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A method for inducing plant regeneration and constructing a transgenic plant regeneration system in each part of Eucommia ulmoides

A technology for inducing plants and constructing methods, which is applied in the fields of inducing plant regeneration in various parts of Eucommia ulmoides and constructing a transgenic plant regeneration system, which can solve the problems of low rooting rate and germination rate, achieve high transformation rate and survival rate, simple operation, The effect of a wide range of applications

Active Publication Date: 2022-07-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The plant tissue culture technology of cutting and grafting has disadvantages such as low rooting rate and germination rate, so the plant tissue culture technology of Eucommia still needs to be perfected

Method used

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  • A method for inducing plant regeneration and constructing a transgenic plant regeneration system in each part of Eucommia ulmoides
  • A method for inducing plant regeneration and constructing a transgenic plant regeneration system in each part of Eucommia ulmoides
  • A method for inducing plant regeneration and constructing a transgenic plant regeneration system in each part of Eucommia ulmoides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Take the mature and full Eucommia seeds that year, cut the shell of the Eucommia seeds in the middle, first cut off the tips of both ends of the seed embryo, and then draw a wound in the middle of the remaining part for use. Inoculated in seed embryo callus induction medium: basal medium+4.5mg / L 2,4-D+0.4mg / L ZT (added after sterilization)+480mg / L proline+480mg / L glutamine Amide+15g / L glucose+30g / L sucrose+8g / L agar, pH=5.8, culture temperature is 25℃, light intensity is 1800lx, daily light time is 16h, callus state is counted after 30 days, callus induction rate= The number of callus seedlings induced / total seedlings × 100%. The components of the basal medium are: potassium nitrate 1900mg / L, ammonium nitrate 1650mg / L, potassium dihydrogen phosphate 170mg / L, magnesium sulfate 370mg / L, calcium chloride 440mg / L, potassium iodide 0.83mg / L, boric acid 6.2 mg / L, manganese sulfate 22.3mg / L, zinc sulfate 8.6mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chl...

Embodiment 2

[0044] The difference from Example 1 is that the seed embryo callus induction medium is: basal medium+5mg / L 2,4-D+0.5mg / L ZT (added after sterilization)+500mg / L proline +500mg / L glutamine+15g / L glucose+30g / L sucrose+8g / L agar, pH=5.8, the rest are the same as in Example 1.

Embodiment 3

[0046] The difference from Example 1 is that the seed embryo callus induction medium is: basal medium+5.5mg / L 2,4-D+0.6mg / L ZT (added after sterilization)+520mg / L proline Acid+520mg / L glutamine+15g / L glucose+30g / L sucrose+8g / L agar, pH=5.8, the rest are the same as in Example 1.

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Abstract

The invention provides a method for inducing plant regeneration and constructing a transgenic plant regeneration system for the whole plant of each part of Eucommia ulmoides, belonging to the technical field of woody plant tissue culture. The method for inducing plant regeneration by using the whole plant of each part of Eucommia ulmoides provided by the present invention includes the following steps: obtaining callus by induction of Eucommia seed embryos, stems and / or roots, and obtaining complete regeneration of Eucommia ulmoides through differentiated culture and rooting culture of the callus Plant; the medium of the differentiation culture is: basal medium+0.5-2mg / L6-BA+1.0-2.0mg / LIAA+25-35g / L sucrose+8-10g / L agar, pH is 5.8-6. The method for plant regeneration provided by the invention can be carried out through the roots, stems and seed embryos of Eucommia ulmoides, which has a wider application range, and the corresponding parts can be selected for regeneration according to needs, and the obtained tissue culture regeneration seedlings have corresponding Excellent traits, the cultivated tissue culture seedlings have a consistent and good growth state, are not easy to contaminate bacteria, are not easy to brown, and have a high differentiation rate and rooting rate.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of woody plants, and in particular relates to a method for inducing plant regeneration of all parts of Eucommia ulmoides and constructing a transgenic plant regeneration system. Background technique [0002] Eucommia ulmoides (scientific name Eucommia ulmoides Oliver), also known as bakelite, is a plant of the Eucommia family Eucommia genus. The tree height can reach 20 meters and the diameter at breast height is about 50 cm. It is a wild medicinal and economic plant resource in my country. my country's annual demand for natural rubber is extremely high, and the annual consumption of natural rubber ranks first in the world. my country's natural rubber mainly uses the clover rubber of Hainan as the raw material. Hevea is a tropical plant, and there are very few areas suitable for its growth in my country. It can only be cultivated in Hainan, Xishuangbanna and other areas, and the output has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H4/00
CPCA01H4/008A01H4/001
Inventor 杨清孟冬杨琬珑付玉杰杜婷婷刘腾跃董碧莹宋治华祁萌
Owner BEIJING FORESTRY UNIVERSITY
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