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Method for rapidly evaluating resistance of bivalve mollusks based on tension index

A technology of bivalve mollusk and pulling force, which is applied in application, climate change adaptation, fish farming, etc., can solve problems such as difficulty in accurately reflecting shellfish tolerance, cumbersome detection of heart rate indicators, and susceptibility to other factors. Achieve the effect of simple detection success rate

Active Publication Date: 2022-03-11
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the evaluation of shellfish resistance traits is generally evaluated by half-lethal rate or survival rate, but this index is easily affected by other factors, and it is difficult to accurately reflect the tolerance of shellfish
In the existing research, there is a method of using the heart rate index as a non-destructive live detection index for evaluating the resistance of scallops (Chinese invention patent with the publication number of CN 105165675A), but the detection operation of the heart rate index is relatively cumbersome and requires high measurement conditions

Method used

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  • Method for rapidly evaluating resistance of bivalve mollusks based on tension index
  • Method for rapidly evaluating resistance of bivalve mollusks based on tension index
  • Method for rapidly evaluating resistance of bivalve mollusks based on tension index

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Experimental program
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Effect test

Embodiment 1

[0041] In September 2020, 50 24-month-old Chlamys farreri were taken from the Laoshan sea area of ​​Qingdao for standardized testing of tensile indicators. The specific steps are as follows:

[0042] 1. Standardized determination of scallop pulling force:

[0043] 1) Place 50 24-month-old clam scallops in the laboratory inflated circulating seawater system for more than 10 minutes.

[0044]2) Adjust the upper and lower clips of the tension measuring device to a distance of 0.75cm, set the reading of the digital display tension meter to zero, adjust the unit to N (1N=0.98kg), and adjust the sampling rate to 1000Hz (5 data / second), Connect the serial port, and conduct an online test through the data analysis software to confirm that the device and the software are successfully connected.

[0045] 3) Take the scallop to be tested out of the seawater, keep its shell open, and quickly place it on the adjusted tension measuring device. After the scallop double shell is stably clamp...

Embodiment 2

[0070] Using the method of the present invention, another 50 18-month-old scallops cultured in the sea area of ​​Qingdao were tested to verify the accuracy and reliability of the method of the present invention. Specific steps are as follows:

[0071] 1. Standardized determination of scallop pulling force:

[0072] 1) Place 50 18-month-old clam scallops in the laboratory inflated circulating seawater system for more than 10 minutes.

[0073] 2) Adjust the upper and lower clips of the tension measuring device to a distance of 0.75cm, set the reading of the digital display tension meter to zero, adjust the unit to N (1N=0.98kg), and adjust the sampling rate to 1000Hz (5 data / second), Connect the serial port, and conduct an online test through the data analysis software to confirm that the device and the software are successfully connected.

[0074] 3) Take the scallop to be tested out of the seawater, keep its shell open, and quickly place it on the adjusted tension measuring ...

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Abstract

The invention provides a method for quickly evaluating the resistance of bivalve mollusks based on a tension index, which is a method for detecting the tension index without damage on the basis of not influencing the activity of a tested individual and quickly evaluating the resistance of scallops based on the tension index. According to the method, efficient and lossless tension detection is carried out on the scallops, and the obtained four tension indexes including the total force, the shell closing time, the average force and the maximum force of the scallops serve as the basis for rapidly evaluating the scallop resistance. Compared with the traditional method, the method provided by the invention can realize nondestructive living body detection, and compared with a concentricity detection method, the method provided by the invention has the characteristics of being more stable, accurate, simple, convenient, high in detection success rate and the like. In addition, the tension of the adductor muscle of the scallop is used as an index for rapidly evaluating the resistance of the scallop for the first time. The method provided by the invention can provide reference for scallop resistance index determination, and provides technical support for subsequent breeding of scallop stress-resistant varieties.

Description

technical field [0001] The invention belongs to the technical field of marine biological breeding, and in particular relates to a method for quickly evaluating the resistance of bivalve molluscs based on a pulling force index. Background technique [0002] The adductor muscle of bivalve molluscs is mainly composed of striated muscle, smooth muscle and ligament, which are closely related to the body's strength, escape behavior, and auxiliary feeding. Among them, the ligament is responsible for opening the double shell when the muscles are relaxed; the striated muscle quickly closes the double shell through rapid contraction; the contraction of the smooth muscle makes the double shell close slowly and can maintain a closed state for a long time. Adductor muscle tension refers to a quantitative index of the adductor muscle contraction ability of bivalve molluscs in the process of bivalve closure. Some studies have found that the change of bivalve pulling force index is signifi...

Claims

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Application Information

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IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 黄晓婷曾祥意杨祖晶隋明益邢强陆维包振民
Owner OCEAN UNIV OF CHINA
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