Preparation of chemiluminescence immunoassay kit for detecting chloroquine

Abstract

The invention provides a preparation of a chemiluminescence immunoassay kit for detecting chloroquine and belongs to the field of immunological detection. The kit is composed of: a non-transparent white enzyme label plate coated by a chloroquine-carrier protein conjugate, a chloroquine standard substance, a chloroquine-peroxidase marked antibody operating fluid, a luminescent substrate liquid, a concentrated sample diluting liquid and a concentrated washing liquid. The chloroquine-carrier protein conjugate is prepared by coupling chloroquine to a carrier protein in a mixed anhydride method or a carbodiimide method. The concentrated washing liquid contains 0.05% of tween-20. The kit is higher in sensitivity, is short in detection time, is low in cost and can be used for detection of residual amount of the chloroquine in samples such as fruits, crops and vegetables and the like.

Description

technical field

[0001] The invention relates to a chemiluminescent immunoassay kit for detecting chloroquine, which is used for detecting the content or residual amount of chloroquine in fruits, vegetables, crops and the like. It belongs to the field of immunological detection. Background technique

[0002] Chloroquine metabolizes slowly in the human body, and when the content exceeds a certain amount, it is easy to cause reactions such as loss of appetite, nausea and vomiting, and diarrhea; skin itching, purpura, hair loss, whitening of hair, eczema, exfoliative dermatitis, and psoriasis can also occur; heavy head, Headache, dizziness, tinnitus, vertigo, fatigue, sleep disturbance, mental confusion, reduced vision, corneal and retinal degeneration, etc.

[0003] Chloroquine residue analysis generally uses gas chromatography (GC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC / MS). These methods are sensitive and accurate, and can...