Induction and extraction separation method of marine organism-sourced high-efficiency heavy metal removing agent shellfish metallothionein

Abstract

The invention discloses an induction and extraction separation method of marine organism-sourced high-efficiency heavy metal removing agent shellfish metallothionein. The induction and extraction separation method comprises following steps: (1) a selected bivalve mollusk is subjected to 2 to 7 days of temporary rearing in clean seawater, and dead ones are removed; (2) directional induction is carried out; (3) shells are open so as to collect shellfish meat, a buffer solution is added at a certain ratio, and an obtained extracted solution is subjected to thermal treatment so as to remove impurity protein; (4) the extracted solution is cooled, and is delivered through a screen so as to remove precipitates via filtering, an obtained supernate is subjected to multi-stage ultrafiltration and microfiltration, is delivered through a nanofiltration multielement film integrated purification system so as to remove macromolecules, and is subjected separation purification and concentration so as to obtain a MT crude product; and (5) the MT crude product is subjected to dextrangel and ion-exchange column chromatography combined-purification separation, and drying so as to obtain a high-purity marine organism-sourced MT product. The induction and extraction separation method is capable of increasing metallothionein content in shellfish effectively, and obtaining marine organism-sourced high-efficiency heavy metal antidote products which possess high efficiency removing effects on heavy metal, and are safer.

Description

technical field [0001] The invention relates to the technical field of high-value development of aquatic products, in particular to marine bivalve shellfish metallothionein, which is induced by metals and extracted and separated to efficiently remove heavy metals, using marine bivalve molluscs as raw materials. Background technique [0002] With the process of industrialization in our country, a large amount of industrial and agricultural wastewater has been discharged into natural water bodies, causing serious pollution in coastal waters, among which heavy metal ions have seriously polluted water bodies. When the environment is polluted by heavy metals, it can not only affect the physiological functions of marine organisms such as feeding, growth and reproduction, but also accumulate in the organisms and enter the human body through the food chain to pose a potential threat, causing acute and chronic poisoning, such as "Minamata disease" ", "Osteoarthritis", "leukopenia" an...

AI Technical Summary

Problems solved by technology

[0004] At present, most common MTs in the domestic market are purified from rabbit liver. Due to the special demand for MT raw materials, and the extraction technology of rabbit liver MT usually adopts buffer extraction, organic solvent precipitation to remove impurities, heat denaturation to remove impurities, and ethanol precipitation MT. and other processes, due to the use of a large amount of ethanol and chloroform in the process, not only the production process is cumbersome, but there is no effective method to produce MT in large quantities, which makes the price of MT extremely expensive and pollutes the environment, thereby limiting its application and research. MT raw materials Sources have become one of the bottlenecks restricting the development of this field