Chemiluminescence immune detection kit for detecting pro-gastrin-releasing peptide

A chemiluminescent immunoassay and gastrin-releasing peptide technology, applied in the field of immunoassay, can solve the problems of poor sensitivity and accuracy, slow detection speed, etc., and achieve the effect of simple operation, simple instrument and low detection cost

Inactive Publication Date: 2015-11-18
NANJING WENZHI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection speed of ELISA and RIA detection methods is relatively slow, and the sensitivity and accuracy of detection are also poor

Method used

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  • Chemiluminescence immune detection kit for detecting pro-gastrin-releasing peptide
  • Chemiluminescence immune detection kit for detecting pro-gastrin-releasing peptide

Examples

Experimental program
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Embodiment 1

[0040] 1. Preparation of each component of the kit

[0041] (1) Preparation of proGRP hapten: acidify proGRP, react with sodium nitrite in a low-temperature environment without light at 4°C (the dosage ratio of the two is 1:1), and generate an intermediate containing diazonium cations. The diazotized proGRP is used as a hapten for subsequent synthesis of immune antigens and coating antigens;

[0042] (2) Preparation of proGRP-bovine serum albumin (BSA) immunogen: proGRP and bovine serum albumin (BSA) were coupled by a diazotization method to obtain proGRP-BSA as an immune antigen;

[0043] (3) Preparation of proGRP-oval serum albumin (OVA) coated antigen: proGRP and egg serum albumin (OVA) were coupled by diazotization method to obtain proGRP-oval serum albumin (OVA) conjugate, as a coating antigen;

[0044](4) Preparation of proGRP-peroxidase-labeled antibody: For 6-8 week-old female BALB / c mice (body weight 18-20g), the high-dose immunization scheme is as follows: 160 μg p...

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Abstract

The invention provides a chemiluminescence immune detection kit for detecting a pro-gastrin-releasing peptide; the chemiluminescence immune detection kit is composed of a non-transparent white elisa plate coated with the pro-gastrin-releasing peptide, a pro-gastrin-releasing peptide standard, a pro-gastrin-releasing peptide-peroxidase labelled antibody working liquid, a luminous substrate liquid, a concentrated sample diluent and a concentrated detergent. A pro-gastrin-releasing peptide protein conjugate is obtained by coupling the pro-gastrin-releasing peptide with a carrier protein by a mixed anhydride method or a carbodiimide method, and the concentrated detergent contains 0.05% of Twain-20. Compared with a conventional enzyme-linked immunosorbent assay, the kit has higher sensitivity, is short in detection time and low in cost, and can be used for detection of the pro-gastrin-releasing peptide in human blood or serum.

Description

technical field [0001] The invention relates to the field of immune analysis, in particular to a chemiluminescence immunoassay kit for detecting gastrin releasing peptide precursor. Background technique [0002] Lung cancer is a highly malignant neoplastic disease, and the growth rate of its incidence is also the highest among all malignant tumors. Among them, small cell lung cancer (small cell lung cancer, SCLC) accounts for 15% to 20% of lung cancers. It is a special lung tumor with high malignancy, rapid growth and easy early metastasis. SCLC has the characteristics of high growth fraction and short doubling time. Most patients are in the advanced stage when they are diagnosed. The five-year survival rate is low and the prognosis is poor. Therefore, early diagnosis and early treatment of SCLC are particularly important. With the development of chemistry and molecular biology, tumor markers (tumormarker, TM) have become one of the important means of diagnosing malignant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 杨子学滕娇
Owner NANJING WENZHI BIOTECH
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