Induction and extraction separation method of marine organism-sourced high-efficiency heavy metal removing agent shellfish metallothionein

A technology of metallothionein and marine organisms, which is applied in the field of high-value development of aquatic products, can solve problems such as cumbersome production processes, pollute the environment, and expensive MT, so as to reduce the removal process, reduce the impact of safety, and reduce industrial cost effect

Active Publication Date: 2015-09-16
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most common MTs in the domestic market are purified from rabbit liver. Due to the special demand for MT raw materials, and the extraction technology of rabbit liver MT usually adopts buffer extraction, organic solvent precipitation to remove impurities, heat denaturation to remove impurities, and ethanol precipitation MT. and other processes, due to the use of a large amount of ethanol and chloroform in the process, not only the production process is cumbersome, but there is no effective method to produce MT in large quantities, which makes the price of MT extremely expensive and pollutes the environment, thereby limiting its application and research. MT raw materials Sources have become one of the bottlenecks restricting the development of this field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Select bivalve oysters, use seawater to wash the attached crops on the surface of the shellfish, and then temporarily raise them in clean seawater for 7 days, maintain continuous oxygenation, and remove dead shellfish in time during the temporary cultivation. After the bivalve molluscs are temporarily raised, they are cultured in seawater containing 40 mg / L zinc salt for efficient directional induction. During this period, natural light is maintained, air is continuously inflated, and seawater with metal ion solution is replaced according to the water quality. The induction time is 20 days. The seawater temperature was maintained at 20°C, the induced seawater salinity was maintained at 28‰, and the induced seawater pH was controlled at 8.2. The bivalve molluscs are induced by metal orientation, and the shells are opened to take the meat. After homogenization, weigh 50Kg of the homogenized raw material, and put in 200L 0.25 mol / L Tris-HCL buffer solution according to the ...

Embodiment 2

[0039] Select bivalve scallops, use seawater to wash the attached crops on the surface of the shellfish, and then temporarily raise them in clean seawater for 5 days, maintain continuous oxygenation, and remove dead shellfish in time during the temporary cultivation. After the bivalve molluscs are temporarily raised, they are cultured in seawater containing 0.5mg / L cadmium salt for high-efficiency directional induction. During this period, natural light is maintained, air is continuously inflated, and the seawater with metal ion solution is replaced according to the water quality. The induction time is 10 days. The seawater temperature was maintained at 18°C, the induced seawater salinity was maintained at 26‰, and the induced seawater pH was controlled at 8.2. After the bivalve molluscs are induced by metal orientation, the shells are opened to take the meat, and after homogenization, weigh 50Kg of the homogenized raw material, and put in 500L 0.05 mol / L sodium chloride buffer...

Embodiment 3

[0041] Select bivalve clams, use seawater to wash the crops attached to the surface of the shellfish, and then temporarily raise them in clean seawater for 3 days, maintain continuous oxygenation, and remove dead shellfish in time during the temporary cultivation. After the bivalve molluscs are temporarily raised, they are cultured in seawater containing 0.3mg / L copper salt for efficient directional induction. During this period, natural light is maintained, air is continuously inflated, and the seawater with metal ion solution is replaced according to the water quality. The induction time is 30 days. The seawater temperature was maintained at 22°C, the induced seawater salinity was maintained at 30‰, and the induced seawater pH was controlled at 8.5. The bivalve molluscs were induced by metal orientation, and the shells were opened to take the meat. After homogenization, 50Kg of homogenized raw materials were weighed, and 250L of 0.15 mol / L phosphate buffer solution was put in...

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Abstract

The invention discloses an induction and extraction separation method of marine organism-sourced high-efficiency heavy metal removing agent shellfish metallothionein. The induction and extraction separation method comprises following steps: (1) a selected bivalve mollusk is subjected to 2 to 7 days of temporary rearing in clean seawater, and dead ones are removed; (2) directional induction is carried out; (3) shells are open so as to collect shellfish meat, a buffer solution is added at a certain ratio, and an obtained extracted solution is subjected to thermal treatment so as to remove impurity protein; (4) the extracted solution is cooled, and is delivered through a screen so as to remove precipitates via filtering, an obtained supernate is subjected to multi-stage ultrafiltration and microfiltration, is delivered through a nanofiltration multielement film integrated purification system so as to remove macromolecules, and is subjected separation purification and concentration so as to obtain a MT crude product; and (5) the MT crude product is subjected to dextrangel and ion-exchange column chromatography combined-purification separation, and drying so as to obtain a high-purity marine organism-sourced MT product. The induction and extraction separation method is capable of increasing metallothionein content in shellfish effectively, and obtaining marine organism-sourced high-efficiency heavy metal antidote products which possess high efficiency removing effects on heavy metal, and are safer.

Description

technical field [0001] The invention relates to the technical field of high-value development of aquatic products, in particular to marine bivalve shellfish metallothionein, which is induced by metals and extracted and separated to efficiently remove heavy metals, using marine bivalve molluscs as raw materials. Background technique [0002] With the process of industrialization in our country, a large amount of industrial and agricultural wastewater has been discharged into natural water bodies, causing serious pollution in coastal waters, among which heavy metal ions have seriously polluted water bodies. When the environment is polluted by heavy metals, it can not only affect the physiological functions of marine organisms such as feeding, growth and reproduction, but also accumulate in the organisms and enter the human body through the food chain to pose a potential threat, causing acute and chronic poisoning, such as "Minamata disease" ", "Osteoarthritis", "leukopenia" an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/825C07K1/36C07K1/34C07K1/18C07K1/16
CPCC07K14/825
Inventor 孙继鹏阎光宇李诗逸易瑞灶杨婷
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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