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Marine organism source superoxide dismutase extraction and separation process

A technology of superoxide and marine organisms, applied in the direction of oxidoreductase, biochemical equipment and methods, enzymes, etc., can solve the problems of increased risk of animal-derived blood products, cross-infection, allergic reactions, reagent waste removal process, etc. , to achieve the effects of low product purification cost, easy industrial production, and easy operation

Inactive Publication Date: 2019-04-16
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the extraction and purification process of traditional SOD, a large amount of organic solvent acetone and inorganic salt sulfuric acid are used respectively. The addition of these reagents not only affects the activity of the enzyme, but also increases the industrial cost of the waste of reagents and the subsequent removal process.
And animals themselves carry various bacterial viruses, etc., which inevitably lead to some cross-infection, allergic reactions and other phenomena, especially the spread of foot-and-mouth disease, mad cow disease and malignant infectious diseases transmitted by animals. People are interested in extracting from pigs and cattle. The safety of active ingredients such as SOD has been questioned, and the risk of producing animal-derived blood products has increased. Many countries and regions have boycotted superoxide dismutase produced from animal blood and viscera, making superoxide dismutase internationally popular. extremely scarce in the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A process for extracting and separating superoxide dismutase from marine biological sources, specifically comprising the following steps:

[0041] S1. Take fresh sea fish, take out the viscera and fish blood, clean the viscera, drain and set aside, add 5-10% sodium citrate of fish blood quality to the fish blood, mix well and refrigerate for use;

[0042] S2. Beating the cleaned viscera to obtain viscera slurry;

[0043] S3. Add phosphate buffer solution to the viscera slurry, adjust the pH of the extract to 6.5-7.5, stir and extract at 40-60°C for 2-6 hours, and obtain the viscera extract;

[0044] In the step S3, the amount of the phosphate buffer solution added is 6-10 L per kilogram of viscera slurry.

[0045] S4. Heating the viscera extract to remove impurities, and then centrifuging to remove the precipitate to obtain supernatant A;

[0046] In the step S4, the extract is heated to remove part of the impurity proteins, the heating temperature is 60-80° C., and t...

Embodiment 2

[0061] A process for extracting and separating superoxide dismutase from marine biological sources, specifically comprising the following steps:

[0062] S1. Take fresh sea fish, take out the viscera and fish blood, clean the viscera, drain and set aside, add 5-10% sodium citrate of fish blood quality to the fish blood, mix well and refrigerate for use;

[0063] S2. Beating the cleaned viscera to obtain viscera slurry;

[0064] S3. Add phosphate buffer solution to the viscera slurry, adjust the pH of the extract to 6.5-7.5, stir and extract at 40-60°C for 2-6 hours, and obtain the viscera extract;

[0065] In the step S3, the amount of the phosphate buffer solution added is 6-10 L per kilogram of viscera slurry.

[0066] S4. Heating the viscera extract to remove impurities, and then centrifuging to remove the precipitate to obtain supernatant A;

[0067] In the step S4, the extract is heated to remove part of the impurity proteins, the heating temperature is 60-80° C., and t...

Embodiment 3

[0082] A process for extracting and separating superoxide dismutase from marine biological sources, specifically comprising the following steps:

[0083] S1. Take fresh sea fish, take out the viscera and fish blood, clean the viscera, drain and set aside, add 5-10% sodium citrate of fish blood quality to the fish blood, mix well and refrigerate for use;

[0084] S2. Beating the cleaned viscera to obtain viscera slurry;

[0085] S3. Add phosphate buffer solution to the viscera slurry, adjust the pH of the extract to 6.5-7.5, stir and extract at 40-60°C for 2-6 hours, and obtain the viscera extract;

[0086] In the step S3, the amount of the phosphate buffer solution added is 6-10 L per kilogram of viscera slurry.

[0087] S4. Heating the viscera extract to remove impurities, and then centrifuging to remove the precipitate to obtain supernatant A;

[0088] In the step S4, the extract is heated to remove part of the impurity proteins, the heating temperature is 60-80° C., and t...

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PUM

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Abstract

The invention discloses a marine organism source superoxide dismutase extraction and separation process. The process comprises the following steps of: carrying out coarse extraction on viscera and fish blood respectively, purifying extracting solutions obtained by centrifugation, the superoxide dismutase prepared by extraction has high purity and high yield, and the unit enzyme activity of the superoxide dismutase can reach more than 6000U / mg. When the coarse extraction is carried out on the viscera and the fish blood, the use of a large amount of organic solvents and inorganic salt ammonium sulfate in a traditional extraction method is changed, the influence on the activity and safety of the superoxide dismutase caused by the addition of the reagents is reduced, but also the waste of reagentsand the subsequent removal process are reducedto reduce the industrial cost. The raw materials are viscera and fish blood of sea fish, so that environmental pollution and resource waste caused bya large amount of by-products generated by processing fish products are effectively reduced, waste utilization is achieved, and the waste is changed into valuable.

Description

technical field [0001] The invention relates to the technical field of extraction and preparation of superoxide dismutase, in particular to an extraction and separation process of superoxide dismutase from marine biological sources. Background technique [0002] Superoxide dismutase, alias liver protein, abbreviation: SOD. SOD is an active substance derived from living organisms, which can eliminate harmful substances produced by organisms during metabolism. Supplementing the human body continuously with SOD has a special anti-aging effect. SOD was first isolated from bovine red blood cells in 1938, and people have studied SOD for more than 70 years. In 1969, McCord et al. rediscovered this protein, discovered their biological activity, and clarified its nature of catalyzing the disproportionation reaction of peroxide anions, so it was officially named superoxide dismutase. [0003] Superoxide dismutase is a new type of enzyme preparation. It is widely distributed in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 洪鹏志周春霞刘唤明杨萍邓楚津
Owner GUANGDONG OCEAN UNIVERSITY
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