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A method for transplanting rootless tissue-cultured seedlings of delicious kiwifruit rootstock

A technology of kiwifruit and tissue culture seedlings, which is applied in the field of transplanting rootless tissue culture seedlings of delicious kiwifruit rootstocks, can solve the problems of increasing the time of kiwifruit tissue culture propagation, the cost of seedling cultivation, time-consuming and labor-intensive, etc., to shorten the propagation time and Seedling cost, simplified seedling technology, and the effect of solving browning

Active Publication Date: 2021-08-27
WUHAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the existing kiwifruit tissue culture seedlings need to be rooted and cultivated, and can be transplanted after hardening and domestication. However, rooting culture and hardening are time-consuming and labor-intensive, which greatly increases the time and cost of kiwifruit tissue culture and breeding.

Method used

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  • A method for transplanting rootless tissue-cultured seedlings of delicious kiwifruit rootstock
  • A method for transplanting rootless tissue-cultured seedlings of delicious kiwifruit rootstock
  • A method for transplanting rootless tissue-cultured seedlings of delicious kiwifruit rootstock

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 explant processing

[0032]Rinse the branches collected in the field for 30 minutes under running water, place them on the ultra-clean workbench, sterilize them with 75% alcohol for 3 minutes, rinse them with sterile water for 4 times, and use 0.1% HgCl 2 The solution was sterilized, the time was set to 4, 6, 8, 10, 12 min, and the sterile water was washed 4 times. The sterilized stem section is cut into 1.5cm length, and each stem section has an axillary bud, which is inserted vertically in the medium, and the medium is MS medium with 0.2mg / LNAA and 2mg / L6-BA added. After 30 days, the pollution rate was counted. At the same time, explore the alcohol disinfection test after raising mercury first, and the time setting remains unchanged. The test results are shown in Table 1.

[0033] Rinse the branches collected in the field under running water for 30 minutes, add 0.1ml / L bristol clear to soak for 10 minutes (with no bristol clear as the control), rinse 3...

Embodiment 2

[0040] Embodiment 2 adventitious bud regeneration induction

[0041] The terminal buds or axillary buds of the above-mentioned sterilized twigs were cut in the ultra-clean workbench as explants. When cutting the terminal buds, try to cut off all the small leaves and fluff around them, and keep about 0.3-0.5cm and insert them vertically into the bud induction medium; About 1cm, insert the bud induction medium, so that the axillary buds face up. The bud induction medium is MS medium supplemented with 0.2mg / L NAA and 1-5mg / L6-BA. 1 stem section in each culture bottle, each treatment 10 bottles, repeated 3 times. They were cultured under the conditions of light intensity 3000-3200Lux, light duration 12h / d, and temperature 24°C. Count the survival rate after 30 days, and investigate whether there are phenomena such as browning and vitrification. The test results are shown in Table 3.

[0042] Table 3 The effect of different mass volume ratios of 6-BA on the induction of advent...

Embodiment 3

[0046] Embodiment 3 Adventitious bud proliferation culture

[0047] With MS as basic medium, 6-BA and GA3 were added for treatment. The concentration gradient of 6-BA was set to 0.3mg / L, 0.5mg / L, and 0.7mg / L. The treatment of GA3 was divided into two types: adding before sterilization and adding after sterilization. The concentration gradient before sterilization was set at 1mg / L. L, 2mg / L, 3mg / L, 4mg / L, 5mg / L, the concentration gradient after sterilization is set to 0.1mg / L, 0.2mg / L, 0.3mg / L, 0.4mg / L (after filtering through the filter, add ). 3-4 stem segments per bottle, 10 bottles per treatment, repeated 3 times. Placed under the conditions of light intensity 3000-3200Lux, light 12h / d, and temperature 24°C, the proliferation of adventitious buds was cultivated. After 30 days, the proliferation coefficient was counted to investigate whether there were browning and vitrification. The test results are shown in Table 4.

[0048] Table 4 Effects of different mass-volume rat...

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Abstract

The invention provides a method for transplanting tissue-cultured seedlings of delicious kiwifruit stock, and belongs to the technical field of plant tissue-culture propagation. In this method, the tissue-cultured clustered shoots of the delicious kiwifruit 'Jinkui' after multiplication and cultivation are rinsed, cut and separated into individual seedlings, and then sterilized, and then the lower end of the unrooted individual seedlings is evenly covered with rooted and live roots Powder, directly inserted into the seedling tray filled with the substrate, carried out normal seedling cultivation, and transplanted to the nursery after two months. The invention adopts the rooting agent treatment instead of the rooting culture and seedling hardening treatment, and through the control and management in the transplanting process, a higher transplanting survival rate is achieved, and at the same time, the kiwifruit tissue culture propagation time and seedling raising cost are greatly shortened.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and propagation, relates to the tissue culture rapid propagation technology of kiwifruit, in particular to a method for transplanting rootless tissue culture seedlings of delicious kiwifruit stock. Background technique [0002] Actinidia is a perennial deciduous vine of the genus Actinidia of the family Actinidiaceae. The taxonomy of Actinidia plants is relatively difficult, and the traits of different varieties often vary and overlap. In 1911, Dunn revised for the first time that there were 23 species of Actinidia. As time went by, scientists gradually discovered more and more species. In 2002 Cui et al. identified 66 species of Actinidia genus, 62 of which are in China (XinweiLi, Jianqiang Li..Advances in the study of the systematics of ActinidiaLindley[J]Frontiers of Biology in China, 2009, (1):55-61). Common kiwifruits include Chinese kiwifruit, delicious kiwifruit, soft-date kiw...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 戢小梅乐有章刘亮陈志伟杜迎军张鸿赵志远翟敬华李长林
Owner WUHAN ACADEMY OF AGRI SCI
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