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Resonance scattering spectrometry for detecting trace of albumin in human urine

A microalbumin and resonant scattering technology, applied in the field of human urine microalbumin determination, can solve the problems of complex operation, long time-consuming, harmful to human body, etc., and achieve the effect of good catalytic performance, simple operation and simple instrument

Inactive Publication Date: 2008-12-10
GUANGXI NORMAL UNIV
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Problems solved by technology

Among these methods, although some are easy to operate, the sensitivity is not high, and some are high-sensitivity, but the operation is complicated and time-consuming, and some use toxic substances, which not only pollute the environment, but also are harmful to the human body.
Therefore, it is of great significance to study new methods for the detection of human urinary microalbumin, but there is no report on the detection of human urinary microalbumin by immunogold catalysis-cuprous oxide enhanced resonance scattering spectroscopy

Method used

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  • Resonance scattering spectrometry for detecting trace of albumin in human urine
  • Resonance scattering spectrometry for detecting trace of albumin in human urine
  • Resonance scattering spectrometry for detecting trace of albumin in human urine

Examples

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Embodiment Construction

[0022] Use the method of the present invention to detect the content of urinary microalbumin in 10 healthy people's urine.

[0023] Cary ecupse fluorescence instrument (Varian, USA); TU-1901 double-beam UV-visible spectrophotometer (Beijing General Instrument Co., Ltd.); TGL-high-speed refrigerated centrifuge (Shanghai Anting Instrument Co., Ltd.); SK8200LH ultrasonic Reactor (Shanghai Kedao Ultrasonic Instrument Co., Ltd.); H-600 Transmission Electron Microscope (JEOL Ltd.).

[0024] 240mg / L urinary microalbumin (Malb) standard substance, goat anti-human urinary microalbumin antibody (Shanghai Jiemen Biotechnology Co., Ltd.), HAuCl 4 (Sinopharm Chemical Reagent Company), sample urine (The Fifth People's Hospital of Guilin City), 0.20mol / LNa 2 HPO 4 , 0.10mol / L citric acid solution, 0.10mol / L K 2 CO 3 , 100mg / mL KCl, 69.2mg / mL CuSO 4 , 250mg / mL NaOH (containing 346mg / mL potassium sodium tartrate), 1mg / mL glucose solution, all reagents used are of analytical grade, and the...

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Abstract

The utility model discloses a resonance scattering spectroscopy method for quickly and sensitively detecting microalbumin in human urine. According to the method provided by the utility model, the detection system of Malb the concentration of which is known is firstly prepared to work out the resonance scattering spectroscopy I 610nm of Malb; and then Mabl can be taken as a blank and the blank value (I 610) b can be measured, and Delta I can be worked out according to the equation: Delta I is equal to I 610nm subtracting (I 610) b; a working curve can be drawn by taking the concentration of added Malb solution as a horizontal axis, and taking Delta I as a vertical axis; and then, the system to be detected of a detecting sample can be prepared, and the Delta I of a sample to be detected can be worked out; and the Malb concentration of the sample can be also worked out according to the working curve. The method provided by the utility model has the advantages that instruments included in the method are simple and can be operated easily; film regent is stable and suitable for storage; colloidal gold can be prepared easily; catalysis performance is excellent and quick; and the sensitivity is high.

Description

Technical field: [0001] The invention relates to a method for measuring human urinary microalbumin, in particular to a resonance scattering spectrometry method for detecting human urinary microalbumin. Background technique: [0002] Nanogold has the characteristics of easy preparation, high electron density, unique optical and electrical properties, and good biocompatibility. It has been used in protein labeling, nucleic acid labeling, biosensors, and biochips. Recently, it has been found that gold nanoparticles have strong catalytic activity and have broad application prospects in biochemical analysis. Resonance scattering spectroscopy has the characteristics of sensitivity, simplicity, and speed, and has been applied to the analysis of proteins, nucleic acids, and inorganic ions. [0003] Urinary microalbumin (Malb) is an indicator of vascular damage in diabetes, hypertension, and cardiovascular disease. Its determination has epoch-making significance for early diagnosis ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/25G01N21/65G01N21/51
Inventor 黄玉娟刘庆业蒋治良
Owner GUANGXI NORMAL UNIV
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