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Method for determining biotoxicity of water quality based on zebrafish movement velocity change

A technology of movement speed and biological toxicity, which is applied in the direction of water testing, general water supply saving, material inspection products, etc., can solve the problems of insensitivity, consumption of test materials, and inaccurate results, so as to improve timeliness and ensure the safety of water supply quality Effect

Inactive Publication Date: 2015-08-12
SHANDONG JIANZHU UNIV +1
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Problems solved by technology

At present, most detection methods use biological monitoring equipment for water quality biological toxicity, but only for comprehensive index evaluation, which is the result of the overall effect of pollutants on aquatic organisms, which consumes a lot of test materials, and it is easy to ignore its impact on organisms. Some aspects of the body itself, resulting in inaccurate and insensitive results, while wasting a lot of manpower and material resources

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  • Method for determining biotoxicity of water quality based on zebrafish movement velocity change
  • Method for determining biotoxicity of water quality based on zebrafish movement velocity change

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Embodiment 1

[0027] A method for measuring water quality biological toxicity based on the change of zebrafish movement speed, comprising the following steps:

[0028] (1) Put the zebrafish in standard dilution water with room temperature of 25°C and continuous aeration, feed and remove feces and food residues every day. The domestication time is 7 days, and the feeding is stopped the day before the test; the mortality of the zebrafish must be guaranteed during the domestication period Less than 10%, if the mortality rate of zebrafish is higher than 10%, then replace a batch of fish domestication;

[0029] (2) The domesticated zebrafish were placed in standard dilution water and different concentrations of Zn prepared in standard dilution water 2+ In solution, Zn 2+ The concentration of the solution was 10mg / L, 15mg / L, 20mg / L, 40mg / L and 60 mg / L, a total of 6 groups of experiments, each group set up 3 parallel groups, the number of zebrafish in each group was 5, Zn 2+ In the acute toxicit...

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Abstract

The invention discloses a method for determining biotoxicity of water quanlity based on movement velocity change of zebrafish. The method comprises the following steps: domesticating zebrafish; carrying out acute toxicity test on the domesticated zebrafish and calculating the 48 hour medial lethal concentration; and carrying out behavior test on the domesticated zebrafish, determining the movement velocity change of the zebrafish and judging the biotoxicity of water quality. With movement velocity of the zebrafish in a water environment as a biomarker, the biotoxicity of pollutants is evaluated; the movement behavior of the zebrafish in the water environment is tracked and captured; the movement track of the zebrafish is visually observed at any time; the speed index is determined in real time; quantitative evaluation for determining the biotoxicity of water quality by employing the zebrafish movement velocity change is realized; water quality fluctuation is displayed within a short period of time; the biological monitoring and early warning timeliness, sensitivity and accuracy are improved; a technical support is provided for sudden water body pollution; and the water supply quality safety is ensured.

Description

technical field [0001] The invention relates to the field of online water quality monitoring biological fish, in particular to a method for measuring water quality biological toxicity based on the change of zebrafish movement speed, and belongs to the field of environmental monitoring. Background technique [0002] Water quality monitoring technology involves water quality monitoring and early warning in the process of urban water supply and water quality management, which can predict sudden pollution events in advance and provide emergency treatment measures. The impact level of pollutants on aquatic organisms ranges from biomolecules to organelles, cells, tissues, organs, organ systems, individual organisms, biological populations, biological communities, and even the entire ecosystem. Water quality monitoring based on physiological and biochemical changes can realize biological toxicity assessment of water quality in water environment. [0003] The on-line monitoring of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
CPCY02A20/20
Inventor 李梅贾瑞宝李汝宋武昌逯南南张克峰
Owner SHANDONG JIANZHU UNIV
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