants. The trichoderma agent comprises the following components: trichoderma harzianum, trichoderma viride, purpureocillium lilacinum, bacillus amyloliquefaciens and bacillus laterosporus in a mass ratio of (3-5): (1-3): (2-4): (2-4): (1-2). According to the invention, the strains cooperate with each other to promote the growth of beet, effectively enhance the immune resistance of beet, and effectively prevent and reduce beet root rot, brown blotch and other diseases caused by continuous cropping; the photosynthesis efficiency of the beet can be enhanced, the accumulation of photosynthetic products is improved, the soil structure is improved, the number of beneficial bacteria groups in the soil is increased, and the rhizosphere environment is improved, so that the sugar content and yield of the beet are increased. By applying the microbial agent disclosed by the invention, multiple effects of increasing the yield per mu, increasing the sugar content of beet, improving soil, improving the fertilizer efficiency, resisting diseases, preventing insects and the like can be achieved, and farmers are helped to increase both production and income.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of agricultural microbial agents, and particularly relates to a Trichoderma agent, a preparation method thereof and application of the Trichoderma agent in improving yield and quality of sugar beets. BACKGROUND

[0002] Trichoderma is a genus of ascomycetes, Sordariomycetes, Hypocreales, Hypocreaceae and Trichoderma. The Trichoderma fungus regulates the composition and interaction of other microorganisms by direct antagonism and competition in the rhizosphere. In the colonization of plants, the Trichoderma fungus brings many benefits to its host as an endophyte by communicating with plants. Most Trichoderma can produce various bioactive substances, such as cell wall-degrading enzymes and secondary metabolites, which have antagonistic effects on plant pathogenic fungi, bacteria and insects, and can improve the stress resistance of crops, promote plant growth and increase the yield of agricultural products.

[0003] Sugar beet is a biennial or perennial herbaceous plant of Chenopodiaceae and Beta. The root of sugar beet is conical or fusiform, juicy, the stem is erect, the basal leaves are oblong, the upper surface is wrinkled and uneven, the lower surface has thick and convex leaf veins, and the margin is entire or slightly wavy. Sugar beet has high economic value, especially plays an important role in the sugar industry. It is the main sugar source other than sugarcane and is widely used for making sugar, feed and vegetables. Sugar beet is also rich in vitamin A and potassium and has medicinal values such as clearing heat and resolving toxins, removing blood stasis and stopping bleeding. The root of sugar beet contains rich betalain components and is often used as a food colorant. At present, in the process of sugar beet planting, excessive use of chemical fertilizers for the purpose of high yield is common, which causes the overgrowth of the aboveground part of sugar beet, the imbalance of root and leaf, and the decrease of the yield, sugar content and quality of sugar beet, thereby affecting the economic benefits of sugar beet planting farmers and sugar beet sugar-making enterprises. Long-term accumulation will also cause the destruction of soil structure and the pollution of the planting environment, and seriously restricts the sustainable and high-quality development of the sugar beet industry. SUMMARY

[0004] In view of this, the present application aims to provide a Trichoderma agent, a preparation method thereof and application of the Trichoderma agent in improving yield and quality of sugar beets, so as to solve the problem of yield and quality reduction of sugar beets caused by excessive fertilization.

[0005] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions:

[0006] The present application provides a Trichoderma agent, which comprises the following ingredients: Trichoderma harzianum, Trichoderma virens, Paecilomyces lilacinus, Bacillus amyloliquefaciens and Bacillus laterosporus.

[0007] The mass ratio of the Trichoderma harzianum, the Trichoderma virens, the Paecilomyces lilacinus, the Bacillus amyloliquefaciens and the Bacillus laterosporus is 3-5:1-3:2-4:2-4:1-2.

[0008] Preferably, the viable cell count of the Trichoderma harzianum is ≥ 1.0 x 10 10 cFu / g, the viable cell count of the Trichoderma viride is ≥ 1.0 x 10 9 cFu / g, the viable cell count of the Conidiobolus violeceus is ≥ 2.0 x 10 8 cFu / g, the viable cell count of the Bacillus amyloliquefaciens is ≥ 5.0 x 10 8 cFu / mL, the viable cell count of the Bacillus laterosporus is ≥ 2.0 x 10 10 cFu / g.

[0009] The application also provides a preparation method of the Trichoderma agent, comprising the following steps:

[0010] 1) Trichoderma harzianum, Trichoderma viride and Conidiobolus violeceus are inoculated into PDA culture medium respectively for culture to obtain Trichoderma harzianum suspension, Trichoderma viride suspension and Conidiobolus violeceus suspension; Bacillus amyloliquefaciens is inoculated into LB culture medium for culture to obtain Bacillus amyloliquefaciens suspension; and Bacillus laterosporus is inoculated into NA culture medium for culture to obtain Bacillus laterosporus suspension;

[0011] 2) The bacterial suspensions in step 1) are inoculated into a fermentation tank respectively for fermentation culture, and then dried under reduced pressure and crushed to obtain corresponding bacterial powder;

[0012] 3) The Trichoderma harzianum, Trichoderma viride, Conidiobolus violeceus, Bacillus amyloliquefaciens and Bacillus laterosporus bacterial powder obtained in step 2) are uniformly mixed to obtain the Trichoderma agent.

[0013] Preferably, the culture temperature of the Trichoderma harzianum in step 1) is 25-35℃, and the culture time is 5-7d; the culture temperature of the Trichoderma viride is 25-28℃, and the culture time is 5-7d; and the culture temperature of the Conidiobolus violeceus is 25-30℃, and the culture time is 5-7d.

[0014] Preferably, the culture temperature of the Bacillus amyloliquefaciens in step 1) is 31-37℃, and the culture time is 16-24h; and the culture temperature of the Bacillus laterosporus is 28-37℃, and the culture time is 18-24h.

[0015] Preferably, the culture temperature of the fermentation tank in step (2) is 28-30℃, and the fermentation culture time is 6-8d.

[0016] The application also provides application of the Trichoderma agent in improving the yield and quality of sugar beets.

[0017] Compared with the prior art, the application has the following beneficial effects:

[0018] The Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus in the application can promote the growth of sugar beets, effectively enhance the immune resistance of sugar beets, effectively prevent and reduce sugar beet root rot, brown spot and other diseases caused by continuous cropping, enhance the photosynthesis efficiency of sugar beets, improve the accumulation of photosynthetic products, improve the soil structure, increase the number of soil beneficial bacteria, and improve the rhizosphere environment, thereby increasing the sugar content and yield of sugar beets. The application of the microbial agent can achieve the multiple effects of increasing yield per mu, increasing sugar content, improving soil, improving fertilizer efficiency, resisting diseases and pests, and the like, thereby helping farmers to increase yield and income. DETAILED DESCRIPTION

[0019] The application provides a Trichoderma agent, which comprises the following components: Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus; the mass ratio of the Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus is preferably 3-5:1-3:2-4:2-4:1-2, and is further preferably 3.5-4.5:1.5-2.5:2.5-3.5:2.5-3.5:1.2-1.8.

[0020] In the application, the viable count of the Trichoderma harzianum is ≥1.0×10 10 cFu / g, the viable count of the Trichoderma viride is ≥1.0×10 9 cFu / g, the Trichoderma harzianum and the Trichoderma viride can produce antagonistic effects on various plant pathogenic fungi through mechanisms such as nutritional competition, hyperparasitism, cell wall-decomposing enzymes and induction of resistance in crops, thereby effectively preventing and treating soil-borne fungal diseases. The dual effects of protection and treatment enable sugar beets to reduce the occurrence of diseases, thereby improving the yield and sugar content of sugar beets. Secondly, the metabolic products of the Trichoderma harzianum and the Trichoderma viride can inhibit the proliferation of pathogenic microorganisms, thereby further reducing the occurrence of sugar beet diseases. These metabolic products can inhibit the growth of pathogenic fungi by secreting special substances, thereby protecting the healthy growth of sugar beets. In addition, the Trichoderma harzianum and the Trichoderma viride can produce natural plant growth regulators after being activated in the soil, thereby promoting the growth and downward penetration of sugar beet roots and increasing the nutrient absorption rate of sugar beets. The growth regulation effect not only enhances the disease resistance of sugar beets, but also improves the yield and quality of sugar beets.

[0021] In the application, the viable count of the Purpureocillium lilacinum is ≥2.0×10 8The mycelium of the L. lilacina can penetrate the egg shell, the larva and the female adult body wall of the plant parasitic nematode, absorbs nutrients in the body, reproduces, destroys the normal physiological metabolism of the egg, the larva and the female adult, and causes the death of the plant parasitic nematode.

[0022] In the present application, the viable cell count of the Bacillus amyloliquefaciens is ≥5.0×10 8 The Bacillus amyloliquefaciens can induce the beet to produce superoxide dismutase (SOD), polyphenol oxidase (PPO), peroxidase (POD) and the like, improve the stress resistance of the beet, induce the plant to rapidly secrete endogenous auxin, promote the beet to rapidly root, improve the root development ability, and promote the healthy growth of the plant.

[0023] In the present application, the viable cell count of the Bacillus laterosporus is ≥2.0×10 10 The Bacillus laterosporus can secrete various extracellular enzymes such as chitinase, protease, amylase, carbonase, hemicellulase, lipase and the like as well as natural plant hormones, promote the absorption of nutrients by the beet, decompose the rich organic matter in the soil into nutrient substances that can be utilized by the beet, stimulate the growth and root development of the beet, and thus increase the yield and income.

[0024] The present application further provides a preparation method of the Trichoderma agent, comprising the following steps:

[0025] 1) inoculate the Trichoderma harzianum, the Trichoderma viride and the L. lilacina into PDA culture medium respectively to obtain Trichoderma harzianum suspension, Trichoderma viride suspension and L. lilacina suspension; inoculate the Bacillus amyloliquefaciens into LB culture medium to obtain Bacillus amyloliquefaciens suspension; and inoculate the Bacillus laterosporus into NA culture medium to obtain Bacillus laterosporus suspension;

[0026] 2) inoculate the suspensions in step 1) into fermentation tanks respectively to carry out fermentation culture, and then carry out drying under reduced pressure and crushing to obtain corresponding bacterial powder;

[0027] 3) mixing the Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus powders obtained in step 2) to obtain the Trichoderma agent.

[0028] In the present application, the culture temperature of the Trichoderma harzianum in step 1) is preferably 25-35℃, further preferably 27-33℃, and the culture time is preferably 5-7d, further preferably 5.5-6.5d; the culture temperature of the Trichoderma viride is preferably 25-28℃, further preferably 26-27℃, and the culture time is preferably 5-7d, further preferably 5.5-6.5d; the culture temperature of the Purpureocillium lilacinum is preferably 25-30℃, further preferably 26-29℃, and the culture time is preferably 5-7d, further preferably 5.5-6.5d; the culture temperature of the Bacillus amyloliquefaciens is preferably 31-37℃, further preferably 32-35℃, and the culture time is preferably 16-24h, further preferably 18-22h; and the culture temperature of the Bacillus laterosporus is preferably 28-37℃, further preferably 29-36℃, and the culture time is preferably 18-24h, further preferably 19-23h.

[0029] In the present application, the culture temperature of the fermenter in step (2) is preferably 28-30℃, further preferably 29℃, and the culture time is preferably 6-8d, further preferably 7d.

[0030] The present application also provides the use of the Trichoderma agent in improving the yield and quality of sugar beets.

[0031] The technical solutions provided by the present application will be described in detail below in conjunction with the examples, but they should not be understood as limiting the scope of protection of the present application.

[0032] The strains used in the present application are as follows: Trichoderma harzianum ACCC 30371, Purpureocillium lilacinum purchased from Beijing Bao Wei Biotechnology Co., Ltd.;

[0033] Trichoderma viride CICC 40903 purchased from Beijing Yubi Technology Co., Ltd.;

[0034] Bacillus amyloliquefaciens ACCC 60428, Bacillus subtilis ACCC 62258, and Trichoderma asperellum ACCC 30536 purchased from China Agricultural Microbial Culture Collection Center;

[0035] Bacillus laterosporus CICC 21185 purchased from China Industrial Microbial Culture Collection Center.

[0036] Example 1

[0037] A Trichoderma agent, which is composed of Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus; the mass ratio of the Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus is 4:2:3:3:1.5.

[0038] The preparation method of the Trichoderma agent is as follows: the Trichoderma harzianum is inoculated into PDA medium and cultured at a temperature of 30 DEG C for 6 days to obtain a Trichoderma harzianum suspension, the Trichoderma viride is inoculated into PDA medium and cultured at a temperature of 26 DEG C for 6 days to obtain a Trichoderma viride suspension, the Purpureocillium lilacinum is inoculated into PDA medium and cultured at a temperature of 28 DEG C for 6 days to obtain a Purpureocillium lilacinum suspension; the Bacillus amyloliquefaciens is inoculated into LB medium and cultured at a temperature of 34 DEG C for 20 hours to obtain a Bacillus amyloliquefaciens suspension; the Bacillus laterosporus is inoculated into NA medium and cultured at a temperature of 30 DEG C for 20 hours to obtain a Bacillus laterosporus suspension; the Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus suspensions are respectively inoculated into a fermenter and fermented at a temperature of 29 DEG C for 7 days, and then dried under reduced pressure and crushed to obtain corresponding bacterial powders; the obtained Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus bacterial powders are uniformly mixed to obtain the Trichoderma agent. The viable bacterial count of the Trichoderma harzianum in the Trichoderma agent is ≥1.0x10 10 CFU / g, the viable bacterial count of the Trichoderma viride is ≥1.0x10 9 CFU / g, the viable bacterial count of the Purpureocillium lilacinum is ≥2.0x10 8 CFU / g, the viable bacterial count of the Bacillus amyloliquefaciens is ≥5.0x10 8 CFU / mL, and the viable bacterial count of the Bacillus laterosporus is ≥2.0x10 10 CFU / g.

[0039] Example 2

[0040] A Trichoderma agent, which is composed of Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus; the mass ratio of the Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus is 4:2:3:3:1.5.

[0041] The preparation method of the Trichoderma agent is as follows: Trichoderma harzianum is inoculated into PDA medium and cultured at a temperature of 25 DEG C for 5 days to obtain a Trichoderma harzianum suspension, Trichoderma viride is inoculated into PDA medium and cultured at a temperature of 25 DEG C for 5 days to obtain a Trichoderma viride suspension, Byssochlamys nivea is inoculated into PDA medium and cultured at a temperature of 25 DEG C for 5 days to obtain a Byssochlamys nivea suspension, Bacillus amyloliquefaciens is inoculated into LB medium and cultured at a temperature of 31 DEG C for 16 hours to obtain a Bacillus amyloliquefaciens suspension, Bacillus laterosporus is inoculated into NA medium and cultured at a temperature of 28 DEG C for 18 hours to obtain a Bacillus laterosporus suspension; the Trichoderma harzianum suspension, the Trichoderma viride suspension, the Byssochlamys nivea suspension, the Bacillus amyloliquefaciens suspension and the Bacillus laterosporus suspension are respectively inoculated into a fermenter and fermented at a temperature of 28 DEG C for 6 days, and then dried under reduced pressure and crushed to obtain corresponding bacterial powder; the obtained Trichoderma harzianum bacterial powder, the Trichoderma viride bacterial powder, the Byssochlamys nivea bacterial powder, the Bacillus amyloliquefaciens bacterial powder and the Bacillus laterosporus bacterial powder are uniformly mixed to obtain the Trichoderma agent. 10 The viable bacterial count of the Trichoderma harzianum in the Trichoderma agent is ≥1.0x10 9 The viable bacterial count of the Trichoderma viride in the Trichoderma agent is ≥1.0x10 8 The viable bacterial count of the Byssochlamys nivea in the Trichoderma agent is ≥2.0x10 8 The viable bacterial count of the Bacillus amyloliquefaciens in the Trichoderma agent is ≥5.0x10 10 The viable bacterial count of the Bacillus laterosporus in the Trichoderma agent is ≥2.0x10

[0042] Example 3

[0043] A Trichoderma agent, which is composed of the following components: Trichoderma harzianum, Trichoderma viride, Byssochlamys nivea, Bacillus amyloliquefaciens and Bacillus laterosporus; the mass ratio of the Trichoderma harzianum, the Trichoderma viride, the Byssochlamys nivea, the Bacillus amyloliquefaciens and the Bacillus laterosporus is 5:3:4:4:2.

[0044] The preparation method of the Trichoderma agent is as follows: the Trichoderma harzianum is inoculated into PDA culture medium and cultured at a temperature of 35℃ for 7 days to obtain a Trichoderma harzianum suspension, the Trichoderma viride is inoculated into PDA culture medium and cultured at a temperature of 28℃ for 7 days to obtain a Trichoderma viride suspension, the B. purpurea is inoculated into PDA culture medium and cultured at a temperature of 30℃ for 7 days to obtain a B. purpurea suspension, the B. amyloliquefaciens is inoculated into LB culture medium and cultured at a temperature of 37℃ for 24 hours to obtain a B. amyloliquefaciens suspension, the B. laterosporus is inoculated into NA culture medium and cultured at a temperature of 37℃ for 24 hours to obtain a B. laterosporus suspension, the Trichoderma harzianum, the Trichoderma viride, the B. purpurea, the B. amyloliquefaciens and the B. laterosporus suspensions are respectively inoculated into a fermentation tank and fermented at a temperature of 30℃ for 8 days, and then dried under reduced pressure and crushed to obtain corresponding bacterial powders; and the obtained Trichoderma harzianum, Trichoderma viride, B. purpurea, B. amyloliquefaciens and B. laterosporus bacterial powders are uniformly mixed to obtain the Trichoderma agent. In the Trichoderma agent, the viable count of the Trichoderma harzianum is ≥1.0×10 10 CFU / g, the viable count of the Trichoderma viride is ≥1.0×10 9 CFU / g, the viable count of the B. purpurea is ≥2.0×10 8 CFU / g, the viable count of the B. amyloliquefaciens is ≥5.0×10 8 CFU / mL, and the viable count of the B. laterosporus is ≥2.0×10 10 CFU / g.

[0045] Comparative Example 1

[0046] The Trichoderma harzianum in Example 1 is replaced by Trichoderma asperellum.

[0047] Comparative Example 2

[0048] The B. amyloliquefaciens in Example 1 is replaced by B. subtilis.

[0049] Comparative Example 3

[0050] The mass ratio of the Trichoderma harzianum, the Trichoderma viride, the B. purpurea, the B. amyloliquefaciens and the B. laterosporus in Example 1 is changed to “1:1:1:1:1”.

[0051] Comparative Example 4

[0052] The mass ratio of the Trichoderma harzianum, the Trichoderma viride, the B. purpurea, the B. amyloliquefaciens and the B. laterosporus in Example 1 is changed to “6:4:1:1:3”.

[0053] Experimental Example 1

[0054] Sugar beet variety: IM1162

[0055] A randomized block design is adopted, with no fungicide application as the control (CK), and the fungicide treatments of Examples 1-3 and Comparative Examples 1-4 are set, each treatment is repeated 3 times, a total of 30 plots, the plot length is 6 m, the plot width is 5 m, the application amount of the fungicide is 5 kg / mu, and other conditions such as field management are the same. The yield, sugar content rate, sugar yield and root rot incidence of sugar beets in different treatment groups are counted.

[0056] 1. Yield determination: 4 rows are harvested for yield determination in each treatment and each repetition, and the yield per hectare is converted, wherein the root cutting method is carried out according to GB / T 10496-2018.

[0057] 2. Sugar content rate (%): 15 sugar beets are selected from each yield determination plot, a sugar tester is used to determine the degree of hammering, and then the sugar content rate is converted and the average value is calculated.

[0058] Sugar yield (kg / hm 2 ) = yield (kg / hm 2 ) x sugar content rate (%)

[0059] 3. At the harvesting period of sugar beets, 50 plants are randomly sampled from the whole test area in each plot, and the incidence of sugar beet root rot is investigated according to the unified standard. Incidence rate = number of diseased plants / total number of plants investigated x 100%.

[0060] Experimental results: as shown in Table 1.

[0061] Table 1: Yield, sugar content rate, sugar yield and root rot incidence of sugar beets in different treatment groups

[0062]

[0063]

[0064] As shown in Table 1, the yield, sugar content rate and sugar yield of sugar beets treated by Examples 1-3 are higher than those of CK and Comparative Examples 1-4, and the root rot incidence is lower than that of CK and Comparative Examples 1-4. The types and dosage ratios of the strains in the fungicide significantly affect the yield and quality of sugar beets. The fungicide of the present application not only has a certain inhibitory effect on sugar beet root rot, but also can greatly improve the yield and sugar content rate of sugar beets.

[0065] As can be seen from the above examples and experimental examples, the Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus in the present application can not only promote the growth of sugar beets, but also effectively enhance the immune resistance of sugar beets, prevent and reduce sugar beet root rot, brown spot and other diseases caused by heavy cropping; can also enhance the photosynthetic efficiency of sugar beets, improve the accumulation of photosynthetic products, improve the soil structure, increase the number of beneficial bacteria in the soil, and improve the rhizosphere environment, thereby increasing the sugar content and yield of crops.

[0066] The above merely describes the preferred embodiments of the present application, and it should be pointed out that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered as falling within the protection scope of the present application.

Claims

1. A Trichoderma agent, characterized in that, The composition comprises the following ingredients: Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus. The mass ratio of the Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus is 3-5:1-3:2-4:2-4:1-2.

2. The Trichoderma agent according to claim 1, characterized in that, the number of viable cells of said Trichoderma harzianum is ≥ 1.0 x 10 10 cFu / g, the number of viable cells of said Trichoderma viride is ≥ 1.0 x 10 9 cFu / g, the number of viable cells of said Conidiobolus vagans is ≥ 2.0 x 10 8 cFu / g, the number of viable cells of said Bacillus amyloliquefaciens is ≥ 5.0 x 10 8 cFu / g, the number of viable cells of said Bacillus laterosporus is ≥ 2.0 x 10 10 cFu / g.

3. The method of producing a Trichoderma agent according to claim 1 or 2, characterized in that, The method comprises the following steps: 1) Trichoderma harzianum, Trichoderma viride and Purpureocillium lilacinum are respectively inoculated into PDA medium for culture to obtain Trichoderma harzianum suspension, Trichoderma viride suspension and Purpureocillium lilacinum suspension; Bacillus amyloliquefaciens is inoculated into LB medium for culture to obtain Bacillus amyloliquefaciens suspension; and Bacillus laterosporus is inoculated into NA medium for culture to obtain Bacillus laterosporus suspension; 2) The bacterial suspensions in step 1) are respectively inoculated into a fermentation tank for fermentation culture, and then dried under reduced pressure and crushed to obtain corresponding bacterial powder; 3) The Trichoderma harzianum, Trichoderma viride, Purpureocillium lilacinum, Bacillus amyloliquefaciens and Bacillus laterosporus bacterial powder obtained in step 2) are uniformly mixed to obtain the Trichoderma agent.

4. The preparation method according to claim 3, characterized in that, The culture temperature of the Trichoderma harzianum in step 1) is 25-35℃, and the culture time is 5-7d; the culture temperature of the Trichoderma viride is 25-28℃, and the culture time is 5-7d; and the culture temperature of the Purpureocillium lilacinum is 25-30℃, and the culture time is 5-7d.

5. The preparation method according to claim 3, characterized in that, The culture temperature of the Bacillus amyloliquefaciens in step 1) is 31-37℃, and the culture time is 16-24h; and the culture temperature of the Bacillus laterosporus is 28-37℃, and the culture time is 18-24h.

6. The preparation method according to claim 3, characterized in that, The culture temperature of the fermentation tank in step (2) is 28-30℃, and the fermentation culture time is 6-8d.

7. The Trichoderma agent of claim 1 or 2 for use in improving the yield and quality of sugar beets.

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